| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,294,462 | G→T | 49.1% | S261S (TCC→TCA) | nac ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,294,462 | 0 | G | T | 49.1% | 15.7 / 100.0 | 92 | S261S (TCC→TCA) | nac | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base G (32/14); new base T (21/24); total (53/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.43e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCG > minE/1294399‑1294519 | tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1726422/69‑1 (MQ=255)tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1141889/69‑1 (MQ=255)tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1655149/69‑1 (MQ=255)tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:2845626/69‑1 (MQ=255)tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:873604/69‑1 (MQ=255)tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:837029/69‑1 (MQ=255)tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:782788/69‑1 (MQ=255)tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:2934565/69‑1 (MQ=255)tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:1480796/69‑1 (MQ=255)tgcctgtgcctgTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:490750/69‑1 (MQ=255) gcctgtgcctgtGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:965572/68‑1 (MQ=255) cctgtgGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGt < 1:3282400/67‑1 (MQ=255) gCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGt > 1:3127008/1‑61 (MQ=255) gCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGt > 1:2555794/1‑61 (MQ=255) gCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGt < 1:1328243/68‑1 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAAt > 1:883800/3‑61 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGAc > 1:110306/3‑68 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:47985/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:377712/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:2447406/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:3027479/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:764479/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:2558939/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:2380009/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:92250/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:2291214/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:2282731/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1866301/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1068813/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1520693/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1251145/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1707178/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1780949/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGAGGTAATGCGTGACa > 1:3345797/3‑69 (MQ=255) acaaGTTTGCTCTGGCGGGTAAATTTAAGGACAAAGAGAGACTAATTGAAGGAGTGGTAATGCGTGACa > 1:47611/3‑69 (MQ=255) acaaGTTTGATCTGGCGGGTAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACa > 1:1473342/3‑69 (MQ=255) aaGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATc > 1:1248245/1‑69 (MQ=255) aaGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATc > 1:120414/1‑69 (MQ=255) aaGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATc > 1:2136766/1‑69 (MQ=255) aaGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATc > 1:3286672/1‑69 (MQ=255) aaGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATc > 1:1618141/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTaa > 1:1253904/1‑56 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACa > 1:823143/1‑65 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:2886081/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:1896914/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:1448510/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:921735/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:2942254/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:572479/1‑69 (MQ=255) gTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:92052/1‑69 (MQ=255) ttCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCa > 1:1636824/1‑68 (MQ=255) tCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAgg < 1:2741625/46‑1 (MQ=255) gTAAATTTAAGGACAAAGAGAGACTCAT‑TAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:296263/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:11547/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:3389385/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:296596/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:371869/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:376162/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:1433109/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:1421030/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:590118/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:675859/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:1200065/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2146718/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2346250/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2392564/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2298872/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2194923/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2170328/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2156678/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2772046/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2822231/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:293660/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:1516643/69‑1 (MQ=255) tAAATTTAAGGACAAAGAGAGACTCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:3108918/69‑1 (MQ=255) aTTTAAGGACAAAGAGAGAGTCATTGAAGGAGTTGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:2080230/66‑1 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTAc > 1:2387542/1‑58 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACAt > 1:471581/1‑68 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACAt > 1:260034/1‑68 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:1820371/1‑69 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:1877396/1‑69 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:1497334/1‑69 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:2327394/1‑69 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:536392/1‑69 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:1576780/1‑69 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:465831/1‑69 (MQ=255) ttAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATa > 1:1528204/1‑69 (MQ=255) gagagaCTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAAcgcgcg > 1:882131/1‑69 (MQ=255) gagagaCTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAAcgcgcg > 1:2628413/1‑69 (MQ=255) gagagaCTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAAcgcgcg > 1:396063/1‑69 (MQ=255) gagaCTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATaacg > 1:1848551/1‑59 (MQ=255) tCATTGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGcc < 1:1052129/46‑1 (MQ=255) | TGCCTGTGCCTGTGGCGATAAGTTCGCTCTGGCGGGTAAATTTAATGACAAAGAGAGACTCATGGAAGGAGTGGTAATGCGTGACATCCACCCATTTACTGCGCCACATAACGAACGCGCG > minE/1294399‑1294519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |