| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,297,593 | C→T | 50.0% | A62A (GCG→GCA) | cobT ← | nicotinate‑nucleotide dimethylbenzimidazole‑P phophoribosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,297,593 | 0 | C | T | 50.0% | ‑4.8 / 59.5 | 66 | A62A (GCG→GCA) | cobT | nicotinate‑nucleotide dimethylbenzimidazole‑P phophoribosyl transferase |
| Reads supporting (aligned to +/- strand): ref base C (23/10); new base T (23/10); total (46/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01 | |||||||||||
ACTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGT > minE/1297528‑1297647 | aCTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGc > 1:2843745/1‑68 (MQ=255)aCTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCt > 1:2955996/1‑69 (MQ=255)aCTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCt > 1:1993747/1‑69 (MQ=255)aCTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCt > 1:2515818/1‑69 (MQ=255)aCTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCt > 1:2596375/1‑69 (MQ=255)aCTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCt > 1:815835/1‑69 (MQ=255) ttcttTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCttt < 1:229431/69‑1 (MQ=255) ttcttTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCttt < 1:445581/69‑1 (MQ=255) aGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCgg > 1:2746550/1‑69 (MQ=255) aGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCgg > 1:337751/1‑69 (MQ=255) aGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCgg > 1:123809/1‑69 (MQ=255) aGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCgg > 1:1922150/1‑69 (MQ=255) aGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCgg > 1:2486604/1‑69 (MQ=255) aGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCg > 1:835296/1‑68 (MQ=255) aGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACAAAACCAGTACCGCTTTTTTGCCCACATGCg > 1:216003/1‑68 (MQ=255) cccTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCgg < 1:2527165/63‑1 (MQ=255) cccTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCgg < 1:911597/63‑1 (MQ=255) cccTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCgg < 1:2058282/63‑1 (MQ=255) cccTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCgg < 1:692473/63‑1 (MQ=255) cccTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCgg < 1:1699751/63‑1 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:1205275/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:44520/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:3133027/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:3253395/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:335379/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:847932/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:3377417/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:772762/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:3413984/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:752279/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:749145/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:568629/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:379709/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:498439/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:402387/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:1450062/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:1990422/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:2086851/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:2340991/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:2769156/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAccc > 1:2711702/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCAATTCAAccc > 1:405480/1‑69 (MQ=255) ccAGACGCCGTGATCGGCACACATAACCAGTACTGCTTTTTTGCCCACATGAGGTATGCCATTCAAc > 1:3344968/1‑67 (MQ=255) aGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAAcccc > 1:1165675/1‑68 (MQ=255) aGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAAcccc > 1:1652305/1‑68 (MQ=255) aGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAAcccc > 1:2637117/1‑68 (MQ=255) aGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAAcccc > 1:3136575/1‑68 (MQ=255) cGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCAtt < 1:3029737/68‑1 (MQ=255) aTCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGc > 1:2953610/1‑47 (MQ=255) aTCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGc > 1:3357721/1‑47 (MQ=255) aTCAGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGc > 1:2037064/1‑47 (MQ=255) cGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCa > 1:1798852/1‑69 (MQ=255) cGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCa > 1:3249698/1‑69 (MQ=255) cGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCa > 1:2946529/1‑69 (MQ=255) cacaTAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGt < 1:710276/69‑1 (MQ=255) aTAACCAGTACTGCTTTTTTGCCCACATGCTGTATGCCATTCAACCCCgg < 1:467270/50‑1 (MQ=255) aTAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAAcccctg < 1:1798707/50‑3 (MQ=255) aTAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCgg < 1:689244/50‑1 (MQ=255) aTAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCgg < 1:2115229/50‑1 (MQ=255) aTAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCgg < 1:698927/50‑1 (MQ=255) aTAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCgg < 1:1746553/50‑1 (MQ=255) aTAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCgg < 1:1681633/50‑1 (MQ=255) aTAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCgg < 1:3311493/50‑1 (MQ=255) aTAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCgg < 1:1277719/50‑1 (MQ=255) aTAACCAGTACTGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCgg < 1:2942966/50‑1 (MQ=255) aCCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCa < 1:730128/40‑1 (MQ=255) ccGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCAt > 1:2202410/1‑43 (MQ=255) | ACTTCTTTTGGAGAAATAGCGACCCCTTCCTCCCAGACGCCGTGATCGGCACACATAACCAGTACCGCTTTTTTGCCCACATGCGGTATGCCATTCAACCCCGGCATTCCTGCCAGTTGT > minE/1297528‑1297647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |