| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,309,899 | G→A | 34.4% | R31R (CGC→CGT) | yefM ← | antitoxin of the YoeB‑YefM toxin‑antitoxin system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,309,899 | 0 | G | A | 34.4% | 84.7 / 61.0 | 110 | R31R (CGC→CGT) | yefM | antitoxin of the YoeB‑YefM toxin‑antitoxin system |
| Reads supporting (aligned to +/- strand): ref base G (39/33); new base A (15/23); total (54/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.92e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
AAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCC > minE/1309835‑1309950 | aaGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCg > 1:2386704/1‑67 (MQ=255)aaGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGa > 1:348990/1‑68 (MQ=255)aaGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGa > 1:695816/1‑68 (MQ=255)aaGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAg < 1:3022147/69‑1 (MQ=255)aaGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAg < 1:2599423/69‑1 (MQ=255)aaGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAg < 1:2842412/69‑1 (MQ=255)aaGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAg < 1:1315114/69‑1 (MQ=255)aaGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAg < 1:1276272/69‑1 (MQ=255) aGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAg < 1:96555/68‑1 (MQ=255) tctcTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAg < 1:208149/63‑1 (MQ=255) tctTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGt > 1:2749678/1‑62 (MQ=255) tctTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAgg > 1:479931/1‑69 (MQ=255) ccAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCg < 1:1453520/55‑1 (MQ=255) gTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:831147/69‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc < 1:2020499/69‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc < 1:513526/69‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc < 1:358893/69‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc < 1:2778940/69‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc < 1:1390640/69‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc < 1:1258091/69‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc < 1:248848/69‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:2481596/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:2117675/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:2360386/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:1993968/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:2486720/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:1975103/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:2856189/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:2896075/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:1791315/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:3033897/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:1708820/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:3136299/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:1639345/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:431734/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:491259/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:1383413/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:618697/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:961112/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:1222331/54‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:1132205/54‑1 (MQ=255) tcttcGAGTGACATCAGAACACAAGACTCTCCATTCTGACGAGTAATGAGGAt < 1:2520402/53‑1 (MQ=255) cttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAgg > 1:2453772/1‑50 (MQ=255) cttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAgg > 1:601417/1‑50 (MQ=255) cttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAgg > 1:582899/1‑50 (MQ=255) ttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGAt < 1:3110183/51‑1 (MQ=255) tGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:3332261/54‑1 (MQ=255) tGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:1739227/54‑1 (MQ=255) gACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:2430894/53‑1 (MQ=255) gACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtc > 1:1588838/1‑69 (MQ=255) aTCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcat < 1:300450/68‑1 (MQ=255) aTCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcat < 1:2830946/68‑1 (MQ=255) aTCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcat < 1:2116336/68‑1 (MQ=255) aTCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcat < 1:3043489/68‑1 (MQ=255) cAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:2311045/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:480263/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:51856/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACTAGTAATGAGGa > 1:892967/1‑38 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGATCGGGGCATGATCTTCAACGGCTTtcatca > 1:2607878/1‑68 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:1459608/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:140894/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:1700828/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:618862/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:701266/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:109188/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:244006/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:1024826/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:2161010/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:2472689/1‑69 (MQ=255) cAGAACACAAGCCTCTCCATTCTGACGAGTAATGAGGATCGGGGCATGATCTTCAACGGCTTTcatcat > 1:1960799/1‑69 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCTGGGCATGATCTTCAACGGCTTTCATCAtt > 1:2380576/1‑69 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:955678/1‑53 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:3147460/1‑53 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:3303893/1‑53 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:2713948/1‑53 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:2695565/1‑53 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:719827/1‑53 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:2407530/1‑53 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:129262/1‑53 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc > 1:2492800/1‑53 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAtt > 1:1224506/1‑69 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAtt > 1:1233976/1‑69 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAtt > 1:2390317/1‑69 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAtt > 1:140158/1‑69 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAtt > 1:2620671/1‑69 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAtt > 1:388725/1‑69 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAtt > 1:1792158/1‑69 (MQ=255) cacaAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:2944370/43‑1 (MQ=255) cacaAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:643581/43‑1 (MQ=255) cacaAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:1513009/43‑1 (MQ=255) cacaAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:850317/43‑1 (MQ=255) cacaAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:893917/43‑1 (MQ=255) cacaAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgttg > 1:1350961/1‑69 (MQ=255) cacaAGCCTCTCCATTCTGGCGAGAAATAAGGATCGGGGCATg < 1:139670/43‑1 (MQ=255) caAGGCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg > 1:1854201/1‑64 (MQ=255) caAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATg < 1:3046661/41‑1 (MQ=255) caAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGcc > 1:2567617/1‑69 (MQ=255) caAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGcc > 1:3360290/1‑69 (MQ=255) caAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGcc > 1:212194/1‑69 (MQ=255) caAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGc > 1:2662316/1‑68 (MQ=255) | AAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCC > minE/1309835‑1309950 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |