| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,322,814 | A→G | 39.4% | I40I (ATT→ATC) | galF ← | predicted subunit with GalU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,322,814 | 0 | A | G | 39.4% | 52.4 / 79.5 | 114 | I40I (ATT→ATC) | galF | predicted subunit with GalU |
| Reads supporting (aligned to +/- strand): ref base A (17/52); new base G (31/14); total (48/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.35e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
GCGTTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGT > minE/1322749‑1322873 | gcgTTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATg < 1:3266192/69‑1 (MQ=255)gcgTTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATg < 1:896299/69‑1 (MQ=255)gcgTTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATg < 1:332532/69‑1 (MQ=255)gcgTTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATg < 1:1354303/69‑1 (MQ=255)gcgTTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATg < 1:432248/69‑1 (MQ=255)gcgTTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATg < 1:369281/69‑1 (MQ=255)gcgTTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTAGTCAAC‑AATg < 1:1093737/69‑1 (MQ=255) gaagcgTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑GATGTACTGAATc > 1:586643/4‑69 (MQ=255) gaagcgTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑GATGTACTGAATc > 1:1505847/4‑69 (MQ=255) gaagcgTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑GATGTACTGAATc > 1:1811701/4‑69 (MQ=255) gaagcgTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑GATGTACTGAATc > 1:1829900/4‑69 (MQ=255) gaagcgTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑GATGTACTGAATc > 1:1835454/4‑69 (MQ=255) gaagcgTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑GATGTACTGAATc > 1:769044/4‑69 (MQ=255) gaagcgTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑GATGTACTGAATc > 1:1957152/4‑69 (MQ=255) gaagcgTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑GATGTACTGAATc > 1:2132600/4‑69 (MQ=255) gaagcgTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑GATGTACTGAATc > 1:886721/4‑69 (MQ=255) gaagcgTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑GATGTACTGAATc > 1:2470214/4‑69 (MQ=255) gaagcgTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑GATGTACTGAATc > 1:2830712/4‑69 (MQ=255) gaagcgAGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑GATGTACTGAATc > 1:435584/4‑69 (MQ=255) aCGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAAt < 1:3335036/67‑1 (MQ=255) aCGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAAt < 1:145675/67‑1 (MQ=255) gaggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAAATGTACTGAATc > 1:3001551/1‑53 (MQ=255) gaggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATc > 1:600160/1‑52 (MQ=255) gaggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATc < 1:53094/52‑1 (MQ=255) gaggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATc > 1:3051187/1‑52 (MQ=255) gaggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATc > 1:1505599/1‑52 (MQ=255) gaggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATc > 1:2512067/1‑52 (MQ=255) gaggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATc > 1:2299732/1‑52 (MQ=255) gaggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATc < 1:2259220/52‑1 (MQ=255) gaggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATc > 1:1217571/1‑52 (MQ=255) gaggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATc > 1:1235418/1‑52 (MQ=255) gaggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATc > 1:1925587/1‑52 (MQ=255) gaggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTCAATc > 1:2822262/1‑52 (MQ=255) gaggaTTTCTTTGATCCCTGAAGCCACAATCTCGTCAAC‑AATGTACTGatt > 1:11382/1‑49 (MQ=255) aggaTTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATc < 1:1506860/51‑1 (MQ=255) tttCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGt > 1:3018617/1‑68 (MQ=255) tttCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGt > 1:3151088/1‑68 (MQ=255) ttCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATg > 1:659967/1‑37 (MQ=255) tCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc < 1:281044/69‑1 (MQ=255) tCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc < 1:2717993/69‑1 (MQ=255) tCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc < 1:1369868/69‑1 (MQ=255) tCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc < 1:2357085/69‑1 (MQ=255) tCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCCACGATTGGTAGc < 1:500841/69‑1 (MQ=255) tCTTTGATCCCTGCAGCCACAATCTCATCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc < 1:955442/69‑1 (MQ=255) aTCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCAtct < 1:932872/67‑1 (MQ=255) aTCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCAtct < 1:1043551/67‑1 (MQ=255) aTCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCAtct < 1:1906946/67‑1 (MQ=255) aTCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATCGGCTTGTCGACGATTGGTAGCAtct < 1:334774/67‑1 (MQ=255) aTCCCTGCAGCCACAATCTCATCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCAtct < 1:128591/67‑1 (MQ=255) cacaATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGc < 1:3142137/69‑1 (MQ=255) cacaATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGc < 1:1522053/69‑1 (MQ=255) cacaATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGc < 1:623961/69‑1 (MQ=255) cacaATCTCGTCAAC‑AATGTACTGAATCATTGGATTGTCGACGATTGGTAGCATCTCTTTGGGTATCGc < 1:8075/69‑1 (MQ=255) aaTCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:3048215/69‑1 (MQ=255) aaTCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:3052536/69‑1 (MQ=255) aaTCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:855889/69‑1 (MQ=255) aaTCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:417349/69‑1 (MQ=255) aaTCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:2637155/69‑1 (MQ=255) aaTCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:2858214/69‑1 (MQ=255) aaTCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:1268559/69‑1 (MQ=255) aaTCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:162715/69‑1 (MQ=255) aaTCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:181540/69‑1 (MQ=255) aaTCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:1234184/69‑1 (MQ=255) aaTCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:118408/69‑1 (MQ=255) aaTCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:2132728/69‑1 (MQ=255) aTCTCGTCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:796206/69‑1 (MQ=255) aTCTCGTCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:1265464/69‑1 (MQ=255) aTCTCGTCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:38032/69‑1 (MQ=255) aTCTCGTCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:2178689/69‑1 (MQ=255) aTCTCGTCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:2396689/69‑1 (MQ=255) aTCTCGTCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:2771730/69‑1 (MQ=255) aTCTCGTCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:247057/69‑1 (MQ=255) aTCTCGTCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:2416301/69‑1 (MQ=255) aTCTCGTCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:3194939/69‑1 (MQ=255) tcGTCAAC‑AATGTACTTAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:1305189/56‑1 (MQ=255) tcGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTTGTAGCATCTCTTTggg < 1:3276158/56‑1 (MQ=255) tcGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:1456931/56‑1 (MQ=255) tcGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:2340839/56‑1 (MQ=255) tcGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:423569/56‑1 (MQ=255) tcGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:18846/56‑1 (MQ=255) tcGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:100249/56‑1 (MQ=255) tcGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:1683604/56‑1 (MQ=255) tcGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:3032487/56‑1 (MQ=255) tcGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGt > 1:3341716/1‑68 (MQ=255) tcGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGAATGGTAGCATCTCTTTggg < 1:1759437/56‑1 (MQ=255) tCAAC‑GATGTACTGAATCATTGGGTTGTCGACGATCgg > 1:3250244/1‑38 (MQ=25) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:841078/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:1079180/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:887270/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:108491/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:2970916/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:103793/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:739077/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:1634350/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:1901731/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:1905889/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:2216766/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:3231518/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:3181401/1‑38 (MQ=38) tCAAC‑GATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:2854158/1‑38 (MQ=38) | GCGTTCTTGGACGCGTGAGTTACCAGGAGGATTTCTTTGATCCCTGCAGCCACAATCTCGTCAAC‑AATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGT > minE/1322749‑1322873 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |