| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,294,444 | T→G | 47.7% | S267S (TCA→TCC) | nac ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,294,444 | 0 | T | G | 47.7% | 0.3 / 152.2 | 130 | S267S (TCA→TCC) | nac | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (41/27); new base G (21/41); total (62/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.97e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 133 total aligned reads displayed.
ACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATA > minE/1294376‑1294508 | aCTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAAt > 1:2286988/1‑69 (MQ=255)aCTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAAt > 1:2229725/1‑69 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa < 1:3376550/69‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa < 1:371605/69‑1 (MQ=255) aTTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa < 1:844453/69‑1 (MQ=255) aacaacTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa > 1:3302679/1‑66 (MQ=255) aacaacTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACaaa > 1:755710/1‑66 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:2587328/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:2110337/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:3182513/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:649082/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:2976663/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:1377795/1‑68 (MQ=255) ttCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCAt > 1:2832004/1‑68 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:782788/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:837029/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1480796/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:873604/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1141889/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1655149/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:2934565/69‑1 (MQ=255) tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1726422/69‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:873590/46‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:583241/46‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAaga < 1:3408701/46‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGt < 1:3282400/67‑1 (MQ=255) gCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGt > 1:3127008/1‑61 (MQ=255) gCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGt > 1:2555794/1‑61 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAGGAGAGACTc < 1:2074598/42‑1 (MQ=25) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:1693176/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:3425525/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:386658/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:2483998/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:2422919/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:2971157/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:940553/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:2949382/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:2910214/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTc < 1:1079292/42‑1 (MQ=37) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAAt > 1:883800/3‑61 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGAc > 1:110306/3‑68 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1068813/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:2558939/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:92250/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:764479/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:3027479/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:47985/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:377712/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1251145/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1520693/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1707178/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:2380009/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1780949/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1866301/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:2282731/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:2291214/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGAGGTAATGCGTGACa > 1:3345797/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTAATTGAAGGAGTGGTAATGCGTGACa > 1:47611/3‑69 (MQ=255) acaaGTTTGATCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1473342/3‑69 (MQ=255) caaGTTTGCTCTGGCGTGTAAATTTAAGGACAAAGAGAGACTc < 1:1066452/42‑1 (MQ=255) aaGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATc > 1:1618141/1‑69 (MQ=255) aaGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATc > 1:2136766/1‑69 (MQ=255) aaGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATc > 1:1248245/1‑69 (MQ=255) aaGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATc > 1:3286672/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTaa > 1:1253904/1‑56 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACa > 1:823143/1‑65 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:2942254/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:572479/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:2886081/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:1896914/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:921735/1‑69 (MQ=255) ttCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:1636824/1‑68 (MQ=255) tCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:2741625/46‑1 (MQ=255) gTAAATTTAAGGACAAAGAGAGACTCAT‑TAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:296263/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:1200065/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:1421030/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:1433109/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:11547/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:675859/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:1516643/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:590118/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2392564/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:3389385/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2194923/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2170328/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:3108918/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2146718/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2346250/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2822231/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:376162/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2772046/69‑1 (MQ=255) aTTTAAGGACAAAGAGAGAGTCATTGAAGGAGTTGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2080230/66‑1 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTAc > 1:2387542/1‑58 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACAt > 1:260034/1‑68 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACAt > 1:471581/1‑68 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:1497334/1‑69 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:1820371/1‑69 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:536392/1‑69 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:2327394/1‑69 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:1528204/1‑69 (MQ=255) | ACTGACATTAACAACTCTTTCACTGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATA > minE/1294376‑1294508 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |