| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,320,260 | G→A | 51.6% | D429D (GAC→GAT) | gnd ← | gluconate‑6‑phosphate dehydrogenase, decarboxylating |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,320,260 | 0 | G | A | 51.6% | ‑1.9 / 85.0 | 93 | D429D (GAC→GAT) | gnd | gluconate‑6‑phosphate dehydrogenase, decarboxylating |
| Reads supporting (aligned to +/- strand): ref base G (11/34); new base A (27/21); total (38/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.92e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAAC > minE/1320197‑1320325 | cgcACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGt > 1:2972426/1‑68 (MQ=255)cgcACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGt > 1:1888759/1‑68 (MQ=255)cgcACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGt > 1:2094226/1‑68 (MQ=255)cgcACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGt > 1:2286165/1‑68 (MQ=255)cgcACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGt > 1:2300729/1‑68 (MQ=255)cgcACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGt > 1:163925/1‑68 (MQ=255)cgcACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGt > 1:2656958/1‑68 (MQ=255)cgcACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGt > 1:2686574/1‑68 (MQ=255)cgcACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGt > 1:1325899/1‑68 (MQ=255)cgcACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGt > 1:1103941/1‑68 (MQ=255) cACGCTGTGCCTGGATCATGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:472564/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:367967/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:3162677/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:1075354/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:289854/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:278231/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:586664/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:689486/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:818907/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:839867/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:2103559/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:2067971/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:905348/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:1628689/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:1110614/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:1147302/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:1810862/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:1304321/69‑3 (MQ=255) cACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:1321262/69‑3 (MQ=255) aCGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:1356613/68‑3 (MQ=255) aCGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGcag < 1:250438/68‑3 (MQ=255) tgtgCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGgag < 1:2073029/69‑1 (MQ=255) tCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGTTCGGa < 1:1428196/68‑1 (MQ=255) tCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGa < 1:616646/68‑1 (MQ=255) tCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGa < 1:2968685/68‑1 (MQ=255) tCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGa < 1:2919878/68‑1 (MQ=255) tCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGa < 1:24010/68‑1 (MQ=255) gTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:2978700/69‑1 (MQ=255) gTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:1698225/69‑1 (MQ=255) gTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:2401741/69‑1 (MQ=255) gTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:1619640/69‑1 (MQ=255) gTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:744317/69‑1 (MQ=255) gTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:994640/69‑1 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCATCCGCAGCGGCGAAGGTCGGAAc > 1:1393658/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCACCCGCAGCGGCGAAGGTCGGAAc > 1:2496537/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:2028392/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:3212671/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:3243763/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:357020/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:2082954/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:376779/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:460937/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:131263/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:478607/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:3302517/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:1217372/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:2010223/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:75876/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:835634/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:1954267/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:969489/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:1940766/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:2690538/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:2866260/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:2908016/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:2798566/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:2142981/1‑59 (MQ=255) ggcagAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:2987697/1‑59 (MQ=255) ggcagAACAGCGGCACGGTACCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:623894/1‑59 (MQ=255) ggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCg < 1:874966/69‑1 (MQ=255) ggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCg < 1:3431120/69‑1 (MQ=255) ggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCg < 1:1844395/69‑1 (MQ=255) ggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCg < 1:2471536/69‑1 (MQ=255) ggcagAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCg < 1:1836601/69‑1 (MQ=255) ggcacAACAGCGGCACGGTAGCTATCGTAATAGGCAACCGCAGCGGCGAAGGTCGGAAc > 1:3300472/1‑59 (MQ=255) aaCAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTg < 1:2655768/69‑1 (MQ=255) aaCAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTg < 1:1594726/69‑1 (MQ=255) aaCAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTg < 1:3227149/69‑1 (MQ=255) aaCAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTg < 1:1042067/69‑1 (MQ=255) cagcagCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:275536/55‑1 (MQ=255) cagcagCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:716386/55‑1 (MQ=255) cagcagCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:256488/55‑1 (MQ=255) cagcagCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:2367945/55‑1 (MQ=255) cagcagCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:425691/55‑1 (MQ=255) cagcagCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:170788/55‑1 (MQ=255) cagcagCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCgg < 1:3332856/55‑1 (MQ=255) gTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGc > 1:3366656/1‑69 (MQ=255) gCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAAc < 1:2798337/69‑1 (MQ=255) gCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAAc < 1:2009430/69‑1 (MQ=255) gCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAAc < 1:3214456/69‑1 (MQ=255) gCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAAc < 1:314369/69‑1 (MQ=255) gCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAAc < 1:1782148/69‑1 (MQ=255) tGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAAc < 1:2888531/67‑1 (MQ=255) | CGCACCAAAATAGTCACGCTGTGCCTGGATCAGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAAC > minE/1320197‑1320325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |