| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,310,943 | C→G | 61.8% | A158A (GCC→GCG) | hisG → | ATP phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,310,943 | 0 | C | G | 61.8% | 50.8 / 68.3 | 76 | A158A (GCC→GCG) | hisG | ATP phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (19/10); new base G (16/31); total (35/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.67e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01 | |||||||||||
CGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAC > minE/1310878‑1310996 | cgatATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGcccc > 1:102532/4‑68 (MQ=255)cGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcg > 1:1017028/1‑69 (MQ=255)cGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcg > 1:3044762/1‑69 (MQ=255)cGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcg > 1:2299798/1‑69 (MQ=255)cGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcg > 1:3138780/1‑69 (MQ=255)cGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcg > 1:3186077/1‑69 (MQ=255)cGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcg > 1:1219783/1‑69 (MQ=255)cGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcg > 1:1244647/1‑69 (MQ=255)cGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcg > 1:2173536/1‑69 (MQ=255) gTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcgc > 1:3380619/1‑69 (MQ=255) gTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcgc > 1:1714408/1‑69 (MQ=255) aTCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTg > 1:2753115/1‑69 (MQ=255) aTCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTg > 1:2806068/1‑69 (MQ=255) aTCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTg > 1:386483/1‑69 (MQ=255) ccAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCgg < 1:1492785/66‑1 (MQ=255) ccAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCgg < 1:1907997/66‑1 (MQ=255) ccAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCgg < 1:2064590/66‑1 (MQ=255) ccAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCgg < 1:2253964/66‑1 (MQ=255) ccAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCgg < 1:3318877/66‑1 (MQ=255) ccAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCgg < 1:1373576/66‑1 (MQ=255) tctTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTGGCGGATGCGAtt > 1:3269528/1‑69 (MQ=255) tctTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTGGCGGATGCGAtt > 1:2363788/1‑69 (MQ=255) tctTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTGGCGGATGCGAtt > 1:693547/1‑69 (MQ=255) tctTTTAAATCCTGCTTAATGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTGGCGGATGCGAtt > 1:280881/1‑69 (MQ=255) tctTTTAAATCATGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTg > 1:768476/1‑49 (MQ=255) ttAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTGGCGGATGCGATTTGCg > 1:2176903/1‑69 (MQ=255) aaTCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTGGCGGATGCGATTTGCg < 1:2166224/66‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:539789/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:397844/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:2949472/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:3005525/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:353669/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:3006683/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:95174/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:3103589/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:3404098/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:656858/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:978386/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:3245139/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:573940/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:3296532/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:2591655/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:1644253/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:1764379/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:1822490/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:1194841/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:1100018/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:2201315/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:2248136/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:2248473/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:2260103/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:2266845/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:2352329/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:1099197/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:2352988/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:2471014/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:2504817/60‑1 (MQ=255) tGAACGGTTCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTgg < 1:2584961/60‑1 (MQ=255) cGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGc < 1:2669240/69‑1 (MQ=255) cGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGc < 1:1984866/69‑1 (MQ=255) ttCTGTCTAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCAcc > 1:3217814/1‑61 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:791238/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:823209/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:1126395/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:639133/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:1643613/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:1068231/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:520168/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:2048130/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:2076221/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:214077/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:3163798/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:2337303/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:3015913/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAc > 1:2391063/1‑69 (MQ=255) ttCTGTCGAAGTTGCGCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCAACCGGTGCCAc > 1:769078/1‑69 (MQ=255) tCGCCCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGc < 1:3330803/55‑1 (MQ=255) | CGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTGGCGGATGCGATTTGCGATCTGGTTTCCACCGGTGCCAC > minE/1310878‑1310996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |