| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,315,519 | C→T | 72.3% | A39A (GCC→GCT) | hisA → | N‑(5'‑phospho‑L‑ribosyl‑formimino)‑5‑amino‑1‑ (5'‑phosphoribosyl)‑4‑imidazolecarboxamide isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,315,519 | 0 | C | T | 72.3% | 34.8 / 11.6 | 36 | A39A (GCC→GCT) | hisA | N‑(5'‑phospho‑L‑ribosyl‑formimino)‑5‑amino‑1‑ (5'‑phosphoribosyl)‑4‑imidazolecarboxamide isomerase |
| Reads supporting (aligned to +/- strand): ref base C (8/2); new base T (18/8); total (26/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGCAAACAGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTC > minE/1315464‑1315583 | aCGGCAAACAGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGc > 1:2108553/1‑67 (MQ=255)aCGGCAAACAGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGc > 1:1911531/1‑67 (MQ=255)aCGGCAAACAGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGc > 1:927830/1‑67 (MQ=255)aCGGCAAACAGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGc > 1:1736672/1‑67 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:3404808/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:649802/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:882186/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:2377863/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:2272251/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:2051924/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:1886435/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:1854385/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:946040/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:1674289/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:1542165/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:144146/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:1327858/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:1269837/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:1028532/1‑68 (MQ=255) aacaacGCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAAtgt > 1:2525995/1‑68 (MQ=255) aacaacGCGATTACGGTAACAAACCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:1683965/1‑68 (MQ=255) aacaacCCGATTACGGTAACAACCCGCTGCCGCGCTTACAGGATTACGCTGCGCAAGGTGCCGAAGtgt > 1:356262/1‑68 (MQ=255) cAGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGtgctg > 1:2060813/1‑69 (MQ=255) cAGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGtgctg > 1:1274825/1‑69 (MQ=255) cAGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGtgctg > 1:3318572/1‑69 (MQ=255) aaCGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTg < 1:924048/69‑1 (MQ=255) aaCGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTg < 1:12347/69‑1 (MQ=255) tGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGAt > 1:1747396/1‑47 (MQ=255) aCGCTGCGCAAGGTGCCGAAGTGTTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAGCGTc < 1:3126171/69‑1 (MQ=255) aCGCTGCGCAAGGTGCCGAAGTGTTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAGCGTc < 1:522825/69‑1 (MQ=255) aCGCTGCGCAAGGTGCCGAAGTGTTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAGCGTc < 1:3102762/69‑1 (MQ=255) aCGCTGCGCAAGGTGCCGAAGTGTTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAGCGTc < 1:3046267/69‑1 (MQ=255) aCGCTGCGCAAGGTGCCGAAGTGTTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAGCGTc < 1:2606742/69‑1 (MQ=255) aCGCTGCGCAAGGTGCCGAAGTGTTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAGCGTc < 1:1892801/69‑1 (MQ=255) aCGCTGCGCAAGGTGCCGAAGTGTTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAGCGTc < 1:972617/69‑1 (MQ=255) aCGCTGCGCAAGGTGCCGAAGTGTTGCACCTGGTGGATCTAACCGGGGCAAAAGATCCGGCTAAGCGTc < 1:444218/69‑1 (MQ=255) | ACGGCAAACAGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTC > minE/1315464‑1315583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |