| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,315,909 | G→A | 22.4% | L169L (CTG→CTA) | hisA → | N‑(5'‑phospho‑L‑ribosyl‑formimino)‑5‑amino‑1‑ (5'‑phosphoribosyl)‑4‑imidazolecarboxamide isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,315,909 | 0 | G | A | 22.4% | 96.6 / 19.2 | 71 | L169L (CTG→CTA) | hisA | N‑(5'‑phospho‑L‑ribosyl‑formimino)‑5‑amino‑1‑ (5'‑phosphoribosyl)‑4‑imidazolecarboxamide isomerase |
| Reads supporting (aligned to +/- strand): ref base G (35/20); new base A (5/11); total (40/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.28e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01 | |||||||||||
GAGAACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATGAAGAAGTGTG > minE/1315841‑1315974 | gagaACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCtg < 1:50379/69‑1 (MQ=255)gagaACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCtg < 1:501934/69‑1 (MQ=255)gagaACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCtg < 1:2296508/69‑1 (MQ=255)gagaACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCtg < 1:1352893/69‑1 (MQ=255)gagaACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCtg < 1:2695178/69‑1 (MQ=255) acaacTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGAc < 1:1464400/67‑1 (MQ=255) acTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCTACGGCAc > 1:308032/1‑69 (MQ=255) acTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCa > 1:1786724/1‑68 (MQ=255) acTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCAc > 1:1564436/1‑69 (MQ=255) acTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCAc > 1:1085108/1‑69 (MQ=255) acTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCAc > 1:575551/1‑69 (MQ=255) acTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCAc > 1:2378882/1‑69 (MQ=255) acTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCAc > 1:399113/1‑69 (MQ=255) acTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCAc > 1:360008/1‑69 (MQ=255) acTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCAc > 1:728662/1‑69 (MQ=255) acTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCAc > 1:1256995/1‑69 (MQ=255) acTGGTGGAAACCTATATGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCAc > 1:920225/1‑69 (MQ=255) gAACCCTATCTGCCCGTCGGCCTGAAACATGTACTGTGTACCGATATCTCGCGCGAc > 1:2700697/1‑57 (MQ=255) gAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATAtctc > 1:2718577/1‑50 (MQ=255) gAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGc > 1:3270529/1‑68 (MQ=255) gAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTATGTACCGATATCTCGCGCGCCGGCACGCTGGc > 1:1788339/1‑68 (MQ=255) gAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGc > 1:1018727/1‑68 (MQ=255) gAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGc > 1:2547188/1‑68 (MQ=255) gAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGc > 1:482039/1‑68 (MQ=255) gAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGc > 1:701196/1‑68 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTgg > 1:1529042/1‑62 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGc > 1:2166675/1‑67 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGc > 1:3262602/1‑67 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGc > 1:2898482/1‑67 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGc > 1:1192337/1‑67 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGc > 1:3414837/1‑67 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGc > 1:352050/1‑67 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGc > 1:1261887/1‑67 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGc > 1:1312801/1‑67 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGc > 1:2614664/1‑67 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGc > 1:404708/1‑67 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGc > 1:1637122/1‑67 (MQ=255) cTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACAGATAtct > 1:1549884/1‑44 (MQ=255) tATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGTCAGGctct < 1:2321769/69‑1 (MQ=255) tATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGctct < 1:1221250/69‑1 (MQ=255) tATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGctct < 1:530093/69‑1 (MQ=255) tATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGctct < 1:2806994/69‑1 (MQ=255) tATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGctct < 1:282054/69‑1 (MQ=255) tATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGctct < 1:2195704/69‑1 (MQ=255) tATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGctct < 1:786666/69‑1 (MQ=255) aTCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGctct < 1:556360/68‑1 (MQ=255) aTCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGctct < 1:2091877/68‑1 (MQ=255) tGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACg > 1:500571/1‑69 (MQ=255) tGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACg > 1:94194/1‑69 (MQ=255) tGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACg > 1:1905805/1‑69 (MQ=255) tGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACg > 1:2056397/1‑69 (MQ=255) ccGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAg > 1:2664713/1‑57 (MQ=255) ggCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTta < 1:3291083/69‑1 (MQ=255) gCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGCggg < 1:2282687/52‑1 (MQ=255) gCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGCggg < 1:1077941/52‑1 (MQ=255) gCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGCggg < 1:927434/52‑1 (MQ=255) gCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGCggg < 1:1081333/52‑1 (MQ=255) gCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGCggg < 1:1118527/52‑1 (MQ=255) gCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGCggg < 1:67825/52‑1 (MQ=255) gCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGCggg < 1:3111488/52‑1 (MQ=255) gCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGCggg < 1:2300848/52‑1 (MQ=255) gCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGCggg < 1:3427236/52‑1 (MQ=255) gCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGCACGCTGGCggg < 1:2710114/52‑1 (MQ=255) gCCTGAAACATGTGCTATGTACCGATATCTCGCGCGACGGAACGCTGGCggg < 1:2695868/52‑1 (MQ=255) cTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTta > 1:2500532/1‑66 (MQ=255) cTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTta > 1:660868/1‑66 (MQ=255) cTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTta > 1:3016829/1‑66 (MQ=255) tGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATg < 1:487903/69‑1 (MQ=255) tGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATg < 1:2524973/69‑1 (MQ=255) tGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATg < 1:2029192/69‑1 (MQ=255) tGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATg < 1:2599764/69‑1 (MQ=255) tGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATg < 1:616246/69‑1 (MQ=255) tGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATg < 1:126681/69‑1 (MQ=255) tGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATg < 1:3001525/69‑1 (MQ=255) tgtgCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATgaa < 1:1612463/64‑1 (MQ=255) cTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATGAAGAAGtgtg < 1:1866283/68‑1 (MQ=255) | GAGAACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATGAAGAAGTGTG > minE/1315841‑1315974 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |