| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,319,007 | C→T | 44.3% | K295K (AAG→AAA) | ugd ← | UDP‑glucose 6‑dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,319,007 | 0 | C | T | 44.3% | 19.4 / 53.0 | 72 | K295K (AAG→AAA) | ugd | UDP‑glucose 6‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (38/2); new base T (17/15); total (55/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGAAGTTATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAGCATCGACAATTG > minE/1318951‑1319068 | cGGAAGTTTTCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACaaa > 1:1809874/1‑69 (MQ=255)cGGAAGTTATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACaaa > 1:2532542/1‑69 (MQ=255) gAAGTTATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAAt > 1:1204903/1‑69 (MQ=255) gAAGTTATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAAt > 1:1253334/1‑69 (MQ=255) gAAGTTATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAAt > 1:1389569/1‑69 (MQ=255) gAAGTTATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAAt > 1:2057176/1‑69 (MQ=255) ttATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATGGc > 1:2277379/1‑68 (MQ=255) ttATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATGGCa > 1:964035/1‑69 (MQ=255) ttATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATGGCa > 1:3219266/1‑69 (MQ=255) tATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAAt > 1:913983/1‑64 (MQ=255) tATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATgg > 1:2241952/1‑66 (MQ=255) tATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATgg > 1:64685/1‑66 (MQ=255) tATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATgg > 1:3435642/1‑66 (MQ=255) tATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATgg > 1:3223094/1‑66 (MQ=255) tATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATgg > 1:1267224/1‑66 (MQ=255) tATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATgg > 1:1198224/1‑66 (MQ=255) tATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATgg > 1:3146801/1‑66 (MQ=255) tATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATgg > 1:1034172/1‑66 (MQ=255) tGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGc < 1:526115/69‑1 (MQ=255) tGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGc > 1:3167990/1‑69 (MQ=255) tGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGc > 1:1797911/1‑69 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAtgcg < 1:1770985/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:934476/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:2850058/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:3049459/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:2581781/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:1002/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:1059489/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:24362/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:1066018/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:1257695/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:1352553/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:523333/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:1575434/68‑1 (MQ=255) cAGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:820094/68‑1 (MQ=255) aGACGATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTAcgcg < 1:1516198/67‑1 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAAt > 1:917819/1‑49 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:581864/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:3287359/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:954395/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:3317254/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:417273/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:3134045/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:3119448/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:987109/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:2960064/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:1187204/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:2666912/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:2283899/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:2214077/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:182781/1‑50 (MQ=255) gATAAATACCCACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:174552/1‑50 (MQ=255) gATAAATACCAACCACTTGCGGTTTGCGTGACAAAATGGCATCGGCAATa > 1:1827491/1‑50 (MQ=255) tAAATACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGtt > 1:2557472/1‑68 (MQ=255) tAAATACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGtt > 1:1319999/1‑68 (MQ=255) tAAATACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGtt > 1:1577153/1‑68 (MQ=255) aaaTACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTa > 1:1217933/1‑68 (MQ=255) aaaTACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAg > 1:2814756/1‑69 (MQ=255) aaaTACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAg > 1:1349754/1‑69 (MQ=255) aaaTACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAg > 1:1436871/1‑69 (MQ=255) aaaTACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAg > 1:556437/1‑69 (MQ=255) aaaTACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAg > 1:2029337/1‑69 (MQ=255) aaaTACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAg > 1:2237582/1‑69 (MQ=255) aaaTACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGAGt > 1:3103384/1‑61 (MQ=255) aaaTAACCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTa > 1:1037163/1‑68 (MQ=255) caccacTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAGCATCGAc < 1:1890772/69‑1 (MQ=255) cacTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAGCATCGACAAt > 1:1686322/1‑69 (MQ=255) cTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAGCATCGACAAtt > 1:794956/1‑68 (MQ=255) cTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAGCATCGACAATTg > 1:1697390/1‑69 (MQ=255) cTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAGCATCGACAATTg > 1:1892051/1‑69 (MQ=255) cTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAGCATCGACAATTg > 1:920398/1‑69 (MQ=255) cTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAGCATCGACAATTg > 1:2547421/1‑69 (MQ=255) | CGGAAGTTATCTGAACCGCTCTTCATAATCAGACGATAAATACCCACCACTTGCGGCTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAGCATCGACAATTG > minE/1318951‑1319068 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |