| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,302,292 | A→G | 43.5% | S263S (AGT→AGC) | dacD ← | D‑alanyl‑D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,302,292 | 0 | A | G | 43.5% | 6.4 / 33.7 | 46 | S263S (AGT→AGC) | dacD | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base A (6/20); new base G (5/15); total (11/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
GTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTA > minE/1302238‑1302358 | gTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt < 1:2298621/69‑1 (MQ=255)gTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt < 1:3358929/69‑1 (MQ=255)gTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt < 1:1135112/69‑1 (MQ=255)gTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt < 1:1210140/69‑1 (MQ=255)gTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt < 1:1992600/69‑1 (MQ=255)gTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt < 1:2625337/69‑1 (MQ=255)gTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt < 1:2518744/69‑1 (MQ=255)gTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt < 1:743353/69‑1 (MQ=255) ttttGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAtt < 1:1171543/69‑1 (MQ=255) ttttGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAtt < 1:81453/69‑1 (MQ=255) ttttGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCAt < 1:1837964/68‑1 (MQ=255) ttgttgCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTAc < 1:2700608/69‑1 (MQ=255) ccAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCGCTGTCAGCCCCCATTACCACTGc > 1:937130/1‑66 (MQ=255) ccAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCGCTGTCAGCCCCCATTACCACTGCa > 1:2331845/1‑67 (MQ=255) ccAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCGCTGTCAGCCCCCATTACCACTGCa > 1:704630/1‑67 (MQ=255) ccAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCGCTGTCAGCCCCCATTACCACTGCa > 1:1369474/1‑67 (MQ=255) ccAACGCAGTAATTTTCTTGCATCTTCCTCACGACCTTTTGCGCTGTCAGCCCCCATTACCACTGCa > 1:1462830/1‑67 (MQ=255) gTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAgacgac < 1:2859586/69‑1 (MQ=255) gTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAgacgac < 1:3208477/69‑1 (MQ=255) gTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAgacgac < 1:885145/69‑1 (MQ=255) aTTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTGAGCCCCCATTACCACTGc > 1:1122633/1‑55 (MQ=255) aTTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGc > 1:1731757/1‑55 (MQ=255) aTTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGc > 1:1621532/1‑55 (MQ=255) aTTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGc > 1:1536639/1‑55 (MQ=255) ttCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcccc < 1:635208/40‑1 (MQ=255) ttCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcccc < 1:2660202/40‑1 (MQ=255) ttCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcccc < 1:2750049/40‑1 (MQ=255) ttCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcccc < 1:2750918/40‑1 (MQ=255) tGCGCTGTCAGCCCGCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:806935/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:96011/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:961975/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:261315/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:3009444/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:2859397/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:1078286/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:2519954/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:2419094/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:2148964/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:1846603/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:150838/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:1359639/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga < 1:1112068/65‑1 (MQ=255) tGCGCTGTCAGCCCCCATTACCACTGCAATGAGACGAAGCTGCCCATCTACAGCCGAAGCAATga < 1:409604/65‑1 (MQ=255) gCACTGTCAGCCCCCATTACCACTGCAATGAGACGCCGCTGCCCATCTACAGCCGAAGCAATGAGAtt > 1:2538360/1‑68 (MQ=255) gCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGAt < 1:3272255/67‑1 (MQ=255) gCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTa > 1:485721/1‑69 (MQ=255) | GTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTA > minE/1302238‑1302358 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |