| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,308,306 | T→C | 37.7% | S162S (TCA→TCG) | yeeY ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,308,306 | 0 | T | C | 37.7% | 45.8 / 59.0 | 90 | S162S (TCA→TCG) | yeeY | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (30/26); new base C (27/7); total (57/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01 | |||||||||||
GCTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACAATAATATCCGCTCG > minE/1308253‑1308368 | gCTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:2402050/1‑69 (MQ=255)gCTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:2000533/1‑69 (MQ=255)gCTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:1041707/1‑69 (MQ=255)gCTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:940436/1‑69 (MQ=255)gCTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:1463501/1‑69 (MQ=255)gCTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:1379094/1‑69 (MQ=255)gCTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:1189699/1‑69 (MQ=255)gCTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:1236486/1‑69 (MQ=255)gCTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:1247653/1‑69 (MQ=255)gCTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:1375107/1‑69 (MQ=255)gCTAACGGATGATGAG‑‑GCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGt > 1:2495465/1‑52 (MQ=255) cTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCAc > 1:3010244/1‑69 (MQ=255) ggATGATGAGGCGCGACCGCAAATACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGaa < 1:1181745/69‑1 (MQ=255) tgatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGAt < 1:1468636/69‑1 (MQ=255) tgatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGAt < 1:880363/69‑1 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTc > 1:3100873/1‑58 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:1881504/1‑59 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:2435228/1‑59 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:786598/1‑59 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:2197178/1‑59 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:496961/1‑59 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:815911/1‑59 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:1310769/1‑59 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:468626/1‑59 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:1561323/1‑59 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:3036826/1‑59 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCa > 1:1306348/1‑59 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGaga > 1:2127841/1‑46 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:3399835/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:2639174/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:3356193/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:2788440/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:540796/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:2851266/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:660570/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:661270/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:2717239/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:2404330/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:2119537/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:1531119/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:1095280/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:1106925/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:1127922/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:1359373/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:1379620/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:1507939/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:724142/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:2000392/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:810039/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:2124485/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:2226504/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:773706/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAACCGCGAGAAAc > 1:2299343/1‑51 (MQ=255) gatgaGGCGCGACCGCAAAGACTTGTTCAAGATCGCCCAAACGCGAGAAAc > 1:3209374/1‑51 (MQ=255) gaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGAtggt < 1:1178178/68‑1 (MQ=255) gaGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGAtggt > 1:3026891/1‑68 (MQ=255) gACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATg < 1:854233/69‑1 (MQ=255) gACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATg < 1:906192/69‑1 (MQ=255) gACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATg < 1:3231496/69‑1 (MQ=255) gACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATg < 1:942739/69‑1 (MQ=255) gACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATg < 1:2334534/69‑1 (MQ=255) gACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATg < 1:1943310/69‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:2978024/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:2263825/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:2065072/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:2467398/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:58725/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:2578877/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:2983452/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:2586172/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:434004/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:2599843/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:270776/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:3353250/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:2716108/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:2728696/68‑1 (MQ=255) aGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:2782566/68‑1 (MQ=255) gATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACaataat < 1:1877212/67‑1 (MQ=255) cGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACAataata > 1:3412939/1‑65 (MQ=255) cGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACAATAATATcc > 1:2912531/1‑68 (MQ=255) cGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACAATAATATCCg > 1:906159/1‑69 (MQ=255) cGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACAATAATATCCg > 1:1167166/1‑69 (MQ=255) cGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACAATAATATCCg > 1:2584008/1‑69 (MQ=255) cGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACAATAATATCCg > 1:2731510/1‑69 (MQ=255) cAACCGCGAGAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACGATAATATCCGCTCg < 1:2287780/69‑1 (MQ=255) cAACCGCGAGAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACGATAATATCCGCTCg < 1:3113997/69‑1 (MQ=255) cAACCGCGAGAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACGATAATATCCGCTCg < 1:1615189/69‑1 (MQ=255) acccGCGAGAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACGATAATATCCGCTCg < 1:1641659/66‑1 (MQ=255) aaCCGCGAGAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACGATAATATCCGCTCg < 1:2461478/68‑1 (MQ=255) aaCCGCGAGAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACGATAATATCCGCTCg < 1:350470/68‑1 (MQ=255) aaCCGCGAGAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACGATAATATCCGCTCg < 1:1159347/68‑1 (MQ=255) | GCTAACGGATGATGAGGCGCGACCGCAAAGACTTGTTCCAGATCGCCCAACCGTGAAAAACCAAACTCACTGGAAGATGGTGGCTCATGCATCGCGCCAACAATAATATCCGCTCG > minE/1308253‑1308368 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |