| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,294,615 | T→C | 23.7% | R210R (CGA→CGG) | nac ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,294,615 | 0 | T | C | 23.7% | 98.0 / 25.8 | 82 | R210R (CGA→CGG) | nac | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (16/45); new base C (7/12); total (23/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01 | |||||||||||
CCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGA > minE/1294550‑1294679 | ccGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGc > 1:1369065/1‑69 (MQ=255)ccGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGc > 1:187022/1‑69 (MQ=255) cGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGaaaa > 1:2971001/1‑69 (MQ=255) gCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGaaaa > 1:1400116/1‑68 (MQ=255) aGCGTGGCAATAGACT‑AATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCaa < 1:1301551/69‑1 (MQ=255) cGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCaa < 1:205061/68‑1 (MQ=255) cGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCaa < 1:3140762/68‑1 (MQ=255) cGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCaa < 1:3338243/68‑1 (MQ=255) cGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCaa < 1:2620791/68‑1 (MQ=255) cGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCaa < 1:1061650/68‑1 (MQ=255) cGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCaa < 1:2357422/68‑1 (MQ=255) cGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCaa < 1:2013503/68‑1 (MQ=255) cGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCaa < 1:1670021/68‑1 (MQ=255) cGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCaa < 1:572622/68‑1 (MQ=255) cGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCaa < 1:1558851/68‑1 (MQ=255) aGACTCAATTTCCCCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt > 1:2912131/1‑69 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:1388017/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:2443931/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:252155/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:2526403/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt > 1:1368765/1‑69 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:769209/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:3431156/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:1384988/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:372162/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:1907580/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:3058253/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt > 1:527784/1‑69 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:1802428/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:1404593/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:676449/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:1656418/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:859412/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:613447/69‑1 (MQ=255) aGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:1429416/69‑1 (MQ=255) gACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAgg > 1:608015/1‑42 (MQ=255) gACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACg > 1:1384493/1‑62 (MQ=255) gACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACg > 1:2847459/1‑62 (MQ=255) gACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACg > 1:3422953/1‑62 (MQ=255) tCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:3065352/65‑1 (MQ=255) tCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:1851662/65‑1 (MQ=255) tCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:119881/65‑1 (MQ=255) tCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:2238902/65‑1 (MQ=255) tCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:2135045/65‑1 (MQ=255) tCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:2803054/65‑1 (MQ=255) cAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAg < 1:3423058/69‑1 (MQ=255) cAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAg < 1:732322/69‑1 (MQ=255) cAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAg < 1:3430730/69‑1 (MQ=255) cAATTTCACCAATAACTTTTGCCGTGAGACGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAg < 1:3300280/69‑1 (MQ=255) aaTTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGc > 1:2606110/1‑69 (MQ=255) aaTTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGc > 1:2929786/1‑69 (MQ=255) tttCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCAc < 1:2904942/69‑1 (MQ=255) tttCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCAc < 1:2462302/69‑1 (MQ=255) tttCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCAc < 1:242047/69‑1 (MQ=255) tttCACCAATAACTTTTGCCGTGAGGCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCt < 1:2848716/61‑1 (MQ=255) aCCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGt < 1:2770705/68‑1 (MQ=255) aCCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGt < 1:1560295/68‑1 (MQ=255) aaTAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATc < 1:2582921/69‑1 (MQ=255) aaTAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATc < 1:3008200/69‑1 (MQ=255) aTAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATc < 1:3279490/68‑1 (MQ=255) aCTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTa > 1:1292873/1‑62 (MQ=255) aCTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTa > 1:2392970/1‑62 (MQ=255) aCTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTa > 1:2288677/1‑62 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:508264/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:1148902/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:80109/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:1239962/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:622801/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:1837619/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:429097/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:3314151/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:315232/67‑1 (MQ=255) ttttGCCGTGAGCAGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTCACAGCGCTGTAATCACTg < 1:193745/67‑1 (MQ=255) ttttGCCGAGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:1413046/67‑1 (MQ=255) ctttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:2061566/66‑1 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:3129163/1‑67 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:509537/1‑67 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:1667411/1‑67 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:1540754/1‑67 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:1410566/1‑67 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:2658558/1‑67 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:2829861/1‑67 (MQ=255) | CCGCTGGCAATCGCTGCGGTAAGCGTGGCAATAGACTCAATTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGA > minE/1294550‑1294679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |