| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,316,241 | T→C | 23.1% | A40A (GCT→GCC) | hisF → | imidazole glycerol phosphate synthase, catalytic subunit with HisF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,316,241 | 0 | T | C | 23.1% | 147.3 / 37.0 | 112 | A40A (GCT→GCC) | hisF | imidazole glycerol phosphate synthase, catalytic subunit with HisF |
| Reads supporting (aligned to +/- strand): ref base T (61/25); new base C (17/9); total (78/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.40e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
GAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGT > minE/1316175‑1316300 | gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:2327301/68‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:3259459/68‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:1621760/68‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:3220290/68‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:3137102/68‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:343066/68‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:484941/68‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:2988166/68‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:735749/68‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:2495345/68‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCga < 1:2771786/69‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCga < 1:3319325/69‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCga < 1:1081371/69‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCga < 1:1928300/69‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCga < 1:1014874/69‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCga < 1:1580153/69‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCga < 1:1454834/69‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCga < 1:151073/69‑1 (MQ=255)gAAAGGCGTACAGTTTCGCAACCATGAAATCATAGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:3396918/68‑1 (MQ=255) aaaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGTCAAAACGCTACGCTg < 1:463980/67‑1 (MQ=255) aaaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:2798359/67‑1 (MQ=255) aaaGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:2719347/67‑1 (MQ=255) aaaGGCGTACAGTATCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:1873204/67‑1 (MQ=255) ggCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTgaagaa > 1:3161976/1‑69 (MQ=255) ggCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTgaagaa > 1:1046165/1‑69 (MQ=255) ggCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTgaagaa > 1:397026/1‑69 (MQ=255) ggCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTgaaga > 1:1112606/1‑68 (MQ=255) cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCt > 1:335646/1‑69 (MQ=255) cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCt > 1:1254514/1‑69 (MQ=255) cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCt > 1:1280318/1‑69 (MQ=255) cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCt > 1:3423386/1‑69 (MQ=255) cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCt > 1:710778/1‑69 (MQ=255) cAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCt > 1:1636646/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGa > 1:721281/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGa > 1:2866380/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGa > 1:3387736/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGatg > 1:3191027/1‑66 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:3397259/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:2626506/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:2574373/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:926920/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:2365800/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:2560506/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:150320/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:1432017/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:1973648/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:1953138/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:1994539/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:1945068/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:1804803/1‑69 (MQ=255) tCGCAACCATGAAATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGa > 1:2235109/1‑69 (MQ=255) cAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTg < 1:2136395/50‑1 (MQ=255) cATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgt > 1:1887689/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:3187635/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:1621837/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:1451122/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:1851002/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:1439842/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:1435297/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:3226056/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:1956467/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:1989036/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:1384436/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:406950/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:1242615/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtgt > 1:915027/1‑68 (MQ=255) tGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGgtg > 1:1657747/1‑67 (MQ=255) cATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGa < 1:256602/69‑1 (MQ=255) atCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGatat > 1:2747796/1‑62 (MQ=255) atCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGatat > 1:362123/1‑62 (MQ=255) atCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGatat > 1:546969/1‑62 (MQ=255) atCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGatat > 1:1308425/1‑62 (MQ=255) atCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGatat > 1:1616861/1‑62 (MQ=255) tCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCt > 1:1359087/1‑68 (MQ=255) tCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCt > 1:2275041/1‑68 (MQ=255) tCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCt > 1:3231121/1‑68 (MQ=255) tCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCt > 1:2432980/1‑68 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTGCGATATCACCGCTTcc > 1:991504/1‑69 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTcc > 1:1083667/1‑69 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTcc > 1:2182301/1‑69 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTcc > 1:742862/1‑69 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTcc > 1:2578107/1‑69 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTcc > 1:2022199/1‑69 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTcc > 1:2022418/1‑69 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTcc > 1:1338678/1‑69 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTcc > 1:54602/1‑69 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTcc > 1:2081216/1‑69 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTcc > 1:563055/1‑69 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTcc > 1:635759/1‑69 (MQ=255) gTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTcc > 1:2424740/1‑69 (MQ=255) ccGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGc < 1:3276121/69‑1 (MQ=255) ccGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGc < 1:3117532/69‑1 (MQ=255) ccGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGc < 1:258959/69‑1 (MQ=255) acgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCgtgt < 1:1918070/69‑1 (MQ=255) acgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCgtgt < 1:1319812/69‑1 (MQ=255) acgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCgtgt < 1:2821204/69‑1 (MQ=255) acgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCgtgt < 1:2865931/69‑1 (MQ=255) acgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCgtgt < 1:930903/69‑1 (MQ=255) acgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCgtgt < 1:969262/69‑1 (MQ=255) acgctacgctGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCgtgt < 1:2951343/69‑1 (MQ=255) | GAAAGGCGTACAGTTTCGCAACCATGAAATCATTGGCGATATCGTGCCGCTGGCAAAACGCTACGCTGAAGAAGGCGCTGACGAACTGGTGTTCTACGATATCACCGCTTCCAGCGATGGCCGTGT > minE/1316175‑1316300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |