| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,318,439 | T→A | 18.4% | G47G (GGA→GGT) | cld ← | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,318,439 | 0 | T | A | 18.4% | 222.9 / 36.4 | 113 | G47G (GGA→GGT) | cld | regulator of length of O‑antigen component of lipopolysaccharide chains |
| Reads supporting (aligned to +/- strand): ref base T (59/33); new base A (13/8); total (72/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 119 total aligned reads displayed.
TGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTG > minE/1318374‑1318504 | tGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:1095078/1‑68 (MQ=255)tGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:2042219/1‑68 (MQ=255)tGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:637739/1‑68 (MQ=255)tGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:1421625/1‑68 (MQ=255) cACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAAt > 1:2124638/1‑66 (MQ=255) cACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATa > 1:501967/1‑67 (MQ=255) cACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATa > 1:516915/1‑67 (MQ=255) cACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATa > 1:2928556/1‑67 (MQ=255) cACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATa > 1:3284642/1‑67 (MQ=255) cACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATa > 1:2420501/1‑67 (MQ=255) cACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATa > 1:2930475/1‑67 (MQ=255) cACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATa > 1:1425524/1‑67 (MQ=255) cACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATa > 1:2720911/1‑67 (MQ=255) cACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATa > 1:1537715/1‑67 (MQ=255) gggCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGc < 1:2311544/69‑1 (MQ=255) gggCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGc < 1:2021327/69‑1 (MQ=255) gggCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATAGCCAGGGc < 1:216774/69‑1 (MQ=255) gggCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATAGCCAGGGc < 1:1667328/69‑1 (MQ=255) gggCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATAGCCAGGGc < 1:750310/69‑1 (MQ=255) gggCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATAGCCAGGGc < 1:2921220/69‑1 (MQ=255) ggCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATAGCCAGGGc < 1:3171003/68‑1 (MQ=255) cTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCaa > 1:1143262/1‑68 (MQ=255) cTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAAt > 1:3319307/1‑69 (MQ=255) gATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCcac > 1:706369/1‑69 (MQ=255) gATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCcac > 1:2196885/1‑69 (MQ=255) gATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCcac > 1:136167/1‑69 (MQ=255) aTTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:1371197/1‑44 (MQ=255) aTTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:1742315/1‑44 (MQ=255) aTTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:3163530/1‑44 (MQ=255) aTTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:3136980/1‑44 (MQ=255) ttGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATAACCAATAGCCAGGGc < 1:3242806/55‑1 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:1423162/1‑35 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:2287737/1‑35 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:1622616/1‑35 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:1483251/1‑35 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATcc > 1:452288/1‑35 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAat < 1:1882637/68‑1 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAat < 1:2507851/68‑1 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAat < 1:2790729/68‑1 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAat < 1:3156994/68‑1 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAat < 1:1353378/68‑1 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAat < 1:2027178/68‑1 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAat < 1:141058/68‑1 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAat < 1:1323383/68‑1 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAat < 1:1810686/68‑1 (MQ=255) gACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAat < 1:1687239/68‑1 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGc < 1:3235433/35‑1 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGa > 1:2361361/1‑59 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGa > 1:2795290/1‑59 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGa > 1:1600974/1‑59 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGa > 1:3179774/1‑59 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGa > 1:1634001/1‑59 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGa > 1:372978/1‑59 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGa < 1:3251524/59‑1 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGa < 1:1709039/59‑1 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGa < 1:1184234/59‑1 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGa > 1:862/1‑59 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGa > 1:332288/1‑59 (MQ=255) cATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGa > 1:3008909/1‑59 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGAtt > 1:1874532/1‑67 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:815073/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:667002/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:891189/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:79931/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:3250242/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:624083/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:1132932/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:434206/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:3376343/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:2835672/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:1159970/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:1439938/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:1489190/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:1639720/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:1811447/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:2215033/1‑69 (MQ=255) tttCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGt > 1:288889/1‑69 (MQ=255) tttCACCTTCGCTACTGCCAAATATCCAATAGCCAGGGCTATAGCCACAATGACGGAAATGAAGATTGt > 1:1161249/1‑69 (MQ=255) tctcCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCa > 1:795531/1‑69 (MQ=255) tctcCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCa > 1:2758137/1‑69 (MQ=255) tctcCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCa > 1:3417228/1‑69 (MQ=255) tctcCTTCGCTAATGCCAAATATCCAATAGCCAGGGCAATAGc > 1:1879474/1‑43 (MQ=255) tCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgc < 1:2500146/69‑1 (MQ=255) cTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgcca < 1:1716973/68‑1 (MQ=255) cTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgcca < 1:2336848/68‑1 (MQ=255) cTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgcca < 1:2328247/68‑1 (MQ=255) cTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgcca < 1:1305057/68‑1 (MQ=255) cTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgcca < 1:3314890/68‑1 (MQ=255) cTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgcca < 1:1549091/68‑1 (MQ=255) cTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgcca < 1:261363/68‑1 (MQ=255) cTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgcca < 1:2105449/68‑1 (MQ=255) cTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgcca < 1:2555767/68‑1 (MQ=255) cTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgcca < 1:1972856/68‑1 (MQ=255) cTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgcca < 1:2101489/68‑1 (MQ=255) gCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTTTCATCTTgccacgccac < 1:1996949/69‑1 (MQ=255) gCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgccacgccac < 1:2484937/69‑1 (MQ=255) gCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTgccacgccac < 1:2132644/69‑1 (MQ=255) atatCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTg < 1:1758329/69‑1 (MQ=255) tCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTg > 1:2814922/1‑66 (MQ=255) tCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTg > 1:349761/1‑66 (MQ=255) | TGCCCCACGTCGGGCTGAGTGATAATTGCTGTTGACGTCCATTTCTCCTTCGCTACTGCCAAATATCCAATAGCCAGGGCAATAGCCACAATGACGGAAATGATGATTGTCATCTTGCCACGCCACAACTG > minE/1318374‑1318504 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |