| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,294,657 | A→G | 33.5% | S196S (AGT→AGC) | nac ← | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,294,657 | 0 | A | G | 33.5% | 55.8 / 39.7 | 77 | S196S (AGT→AGC) | nac | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (34/16); new base G (13/12); total (48/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01 | |||||||||||
TTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTT > minE/1294590‑1294715 | tttCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCAc < 1:242047/69‑1 (MQ=255)tttCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCAc < 1:2462302/69‑1 (MQ=255)tttCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCAc < 1:2904942/69‑1 (MQ=255) aCCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGt < 1:2770705/68‑1 (MQ=255) aCCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGt < 1:1560295/68‑1 (MQ=255) aaTAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATc < 1:2582921/69‑1 (MQ=255) aaTAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATc < 1:3008200/69‑1 (MQ=255) aTAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATc < 1:3279490/68‑1 (MQ=255) aCTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTa > 1:2392970/1‑62 (MQ=255) aCTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTa > 1:2288677/1‑62 (MQ=255) aCTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTa > 1:1292873/1‑62 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:80109/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:1837619/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:315232/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:622801/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:1148902/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:3314151/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:1239962/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:429097/67‑1 (MQ=255) ttttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:508264/67‑1 (MQ=255) ttttGCCGTGAGCAGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTCACAGCGCTGTAATCACTg < 1:193745/67‑1 (MQ=255) ttttGCCGAGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:1413046/67‑1 (MQ=255) ctttGCCGTGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTg < 1:2061566/66‑1 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:1540754/1‑67 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:509537/1‑67 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:2658558/1‑67 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:2829861/1‑67 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:3129163/1‑67 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:1410566/1‑67 (MQ=255) tGAGCCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAACAGCGCTGTAATCACTGGGGAGaaaaa > 1:1667411/1‑67 (MQ=255) gAAAAAGCCTCATCAACACGAAGTCTAATAGCTCTGTAATCACTGGGGAGAAAGAGGTTCATTTgcgc < 1:200157/68‑1 (MQ=255) gAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTgcgc < 1:581660/68‑1 (MQ=255) caACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTcaca > 1:326691/1‑68 (MQ=255) caACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTcaca > 1:979945/1‑68 (MQ=255) caACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTcaca > 1:224224/1‑68 (MQ=255) caACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTcaca > 1:135880/1‑68 (MQ=255) caACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTcaca > 1:1094098/1‑68 (MQ=255) caACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTcaca > 1:1141663/1‑68 (MQ=255) caACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGATCATTTGCGCAATAGCATTcac > 1:2908018/1‑67 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGgaga > 1:3147351/1‑37 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGgaga > 1:1741398/1‑37 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGgaga > 1:2288255/1‑37 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGgaga > 1:1399485/1‑37 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGgaga > 1:2781195/1‑37 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGgaga > 1:1179890/1‑37 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAgg > 1:2883717/1‑43 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCCCATc > 1:1026622/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:1175499/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc < 1:1815171/69‑1 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc < 1:514258/69‑1 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:3385205/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:1088258/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:3308906/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:932475/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc < 1:3226331/69‑1 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:960593/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:2986692/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc < 1:2321251/69‑1 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc < 1:2440204/69‑1 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:2808529/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:1990888/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:2877775/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc < 1:1938604/69‑1 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:2322847/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:1932477/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:3055528/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:3074455/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc < 1:3074761/69‑1 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:3085988/1‑69 (MQ=255) aaCACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATc > 1:311959/1‑69 (MQ=255) gTCTAACAGCTCTGTAATCACTGGGGAGAAAAAGGTTCATTTGCGCAATAGCATTAACATCAACGCttt > 1:1194068/1‑69 (MQ=255) gTCTAACAGCGCTGTAATCACTGGGGAGAAAAAGGTTCATTTGCGCAATAGCATTAACATCAACGCttt > 1:561007/1‑69 (MQ=255) gTCTAACAGCGCTGTAATCACTGGGGAGAAAAAGGTTCATTTGCGCAATAGCATTAACATCAACGCttt > 1:1431482/1‑69 (MQ=255) gTCTAACAGCGCTGTAATCACTGGGGAGAAAAAGGTTCATTTGCGCAATAGCATTAACATCAACGCttt > 1:612716/1‑69 (MQ=255) gTCTAACAGCGCTGTAATCACTGGGGAGAAAAAGGTTCATTTGCGCAATAGCATTAACATCAACGCttt > 1:3227566/1‑69 (MQ=255) gTCTAACAGCGCTGTAATCACTGGGGAGAAAAAGGTTCATTTGCGCAATAGCATTAACATCAACGCttt > 1:2058155/1‑69 (MQ=255) gTCTAACAGCGCTGTAATCACTGGGGAGAAAAAGGTTCATTTGCGCAATAGCATTAACATCAACGCttt > 1:2281485/1‑69 (MQ=255) | TTTCACCAATAACTTTTGCCGTGAGTCGCCGTAGGGAAAAAGCCTCATCAACACGAAGTCTAATAGCACTGTAATCACTGGGGAGAAAGAGGTTCATTTGCGCAATAGCATTCACATCAACGCTTT > minE/1294590‑1294715 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |