| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,312,395 | A→G | 48.9% | S341G (AGC→GGC) | hisD → | bifunctional histidinal dehydrogenase and histidinol dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,312,395 | 0 | A | G | 48.9% | ‑5.9 / 39.5 | 45 | S341G (AGC→GGC) | hisD | bifunctional histidinal dehydrogenase and histidinol dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (11/12); new base G (10/12); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGTGATTACGCCTCC > minE/1312331‑1312463 | ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCAt > 1:311046/1‑69 (MQ=255)ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:1219115/1‑69 (MQ=255)ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:3004423/1‑69 (MQ=255)ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:3094935/1‑69 (MQ=255)ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:1383171/1‑69 (MQ=255)ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:1424219/1‑69 (MQ=255)ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:3159761/1‑69 (MQ=255)ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:1588603/1‑69 (MQ=255)ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:2500662/1‑69 (MQ=255)ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:1711087/1‑69 (MQ=255)ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCAt > 1:261642/1‑69 (MQ=255)ctcCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCAGTGAACTGGTCGATAGCAt > 1:1701413/1‑69 (MQ=255) aCCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATc < 1:465296/65‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:3253324/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:2474099/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:2621967/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:2099685/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:3085517/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:2129595/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:1921264/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:474404/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:1522628/59‑1 (MQ=255) gTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:109831/59‑1 (MQ=255) aCGGCCCGGAGGACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:818062/57‑1 (MQ=255) gCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGCGAACTGGTCGATGGCa < 1:3259077/54‑1 (MQ=39) cGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCgg < 1:2191234/69‑1 (MQ=255) gCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGt < 1:711575/66‑1 (MQ=255) gCACCTGATCATTCAGACCCGCAACGCCCGTAAACTGGTCGATAGCATCACCAGCGCCGGTTCGGt < 1:706480/66‑1 (MQ=255) cACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGt < 1:2707932/65‑1 (MQ=255) gATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCgg < 1:1922651/59‑1 (MQ=255) aTCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGt > 1:944814/1‑69 (MQ=255) aTCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGt > 1:823329/1‑69 (MQ=255) aTCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGt > 1:1235110/1‑69 (MQ=255) aTTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGAc > 1:2954298/1‑69 (MQ=255) aTTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGAc > 1:2179190/1‑69 (MQ=255) aTTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGAc > 1:3349730/1‑69 (MQ=255) aTTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGAc > 1:1366784/1‑69 (MQ=255) aTTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGAc > 1:1187069/1‑69 (MQ=255) cAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGa < 1:2692190/69‑1 (MQ=255) cAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGa < 1:2138563/69‑1 (MQ=255) aCGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAAt > 1:2777495/1‑69 (MQ=255) ggTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGt < 1:472437/40‑1 (MQ=255) ggTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGt < 1:1645654/40‑1 (MQ=255) ggTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGt < 1:1307343/40‑1 (MQ=255) ggTCGATAGCATCACCAGCGCCGGTTCGGTATTTCCTGGt < 1:905311/40‑1 (MQ=255) aGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGt < 1:1518099/57‑1 (MQ=255) aGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGTGATTACGCCTcc > 1:2846417/1‑69 (MQ=255) | CTCCAACCAGTACGGCCCGGAGCACCTGATCATTCAGACCCGCAACGCCCGTGAACTGGTCGATAGCATCACCAGCGCCGGTTCGGTATTTCTTGGTGACTGGTCACCGGAATCGGCAGGTGATTACGCCTCC > minE/1312331‑1312463 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |