| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,314,264 | T→C | 50.6% | R173R (CGT→CGC) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,314,264 | 0 | T | C | 50.6% | ‑2.2 / 71.5 | 79 | R173R (CGT→CGC) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (29/10); new base C (21/19); total (50/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01 | |||||||||||
CAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAAC > minE/1314212‑1314330 | cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2531587/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1767672/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1808150/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1850906/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2022661/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2200629/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2372258/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2428536/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2457868/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1176008/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:25853/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:2756915/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:3071039/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:320169/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:405993/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:675360/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:842111/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:993568/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1004565/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1294335/3‑68 (MQ=255)cgaTGATTGGTGAGCAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACg > 1:1351958/3‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTATGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:2625292/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:844075/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:506822/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:467611/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:1717406/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:1546485/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGa > 1:3278024/1‑68 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGTTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2157673/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2827078/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2890087/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:285864/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1401765/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3119884/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3427525/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:36162/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:384040/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:392819/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:463524/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:836983/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:843167/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2783626/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2248628/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1695059/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1514384/1‑69 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:2177280/1‑69 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1550899/1‑69 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1570672/1‑69 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:645457/1‑69 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:2142160/69‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:3198566/69‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:220111/69‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:899019/69‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTCACGTTc < 1:435799/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATGCCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:1304822/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:2886370/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:1397864/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:2858445/69‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg < 1:2246130/69‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCTCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1715190/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAATAGACGCAAATTGACGTCCAGGTGTGGCt < 1:978431/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2865435/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2791658/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1993443/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2000525/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2014797/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2255749/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:596153/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1575179/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2946927/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:3038616/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1542223/66‑1 (MQ=255) taaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:3122365/66‑1 (MQ=255) aaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:1460387/66‑1 (MQ=255) aaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:914324/66‑1 (MQ=255) aaaCGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:429962/66‑1 (MQ=255) gTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:2562875/58‑1 (MQ=255) aCGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCt < 1:808087/55‑1 (MQ=255) cGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAac > 1:1407849/1‑69 (MQ=255) | CAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGCAAGATTAAC > minE/1314212‑1314330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |