| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 105,158 | G→A | 40.7% | S378S (AGC→AGT) | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 105,158 | 0 | G | A | 40.7% | 31.7 / 52.1 | 76 | S378S (AGC→AGT) | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base G (23/22); new base A (14/17); total (37/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
CAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATT > minE/105092‑105206 | cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:985512/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:2553230/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:2557373/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:2256391/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:2239245/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:2217730/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:300396/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:3133050/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:3429309/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:1670859/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:395133/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:505933/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:663380/1‑69 (MQ=255)cagggcagggAAGCGAGTTACCACGCCTTGTTCTTGTTTGGCACGACGGAATTTCATATGCGCCAGACt > 1:1438798/1‑69 (MQ=255) agcagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAat < 1:389966/69‑1 (MQ=255) agcagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAat < 1:276570/69‑1 (MQ=255) agcagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAat < 1:2867784/69‑1 (MQ=255) agcagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAat < 1:2349422/69‑1 (MQ=255) cagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAatca > 1:1770503/1‑69 (MQ=255) cagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAatca > 1:3239351/1‑69 (MQ=255) cagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAatca > 1:157331/1‑69 (MQ=255) cagGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGACAGGCTAatca > 1:322880/1‑69 (MQ=255) ggAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg < 1:324001/69‑1 (MQ=255) ggAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg < 1:2458481/69‑1 (MQ=255) ggAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg < 1:2405033/69‑1 (MQ=255) ggAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg < 1:2661706/69‑1 (MQ=255) gAAGCGTGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:443248/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:2708161/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:2828677/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:65772/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:642834/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:530296/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:293280/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:3024688/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:3188176/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:1953191/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:1424792/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:1688739/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:1204387/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:1936409/1‑68 (MQ=255) gAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg > 1:2320139/1‑68 (MQ=255) cGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAg < 1:3001362/69‑1 (MQ=255) cGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAg < 1:2502304/69‑1 (MQ=255) cGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAg < 1:3186164/69‑1 (MQ=255) gTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTg < 1:2965201/69‑1 (MQ=255) aCCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGAtt > 1:203628/1‑69 (MQ=255) aCCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGAtt > 1:1057318/1‑69 (MQ=255) aCCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGAtt > 1:1878790/1‑69 (MQ=255) aCCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGAtt > 1:1845618/1‑69 (MQ=255) tGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCa < 1:2391600/69‑1 (MQ=255) tGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCa < 1:57120/69‑1 (MQ=255) tGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCa < 1:3065525/69‑1 (MQ=255) tGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCa < 1:2343717/69‑1 (MQ=255) tGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCa < 1:3285303/69‑1 (MQ=255) tGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATac < 1:657956/68‑1 (MQ=255) gCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCg < 1:88913/42‑1 (MQ=255) gCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATacc < 1:741337/68‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:1286093/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:113931/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:1163476/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:567924/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:1357487/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:1513189/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:418488/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:1529851/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:1671095/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:367958/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:172190/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:3194644/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:3162080/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:2053284/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:2213128/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:2358946/67‑1 (MQ=255) acgACGGAATTTCATATGCGCCAGACTGATCATCGCCCAGTTGATGACCAGTGCAGATACCACCAgcg < 1:2705610/67‑1 (MQ=255) cGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCAtt < 1:63485/69‑1 (MQ=255) cGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCAtt < 1:87605/69‑1 (MQ=255) | CAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATT > minE/105092‑105206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |