Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 1,322,844 | T→C | 32.6% | P30P (CCA→CCG) | galF ← | predicted subunit with GalU |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 1,322,844 | 0 | T | C | 32.6% | 79.7 / 54.1 | 101 | P30P (CCA→CCG) | galF | predicted subunit with GalU |
Reads supporting (aligned to +/- strand): ref base T (7/61); new base C (19/14); total (26/75) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 9.13e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
Only 100 of 107 total aligned reads displayed.
TTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAT > minE/1322780‑1322912 | tttCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGt > 1:3018617/1‑68 (MQ=255) tttCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGt > 1:3151088/1‑68 (MQ=255) tCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc < 1:281044/69‑1 (MQ=255) tCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc < 1:2717993/69‑1 (MQ=255) tCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc < 1:2357085/69‑1 (MQ=255) tCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc < 1:1517419/69‑1 (MQ=255) tCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCCACGATTGGTAGc < 1:500841/69‑1 (MQ=255) tCTTTGATCCCTGCAGCCACAATCTCATCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc < 1:955442/69‑1 (MQ=255) cTTTGATCCCTGCATCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGc < 1:2537936/68‑1 (MQ=255) aTCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCAtct < 1:1906946/67‑1 (MQ=255) aTCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCAtct < 1:932872/67‑1 (MQ=255) aTCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCAtct < 1:1043551/67‑1 (MQ=255) aTCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATCGGCTTGTCGACGATTGGTAGCAtct < 1:334774/67‑1 (MQ=255) aTCCCTGCAGCCACAATCTCATCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCAtct < 1:128591/67‑1 (MQ=255) cacaATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGc < 1:1522053/69‑1 (MQ=255) cacaATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGc < 1:623961/69‑1 (MQ=255) cacaATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGc < 1:3142137/69‑1 (MQ=255) cacaATCTCGTCAACAATGTACTGAATCATTGGATTGTCGACGATTGGTAGCATCTCTTTGGGTATCGc < 1:8075/69‑1 (MQ=255) aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:2858214/69‑1 (MQ=255) aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:2132728/69‑1 (MQ=255) aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:855889/69‑1 (MQ=255) aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:417349/69‑1 (MQ=255) aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:118408/69‑1 (MQ=255) aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:2637155/69‑1 (MQ=255) aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:181540/69‑1 (MQ=255) aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:1268559/69‑1 (MQ=255) aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:162715/69‑1 (MQ=255) aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:3052536/69‑1 (MQ=255) aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:1234184/69‑1 (MQ=255) aaTCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCtt < 1:3048215/69‑1 (MQ=255) aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:2771730/69‑1 (MQ=255) aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:247057/69‑1 (MQ=255) aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:3194939/69‑1 (MQ=255) aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:2396689/69‑1 (MQ=255) aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:2909348/69‑1 (MQ=255) aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:2178689/69‑1 (MQ=255) aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:1265464/69‑1 (MQ=255) aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:38032/69‑1 (MQ=255) aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:810895/69‑1 (MQ=255) aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:796206/69‑1 (MQ=255) aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGGAATCGCCTTa < 1:1825474/69‑1 (MQ=255) aTCTCGTCAACGATGTACTGAATCATTGGCTTGTCGACGATCGGTAGCATCTCTTTGGCAATCGCCTTa < 1:3096873/69‑1 (MQ=255) tcGTCAACAATGTACTTAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:1305189/56‑1 (MQ=255) tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTTGTAGCATCTCTTTggg < 1:3276158/56‑1 (MQ=255) tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:2340839/56‑1 (MQ=255) tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:100249/56‑1 (MQ=255) tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:3032487/56‑1 (MQ=255) tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:423569/56‑1 (MQ=255) tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:18846/56‑1 (MQ=255) tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:1456931/56‑1 (MQ=255) tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTggg < 1:1683604/56‑1 (MQ=255) tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGt > 1:3341716/1‑68 (MQ=255) tcGTCAACAATGTACTGAATCATTGGCTTGTCGACGAATGGTAGCATCTCTTTggg < 1:1759437/56‑1 (MQ=255) tCAACGATGTACTGAATCATTGGGTTGTCGACGATCgg > 1:3250244/1‑38 (MQ=25) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:739077/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:841078/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:887270/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:3181401/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:103793/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:404728/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:1905889/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:2320023/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:2216766/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:1939888/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:1634350/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:1207277/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:108491/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:2854158/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:1079180/1‑38 (MQ=38) tCAACGATGTACTGAATCATTGGCTTGTCGACGATCgg > 1:2970916/1‑38 (MQ=38) tGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa < 1:2646045/69‑1 (MQ=255) tGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa < 1:3172024/69‑1 (MQ=255) tGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa < 1:2460047/69‑1 (MQ=255) tGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa < 1:3246647/69‑1 (MQ=255) tGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa < 1:358037/69‑1 (MQ=255) tGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa < 1:2408776/69‑1 (MQ=255) gTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa < 1:1962361/68‑1 (MQ=255) gTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa < 1:2610437/68‑1 (MQ=255) cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat < 1:3299558/68‑1 (MQ=255) cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat < 1:2977602/68‑1 (MQ=255) cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat < 1:2443642/68‑1 (MQ=255) cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat < 1:2819806/68‑1 (MQ=255) cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat < 1:1210240/68‑1 (MQ=255) cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat < 1:130591/68‑1 (MQ=255) cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat < 1:1881058/68‑1 (MQ=255) cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat < 1:1830757/68‑1 (MQ=255) cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat < 1:742671/68‑1 (MQ=255) cTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATg > 1:2823753/1‑69 (MQ=255) tCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATc > 1:971315/1‑68 (MQ=255) tCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATc > 1:19192/1‑68 (MQ=255) tCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATc > 1:324988/1‑68 (MQ=255) aTTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa < 1:1099695/58‑1 (MQ=255) gacgaTTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACatat < 1:908149/50‑1 (MQ=255) cgaTTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACa < 1:688100/45‑1 (MQ=255) tGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAt < 1:1558718/69‑1 (MQ=255) tGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAt < 1:1493053/69‑1 (MQ=255) tGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAt < 1:3154291/69‑1 (MQ=255) tGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAt < 1:406049/69‑1 (MQ=255) tGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAt < 1:2958647/69‑1 (MQ=255) tGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAt < 1:2358458/69‑1 (MQ=255) | TTTCTTTGATCCCTGCAGCCACAATCTCGTCAACAATGTACTGAATCATTGGCTTGTCGACGATTGGTAGCATCTCTTTGGGTATCGCCTTAGTGGCAGGCAACATATGCATCCCGAGACCCGCTACAGGAAT > minE/1322780‑1322912 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |