| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,307,479 | A→T | 25.2% | T39T (ACT→ACA) | yeeF ← | predicted amino‑acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,307,479 | 0 | A | T | 25.2% | 157.8 / 54.6 | 103 | T39T (ACT→ACA) | yeeF | predicted amino‑acid transporter |
| Reads supporting (aligned to +/- strand): ref base A (46/31); new base T (8/18); total (54/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.30e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
AATGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACTAACGTAAGCG > minE/1307420‑1307546 | aatGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAAc > 1:2225833/1‑69 (MQ=255)aatGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAAc > 1:2255075/1‑69 (MQ=255)aatGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAAc > 1:2923834/1‑69 (MQ=255)aatGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAAc > 1:468249/1‑69 (MQ=255)aatGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAAc > 1:3245022/1‑69 (MQ=255)aatGCGAACGCATAGGCTGTCGGCACATGACAATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAAc > 1:3006226/1‑69 (MQ=255) ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:921875/65‑1 (MQ=255) ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:2351115/65‑1 (MQ=255) ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:2410855/65‑1 (MQ=255) ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:2968281/65‑1 (MQ=255) ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:1783177/65‑1 (MQ=255) ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:3097665/65‑1 (MQ=255) ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:1496004/65‑1 (MQ=255) ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:901511/65‑1 (MQ=255) ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:786572/65‑1 (MQ=255) ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:1429440/65‑1 (MQ=255) ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:1949770/1‑69 (MQ=255) ggCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:2900921/1‑69 (MQ=255) cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:2801190/69‑1 (MQ=255) cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:230278/69‑1 (MQ=255) cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:2672928/69‑1 (MQ=255) cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:1803583/69‑1 (MQ=255) cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:522120/69‑1 (MQ=255) cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:502556/69‑1 (MQ=255) cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:158885/69‑1 (MQ=255) cTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:3192961/69‑1 (MQ=255) cTGTCGGCACATGACCATCCGTGAGGCATGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa < 1:273480/69‑1 (MQ=255) gTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCg < 1:2146470/61‑1 (MQ=255) gTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCata < 1:826282/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:689245/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:617620/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:3389029/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:3031242/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:2491353/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:2335029/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:1146408/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:2196574/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:1783170/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:1420904/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:1307545/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:1248460/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:2063548/69‑1 (MQ=255) cacaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:2126599/69‑1 (MQ=255) acaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:2326851/68‑1 (MQ=255) acaTGACCATCCGTGAGGCCTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGc < 1:1594175/68‑1 (MQ=255) caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:2382222/1‑58 (MQ=255) caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:2073247/1‑58 (MQ=255) caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:1767450/1‑58 (MQ=255) caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:2595928/1‑58 (MQ=255) caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:743491/1‑58 (MQ=255) caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:2137171/1‑58 (MQ=255) caTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTg > 1:480122/1‑58 (MQ=255) aTGACCATCCGTGAGGCCTGGAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:431811/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAGAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:1873203/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCaa > 1:1530308/1‑68 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:2141461/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:3067466/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:1587005/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:149227/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:1460015/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:63199/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:943732/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:945860/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:2767078/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:1784245/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:1880109/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:245417/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:2966399/1‑69 (MQ=255) aTGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAg > 1:187048/1‑69 (MQ=255) aTGACAATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAg > 1:2056712/1‑64 (MQ=255) tCCGTGAGGCCTGAGACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:3076209/1‑52 (MQ=255) tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGc > 1:381954/1‑51 (MQ=255) tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:1894492/1‑52 (MQ=255) tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:3212540/1‑52 (MQ=255) tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:3235876/1‑52 (MQ=255) tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:918384/1‑52 (MQ=255) tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:2002942/1‑52 (MQ=255) tCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCa > 1:868918/1‑52 (MQ=255) cGTGAGGCCTGAAACGATCCCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCcatcat > 1:883259/1‑69 (MQ=255) cGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCcatcat > 1:2861686/1‑69 (MQ=255) cGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCcatcat > 1:1952544/1‑69 (MQ=255) cGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCcatca > 1:688854/1‑68 (MQ=255) cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:947922/1‑68 (MQ=255) cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:1004414/1‑68 (MQ=255) cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:2421852/1‑68 (MQ=255) cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:724133/1‑68 (MQ=255) cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:2993234/1‑68 (MQ=255) cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:1443504/1‑68 (MQ=255) cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:2972846/1‑68 (MQ=255) cTGAAACGATACCAAATGTATCAAACAGCGTCATCGGCTGCATATAGGCTAGACCCATCATTACAAcc > 1:2983064/1‑68 (MQ=255) aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:661373/69‑1 (MQ=255) aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:763755/69‑1 (MQ=255) aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:2863784/69‑1 (MQ=255) aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:3384804/69‑1 (MQ=255) aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:3347788/69‑1 (MQ=255) aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:1531849/69‑1 (MQ=255) aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:297996/69‑1 (MQ=255) aCGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATAATTACAACCGGAACt < 1:1479152/69‑1 (MQ=255) aCGATACCAAAAGTATCAAACAGCGGCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACt < 1:344161/69‑1 (MQ=255) aaGTATCAAACAGCGTCATCGCCTGCATATAGGCAAGACCCATCATTACAACCGGAACTAACGTAAGCg < 1:1415702/69‑1 (MQ=255) | AATGCGAACGCATAGGCTGTCGGCACATGACCATCCGTGAGGCCTGAAACGATACCAAAAGTATCAAACAGCGTCATCGGCTGCATATAGGCAAGACCCATCATTACAACCGGAACTAACGTAAGCG > minE/1307420‑1307546 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |