| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,312,382 | T→C | 46.6% | R336R (CGT→CGC) | hisD → | bifunctional histidinal dehydrogenase and histidinol dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,312,382 | 0 | T | C | 46.6% | 4.7 / 61.6 | 73 | R336R (CGT→CGC) | hisD | bifunctional histidinal dehydrogenase and histidinol dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (25/14); new base C (11/23); total (36/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.80e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
TGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAAT > minE/1312320‑1312443 | tGCGTGGATATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTg < 1:2139071/69‑1 (MQ=255)tGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTg < 1:572413/69‑1 (MQ=255)tGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTg < 1:2217879/69‑1 (MQ=255)tGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTg < 1:2149720/69‑1 (MQ=255)tGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTg < 1:923571/69‑1 (MQ=255)tGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTg < 1:2577119/69‑1 (MQ=255)tGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTg < 1:1839544/69‑1 (MQ=255)tGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTg < 1:1507161/69‑1 (MQ=255)tGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTg < 1:433166/69‑1 (MQ=255)tGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTg < 1:3396548/69‑1 (MQ=255) ttgagaTCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCTTGAACTGTTc < 1:732953/66‑1 (MQ=255) tGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTc < 1:1401733/68‑1 (MQ=255) tGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTc < 1:3119987/68‑1 (MQ=255) tGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTc < 1:1749919/68‑1 (MQ=255) tGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTc < 1:781036/68‑1 (MQ=255) ggAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTc < 1:1553831/67‑1 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:720693/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:2485731/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:530505/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:385561/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:373672/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:2765312/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:2786834/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:2831902/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:2917882/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:77656/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:3172455/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:1303589/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:1310129/1‑59 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCAt > 1:311046/1‑69 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAAc > 1:2470381/1‑56 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:1383171/1‑69 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:3094935/1‑69 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:3004423/1‑69 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:1588603/1‑69 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:1711087/1‑69 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:3159761/1‑69 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:1424219/1‑69 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:261642/1‑69 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:2500662/1‑69 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:1219115/1‑69 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCAGTGAACTGGTCGATAGCAt > 1:1701413/1‑69 (MQ=255) ctcCAACCAGTACGGCCCGGAGCACCTAATCATTCAGACCCGCAACGCCCGTGAACTgg > 1:2242784/1‑59 (MQ=255) aCCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATc < 1:465296/65‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:3085517/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:3253324/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:474404/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:1522628/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:109831/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:2474099/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:1921264/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:2129595/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:2621967/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:2099685/59‑1 (MQ=255) aCGGCCCGGAGGACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:818062/57‑1 (MQ=255) ggCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTg < 1:2794614/45‑1 (MQ=255) gCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:3259077/54‑1 (MQ=39) cGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCgg < 1:2191234/69‑1 (MQ=255) gCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGt < 1:711575/66‑1 (MQ=255) gCACCTGATCATTCAGACCCGCAACGCCCGTAAACTGGTCGATAGCATCACCAGCGCCGGTTCGGt < 1:706480/66‑1 (MQ=255) cACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGt < 1:2707932/65‑1 (MQ=255) gATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCgg < 1:1922651/59‑1 (MQ=255) aTCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGt > 1:944814/1‑69 (MQ=255) aTCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGt > 1:1235110/1‑69 (MQ=255) aTCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGt > 1:823329/1‑69 (MQ=255) aTTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGAc > 1:1187069/1‑69 (MQ=255) aTTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGAc > 1:3349730/1‑69 (MQ=255) aTTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGAc > 1:2954298/1‑69 (MQ=255) aTTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGAc > 1:2179190/1‑69 (MQ=255) aTTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGAc > 1:1366784/1‑69 (MQ=255) cAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGa < 1:2692190/69‑1 (MQ=255) cAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGa < 1:2138563/69‑1 (MQ=255) aCGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAAt > 1:2777495/1‑69 (MQ=255) | TGCGTGGAGATCTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAAT > minE/1312320‑1312443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |