| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,314,660 | C→T | 24.6% | L305L (CTC→CTT) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,314,660 | 0 | C | T | 24.6% | 123.1 / 32.6 | 97 | L305L (CTC→CTT) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (47/26); new base T (11/13); total (58/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01 | |||||||||||
GAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGATCATC > minE/1314595‑1314715 | gAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCa < 1:310515/69‑1 (MQ=255) tttACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATac > 1:701701/1‑69 (MQ=255) tttACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATac > 1:2292637/1‑69 (MQ=255) ttaacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATac > 1:1461699/4‑69 (MQ=255) tacctaccAGCGCGTGGGGGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:1295359/1‑69 (MQ=255) tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGctct < 1:346804/62‑1 (MQ=255) tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2325396/1‑69 (MQ=255) tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:1906631/1‑69 (MQ=255) tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2100395/1‑69 (MQ=255) tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:1411684/1‑69 (MQ=255) tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2115493/1‑69 (MQ=255) tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2287082/1‑69 (MQ=255) tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2390391/1‑69 (MQ=255) tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2467964/1‑69 (MQ=255) tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac < 1:2519250/69‑1 (MQ=255) tacctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATacac > 1:2602999/1‑69 (MQ=255) cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:1020409/68‑1 (MQ=255) cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:2630644/68‑1 (MQ=255) cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:1861729/68‑1 (MQ=255) cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:3046647/68‑1 (MQ=255) cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:16866/68‑1 (MQ=255) cctaccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:1613683/68‑1 (MQ=255) taccAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCAt > 1:1325888/1‑68 (MQ=255) taccAGCGCGTGGGCGATCTCAGCACCGAAAAGAACGAGCACTTCTTCCGATCGCTCTCATACACCATg > 1:1980713/1‑69 (MQ=255) accAGCGCTTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:2906739/1‑69 (MQ=255) accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:1631995/1‑69 (MQ=255) accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:107856/1‑69 (MQ=255) accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:23920/1‑69 (MQ=255) accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:81620/1‑69 (MQ=255) accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:3279815/1‑69 (MQ=255) accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:2993358/1‑69 (MQ=255) accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:678700/1‑69 (MQ=255) accAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATgg > 1:1728738/1‑69 (MQ=255) aGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACAcc < 1:2092041/62‑1 (MQ=255) aGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGc > 1:460720/1‑68 (MQ=255) aGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGc > 1:252309/1‑68 (MQ=255) aGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGc > 1:1725017/1‑68 (MQ=255) tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACg > 1:2926639/1‑68 (MQ=255) tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:2428061/1‑69 (MQ=255) tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:2829735/1‑69 (MQ=255) tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:3307337/1‑69 (MQ=255) tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:2327383/1‑69 (MQ=255) tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:953520/1‑69 (MQ=255) tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:718420/1‑69 (MQ=255) tGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGc > 1:1380635/1‑69 (MQ=255) ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:1010773/59‑1 (MQ=255) ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:3382371/59‑1 (MQ=255) ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:2337218/59‑1 (MQ=255) ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:70166/59‑1 (MQ=255) ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:855511/59‑1 (MQ=255) ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:1491344/59‑1 (MQ=255) ggCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:186985/59‑1 (MQ=255) gCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATggg < 1:952533/58‑1 (MQ=255) aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:2891237/69‑1 (MQ=255) aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:3222942/69‑1 (MQ=255) aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:1026886/69‑1 (MQ=255) aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:917852/69‑1 (MQ=255) aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:1326324/69‑1 (MQ=255) aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:2289844/69‑1 (MQ=255) aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:187208/69‑1 (MQ=255) aTCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:154612/69‑1 (MQ=255) aTCGAGCACTGCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTaaa < 1:492390/69‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:600721/68‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:640459/68‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:756108/68‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:3232289/68‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:3226409/68‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:958478/68‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:2239827/68‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:1092663/68‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:128007/68‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:1541444/68‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:2227285/68‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:2505487/68‑1 (MQ=255) gCACTTCTTCCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACg < 1:2993713/68‑1 (MQ=255) tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:2836492/1‑65 (MQ=255) tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:3111404/1‑65 (MQ=255) tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:1300758/1‑65 (MQ=255) tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:1332162/1‑65 (MQ=255) tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:1437212/1‑65 (MQ=255) tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:733557/1‑65 (MQ=255) tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:178172/1‑65 (MQ=255) tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:2929457/1‑65 (MQ=255) tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:2535184/1‑65 (MQ=255) tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGat > 1:3374266/1‑65 (MQ=255) tcttcCGTTCGCTTTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGa > 1:31965/1‑64 (MQ=255) tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACCCCTGAAAACCAAAGGTAAAAACGatcatc > 1:609244/1‑69 (MQ=255) tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:566816/1‑69 (MQ=255) tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:3140858/1‑69 (MQ=255) tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:546955/1‑69 (MQ=255) tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:2342740/1‑69 (MQ=255) tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:1666756/1‑69 (MQ=255) tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:3048015/1‑69 (MQ=255) tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:150423/1‑69 (MQ=255) tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:3292324/1‑69 (MQ=255) tcttcCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:1105213/1‑69 (MQ=255) tcttcCGTTCGCTCTCATACACCAAGGGCGAGACGCTACACCTTAAAACCAAAGGTAAAAACGa > 1:1254214/1‑64 (MQ=255) tcttcAGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGatcatc > 1:2684736/1‑69 (MQ=255) | GAGTTTACCTACCAGCGCGTGGGCGATCTCAGCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAAAACGATCATC > minE/1314595‑1314715 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |