| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 105,125 | C→T | 26.9% | Q389Q (CAG→CAA) | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 105,125 | 0 | C | T | 26.9% | 129.7 / 43.5 | 111 | Q389Q (CAG→CAA) | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base C (48/33); new base T (16/14); total (64/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
CCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCA > minE/105071‑105188 | ccAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGAc > 1:846685/1‑68 (MQ=255)ccAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGAc > 1:1699874/1‑68 (MQ=255)ccAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGAc > 1:2470663/1‑68 (MQ=255)ccAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGAc > 1:2242819/1‑68 (MQ=255)ccAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGAc > 1:3125170/1‑68 (MQ=255)ccAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGAc > 1:1322004/1‑68 (MQ=255)ccAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGAc > 1:1549764/1‑68 (MQ=255)ccAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGAc > 1:979819/1‑68 (MQ=255) cAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACgg < 1:1526424/69‑1 (MQ=255) gTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGa < 1:2989831/68‑1 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:3225873/1‑69 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:3252227/1‑69 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:1800863/1‑69 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:3401222/1‑69 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:379496/1‑69 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:2164822/1‑69 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:2203328/1‑69 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:1118783/1‑69 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:1623375/1‑69 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:356215/1‑69 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:1331568/1‑69 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:1511418/1‑69 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAtt > 1:1927227/1‑68 (MQ=255) aCCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACAACGCCTTGTTCCTGCTTGGCGCGACGGAAttt > 1:1804960/1‑69 (MQ=255) cccAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAAttt < 1:1997508/68‑1 (MQ=255) cAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCa < 1:2119328/68‑1 (MQ=255) cAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCa < 1:3321016/68‑1 (MQ=255) cAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCa < 1:800354/68‑1 (MQ=255) cAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCa < 1:902661/68‑1 (MQ=255) cAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCa < 1:922776/68‑1 (MQ=255) cAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCa < 1:1663651/68‑1 (MQ=255) cAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCa < 1:2639431/68‑1 (MQ=255) cAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCa < 1:1246034/68‑1 (MQ=255) cagggcagggAAGCGAGTTACCCCGCCTTGTTCTTGTTTGGCACGACGGAATATCataag > 1:2002114/1‑58 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTg > 1:3238430/1‑36 (MQ=37) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:985512/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:2553230/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:300396/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:2557373/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:505933/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:3133050/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:395133/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:663380/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:2256391/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:2239245/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:2217730/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:1670859/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:3429309/1‑69 (MQ=255) cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:1438798/1‑69 (MQ=255) agcagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAat < 1:389966/69‑1 (MQ=255) agcagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAat < 1:2867784/69‑1 (MQ=255) agcagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAat < 1:276570/69‑1 (MQ=255) agcagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAat < 1:2349422/69‑1 (MQ=255) cagGGAAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:2711718/40‑1 (MQ=37) cagGGAAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:982251/40‑1 (MQ=37) cagGGAAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:1740188/40‑1 (MQ=37) cagGGAAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:794574/40‑1 (MQ=37) cagGGAAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:793462/40‑1 (MQ=37) cagGGAAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:701167/40‑1 (MQ=37) cagGGAAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:2456610/40‑1 (MQ=37) cagGGAAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:1057685/40‑1 (MQ=37) cagGGAAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:1001873/40‑1 (MQ=37) cagGGAAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:2996230/40‑1 (MQ=37) cagGGAAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:59119/40‑1 (MQ=37) cagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGAc > 1:3238188/1‑42 (MQ=255) cagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAatca > 1:157331/1‑69 (MQ=255) cagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAatca > 1:1770503/1‑69 (MQ=255) cagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAatca > 1:3239351/1‑69 (MQ=255) cagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGACAGGCTAatca > 1:322880/1‑69 (MQ=255) agGGTAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:546867/39‑1 (MQ=25) agGGAAGCGAGTTACCACGCCTTGTTCTTGTTTGGcacg < 1:377238/39‑1 (MQ=37) ggAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg < 1:2458481/69‑1 (MQ=255) ggAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg < 1:324001/69‑1 (MQ=255) ggAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg < 1:2661706/69‑1 (MQ=255) ggAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg < 1:2405033/69‑1 (MQ=255) gAAGCGTGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:443248/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:2708161/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:65772/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:530296/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:642834/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:3188176/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:1204387/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:3024688/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:1424792/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:1688739/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:1936409/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:2828677/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:2320139/1‑68 (MQ=255) cGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAg < 1:3186164/69‑1 (MQ=255) cGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAg < 1:3001362/69‑1 (MQ=255) gTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTg < 1:2965201/69‑1 (MQ=255) aCCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGAtt > 1:203628/1‑69 (MQ=255) aCCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGAtt > 1:1878790/1‑69 (MQ=255) aCCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGAtt > 1:1845618/1‑69 (MQ=255) aCCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGAtt > 1:1057318/1‑69 (MQ=255) ccACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCa < 1:1872267/36‑1 (MQ=255) tGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCa < 1:2343717/69‑1 (MQ=255) tGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCa < 1:2391600/69‑1 (MQ=255) tGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCa < 1:57120/69‑1 (MQ=255) tGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCa < 1:3065525/69‑1 (MQ=255) | CCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCA > minE/105071‑105188 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |