| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,294,423 | C→T | 49.6% | A274A (GCG→GCA) | nac ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,294,423 | 0 | C | T | 49.6% | ‑0.5 / 131.1 | 139 | A274A (GCG→GCA) | nac | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (36/34); new base T (37/32); total (73/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
Only 100 of 139 total aligned reads displayed.
ACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCA > minE/1294361‑1294488 | aCTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTg > 1:471948/1‑69 (MQ=255)aCTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTg > 1:1374557/1‑69 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCg > 1:1345410/1‑61 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTaaa > 1:1887239/1‑67 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTaaa > 1:3240263/1‑67 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTaaa > 1:1985688/1‑67 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTaaa > 1:143635/1‑67 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:2200556/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:2576803/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:3383063/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:2362576/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:1141716/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:765992/1‑68 (MQ=255) aaTCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAt > 1:1056387/1‑68 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:1831097/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:2006242/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:2002352/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:2557345/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:1614331/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:2618313/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:350005/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:372293/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:403133/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGTGACAAGTTTGCTCTGGCGGGTAAAttt < 1:470355/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAAttt < 1:3297362/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAAttt < 1:450242/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAAttt < 1:1021220/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAAttt < 1:94592/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAAttt < 1:1662947/67‑1 (MQ=255) cACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGAGGGTAAAttt < 1:2506822/67‑1 (MQ=255) aCTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAAt > 1:2229725/1‑69 (MQ=255) aCTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAAt > 1:2286988/1‑69 (MQ=255) aCTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAAt > 1:3407443/1‑69 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa < 1:844453/69‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa < 1:3376550/69‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa < 1:371605/69‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa < 1:748245/69‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCCGGTAAATTTAATGACaaa < 1:2236309/69‑1 (MQ=255) tAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:1108862/69‑1 (MQ=255) aacaacTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa > 1:3302679/1‑66 (MQ=255) aacaacTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa > 1:2384591/1‑66 (MQ=255) aacaacTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa > 1:755710/1‑66 (MQ=255) aacaacTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa > 1:1751982/1‑66 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:32009/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:1377795/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:2832004/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:2587328/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:649082/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:2110337/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:860750/1‑68 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:490750/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1480796/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1655149/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:782788/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1141889/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:2934565/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:837029/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1726422/69‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:586339/46‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:583241/46‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:3408701/46‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:873590/46‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGt < 1:3282400/67‑1 (MQ=255) gCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGt > 1:3127008/1‑61 (MQ=255) gCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGt < 1:1328243/68‑1 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:2910214/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:2483998/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:2422919/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:2769092/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:1079292/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:386658/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:210027/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:1675773/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:2949382/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:2971157/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:3071239/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:940553/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAAt > 1:883800/3‑61 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGAc > 1:110306/3‑68 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1780949/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:2291214/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1707178/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:2447406/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:2558939/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1520693/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:47985/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:3027479/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1251145/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGAGGTAATGCGTGACa > 1:3345797/3‑69 (MQ=255) acaaGTTTGATCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1473342/3‑69 (MQ=255) caaGTTTGCTCTGGCGTGTAAATTTAAGGACAAAGAGAGACTc < 1:1066452/42‑1 (MQ=255) aaGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATc > 1:1248245/1‑69 (MQ=255) aaGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATc > 1:1618141/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTaa > 1:1253904/1‑56 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACa > 1:823143/1‑65 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:1896914/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:2886081/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:92052/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:921735/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:572479/1‑69 (MQ=255) | ACTGGAGAACTAATCACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCA > minE/1294361‑1294488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |