| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,314,240 | T→A | 28.7% | R165R (CGT→CGA) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,314,240 | 0 | T | A | 28.7% | 175.3 / 81.0 | 132 | R165R (CGT→CGA) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (45/49); new base A (21/17); total (66/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 132 total aligned reads displayed.
ACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCT > minE/1314175‑1314302 | aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:1943170/1‑69 (MQ=255)aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:1115276/1‑69 (MQ=255)aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:1670300/1‑69 (MQ=255)aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:2726652/1‑69 (MQ=255)aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:3372931/1‑69 (MQ=255)aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:3149141/1‑69 (MQ=255)aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:1366870/1‑69 (MQ=255)aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:2630526/1‑69 (MQ=255)aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:87529/1‑69 (MQ=255)aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAc > 1:2622425/1‑69 (MQ=255) ggTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:1811112/69‑1 (MQ=255) ggTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:1693282/69‑1 (MQ=255) ggTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:228502/69‑1 (MQ=255) ggTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:2551961/69‑1 (MQ=255) ggTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:1422218/69‑1 (MQ=255) ggTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:974933/69‑1 (MQ=255) ggTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:2751777/69‑1 (MQ=255) ggTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:1057508/69‑1 (MQ=255) tACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACg > 1:679170/1‑69 (MQ=255) gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:235518/68‑1 (MQ=255) gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:2920568/68‑1 (MQ=255) gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:2693717/68‑1 (MQ=255) gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:2760401/68‑1 (MQ=255) gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:2820928/68‑1 (MQ=255) gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:968678/68‑1 (MQ=255) gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:1773522/68‑1 (MQ=255) gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:731789/68‑1 (MQ=255) gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:823737/68‑1 (MQ=255) gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:827846/68‑1 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:863382/67‑1 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2015430/67‑1 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2393262/67‑1 (MQ=255) aCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:1487137/47‑1 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:1383668/46‑1 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:3071039/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1351958/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2531587/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2457868/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2372258/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:842111/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1294335/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:320169/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2200629/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1176008/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:405993/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1004565/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:675360/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1850906/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1767672/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1808150/3‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTATGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:2625292/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2680487/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:3001556/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:3340604/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:443951/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:512660/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:1001104/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2143869/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:506822/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:1717406/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:844075/1‑68 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGTTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2157673/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2295515/41‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:836983/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:463524/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:392819/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:384040/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:36162/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3427525/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1695059/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3119884/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:843167/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2890087/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1514384/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1401765/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2827078/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2783626/1‑69 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1570672/1‑69 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1550899/1‑69 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:2142160/69‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:899019/69‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:220111/69‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:3198566/69‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTCACGTTc < 1:435799/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATGCCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:1304822/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:2858445/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:2886370/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:2246130/69‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAATAGACGCAAATTGACGTCCAGGTGTGGCt < 1:978431/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1575179/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:3122365/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1542223/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2255749/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2946927/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2791658/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2865435/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:596153/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2000525/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2014797/66‑1 (MQ=255) aaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1460387/66‑1 (MQ=255) | ACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCT > minE/1314175‑1314302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |