| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,314,252 | T→C | 42.5% | A169A (GCT→GCC) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,314,252 | 0 | T | C | 42.5% | 26.4 / 72.2 | 94 | A169A (GCT→GCC) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (28/26); new base C (21/19); total (49/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 108 total aligned reads displayed.
GCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCT > minE/1314185‑1314302 | gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:968678/68‑1 (MQ=255)gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:235518/68‑1 (MQ=255)gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:1773522/68‑1 (MQ=255)gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:2647601/68‑1 (MQ=255)gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:2693717/68‑1 (MQ=255)gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:2760401/68‑1 (MQ=255)gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:2820928/68‑1 (MQ=255)gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:2920568/68‑1 (MQ=255)gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:827846/68‑1 (MQ=255)gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:837632/68‑1 (MQ=255)gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:731789/68‑1 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2015430/67‑1 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:85617/67‑1 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2393262/67‑1 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:863382/67‑1 (MQ=255) aCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:1487137/47‑1 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt < 1:1383668/46‑1 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:25853/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2200629/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1004565/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2372258/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2428536/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2457868/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2531587/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:842111/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:3071039/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:320169/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:675360/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2022661/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1351958/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1294335/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1767672/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1808150/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1850906/3‑68 (MQ=255) cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1176008/3‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTATGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:2625292/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:1161370/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:512660/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:1001104/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:802545/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2811485/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:3340604/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2143869/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:443951/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:1656977/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2680487/42‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:1546485/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:506822/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:467611/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:3278024/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:1717406/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:844075/1‑68 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGTTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2157673/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:463524/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:392819/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3119884/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1514384/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:384040/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:36162/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3427525/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2890087/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1401765/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:285864/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2827078/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2783626/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:836983/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1695059/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:843167/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2248628/1‑69 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:645457/1‑69 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1550899/1‑69 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1570672/1‑69 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:2177280/1‑69 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:899019/69‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:2142160/69‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:220111/69‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:3198566/69‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTCACGTTc < 1:435799/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATGCCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:1304822/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:2246130/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:2858445/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:2886370/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:1397864/69‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCTCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1715190/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAATAGACGCAAATTGACGTCCAGGTGTGGCt < 1:978431/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2865435/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:596153/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2791658/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1575179/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2946927/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:3038616/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2000525/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2255749/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:3122365/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2014797/66‑1 (MQ=255) aaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1460387/66‑1 (MQ=255) aaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:914324/66‑1 (MQ=255) aaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:429962/66‑1 (MQ=255) gTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2562875/58‑1 (MQ=255) aCGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:808087/55‑1 (MQ=255) | GCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCT > minE/1314185‑1314302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |