| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,302,805 | G→A | 58.4% | R92R (CGC→CGT) | dacD ← | D‑alanyl‑D‑alanine carboxypeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,302,805 | 0 | G | A | 58.4% | 36.8 / 80.6 | 108 | R92R (CGC→CGT) | dacD | D‑alanyl‑D‑alanine carboxypeptidase |
| Reads supporting (aligned to +/- strand): ref base G (19/26); new base A (41/22); total (60/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.02e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 113 total aligned reads displayed.
CGCCCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATAA > minE/1302741‑1302871 | cGCCCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCgcgc > 1:1612955/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTg > 1:7140/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTg > 1:2861069/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTg > 1:2201462/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTg > 1:2157544/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTg > 1:1847119/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTg > 1:52326/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTg > 1:1865194/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTg > 1:3091270/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTg > 1:1051135/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTg > 1:1020003/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGt > 1:1906185/1‑67 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGt > 1:2358889/1‑67 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:3081581/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:3168443/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:1929198/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:1949236/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:3097894/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:2025466/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:1534216/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:1018223/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:1467781/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:1070985/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:2512460/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:2761074/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:815699/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:516362/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:532910/1‑68 (MQ=255) tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTg > 1:756496/1‑68 (MQ=255) aaaCATCTGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAat < 1:1507360/69‑1 (MQ=255) aaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAat < 1:1166150/69‑1 (MQ=255) aaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAat < 1:744318/69‑1 (MQ=255) aaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAat < 1:2773233/69‑1 (MQ=255) aaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAat < 1:3327293/69‑1 (MQ=255) aaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAat < 1:2276565/69‑1 (MQ=255) aaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAat < 1:3030750/68‑1 (MQ=255) aaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAat < 1:2494976/68‑1 (MQ=255) aaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAat < 1:27083/68‑1 (MQ=255) gaaCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGt < 1:1545112/68‑1 (MQ=255) gaaCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGt < 1:475226/68‑1 (MQ=255) gaaCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGt < 1:140143/68‑1 (MQ=255) aaCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGt < 1:356334/67‑1 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGccc > 1:3259432/1‑38 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:3034995/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:3297092/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:353362/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:2911754/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:2906355/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:387979/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:493327/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:929615/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:2737739/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:929756/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:1572598/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:1073580/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:2533361/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:2675106/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:1834497/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:1182438/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGt > 1:2107710/1‑62 (MQ=255) aCAAACACCGGATTATCTTTCGCCCACGCATCACGCCCCACGGTGACAATATCGTCTGGCGTaaaa > 1:351581/1‑64 (MQ=255) cAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGAt < 1:1968603/69‑1 (MQ=255) cAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGAt < 1:612563/69‑1 (MQ=255) cAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGAt < 1:3095336/69‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTgg > 1:2586185/1‑54 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACt > 1:779196/1‑69 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACt > 1:1961064/1‑69 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACt > 1:1891834/1‑69 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACt > 1:1869505/1‑69 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACt > 1:836300/1‑69 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:1036666/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:1147038/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:1499204/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:1325374/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:907613/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:1204274/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:1085639/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:7870/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:334913/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:1088311/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:1155526/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:1627614/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:2537478/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:2776396/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:2323352/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:2153435/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:2108072/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:3169538/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:3416928/35‑1 (MQ=255) cacCGGATTATCTTTCGCCCACGCATCACGCCCCa < 1:1773889/35‑1 (MQ=255) gATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGt < 1:1426847/52‑1 (MQ=255) aTCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAgc > 1:3277459/1‑69 (MQ=255) gCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATc < 1:300817/68‑1 (MQ=255) gCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATc < 1:821312/68‑1 (MQ=255) gCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATc < 1:2360729/68‑1 (MQ=255) gCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATc < 1:260965/68‑1 (MQ=255) tCGCGCCCCACGGTGACAATATCGTTTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATaa < 1:2288012/69‑1 (MQ=255) tCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATaa < 1:368124/69‑1 (MQ=255) cgcgCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATaa < 1:2977341/68‑1 (MQ=255) gcgcCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATc < 1:1803630/45‑1 (MQ=255) | CGCCCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATAA > minE/1302741‑1302871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |