Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 105,440 C→T 40.7% V284V (GTG→GTA aroP ← aromatic amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE105,4400CT40.7% 34.8 / 55.6 81V284V (GTG→GTAaroParomatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base C (28/20);  new base T (14/19);  total (42/39)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

GCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCG  >  minE/105374‑105502
                                                                  |                                                              
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:3025389/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:929683/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:632637/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:898352/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:2556272/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:2694198/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:286494/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:2921027/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:3014553/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:1404736/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:3036499/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:3065982/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:131069/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:3100723/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:3107151/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:388002/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:961627/1‑68 (MQ=255)
gCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCca                                                               >  1:873526/1‑68 (MQ=255)
      taCGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGt                                                        >  1:1743371/1‑69 (MQ=255)
      taCGCAGCTGTTGAACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGt                                                        >  1:2466896/1‑69 (MQ=255)
             cTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCt                                                 >  1:3073064/1‑69 (MQ=255)
             cTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCt                                                 <  1:647878/69‑1 (MQ=255)
             cTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCt                                                 <  1:2879870/69‑1 (MQ=255)
             cTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCt                                                 <  1:277597/69‑1 (MQ=255)
             cTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCt                                                 >  1:437566/1‑69 (MQ=255)
             cTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCt                                                 <  1:68098/69‑1 (MQ=255)
             cTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCt                                                 <  1:246815/69‑1 (MQ=255)
             cTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCt                                                 >  1:400112/1‑69 (MQ=255)
                  gtACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTc                                            >  1:193250/1‑69 (MQ=255)
                  gtACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTc                                            >  1:622499/1‑69 (MQ=255)
                     cacGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGt                                          >  1:701018/1‑68 (MQ=255)
                     cacGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGt                                          >  1:129150/1‑68 (MQ=255)
                     cacGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGt                                          >  1:385756/1‑68 (MQ=255)
                         gagaGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAg                                     <  1:475606/69‑1 (MQ=255)
                         gagaGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAg                                     <  1:247214/69‑1 (MQ=255)
                         gagaGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAg                                     <  1:951728/69‑1 (MQ=255)
                         gagaGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAg                                     <  1:2670169/69‑1 (MQ=255)
                         gagaGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAg                                     <  1:753271/69‑1 (MQ=255)
                                      tcagtACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACg                        <  1:264130/69‑1 (MQ=255)
                                      tcagtACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACg                        <  1:1393758/69‑1 (MQ=255)
                                      tcagtACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACg                        <  1:1579403/69‑1 (MQ=255)
                                      tcagtACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACg                        <  1:943426/69‑1 (MQ=255)
                                        agtACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACgg                       >  1:51502/1‑68 (MQ=255)
                                        agtACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACgg                       >  1:2435676/1‑68 (MQ=255)
                                        agtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:2723403/69‑1 (MQ=255)
                                        agtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:955973/69‑1 (MQ=255)
                                        agtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:3027296/69‑1 (MQ=255)
                                        agtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:856920/69‑1 (MQ=255)
                                        agtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:727440/69‑1 (MQ=255)
                                        agtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:3308303/69‑1 (MQ=255)
                                        agtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:690573/69‑1 (MQ=255)
                                        agtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:2806274/69‑1 (MQ=255)
                                        agtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:509065/69‑1 (MQ=255)
                                        agtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:2678329/69‑1 (MQ=255)
                                        agtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:1903032/69‑1 (MQ=255)
                                         gtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:144113/68‑1 (MQ=255)
                                         gtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:1859849/68‑1 (MQ=255)
                                         gtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:2657395/68‑1 (MQ=255)
                                         gtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:2446022/68‑1 (MQ=255)
                                         gtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:947865/68‑1 (MQ=255)
                                         gtACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:634955/68‑1 (MQ=255)
                                          tACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:1021132/67‑1 (MQ=255)
                                          tACCACGATATTCAGCGCATTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGa                      <  1:3213252/67‑1 (MQ=255)
                                            ccACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTgg                  >  1:1318711/1‑69 (MQ=255)
                                            ccACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTgg                  >  1:3171769/1‑69 (MQ=255)
                                            ccACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTgg                  <  1:3036465/69‑1 (MQ=255)
                                            ccACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTgg                  <  1:704871/69‑1 (MQ=255)
                                            ccACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTgg                  >  1:2262138/1‑69 (MQ=255)
                                            ccACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTgg                  >  1:2412467/1‑69 (MQ=255)
                                              aCGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGt                 <  1:901042/68‑1 (MQ=255)
                                              aCGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGt                 <  1:609885/68‑1 (MQ=255)
                                               cGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGt                 <  1:1938954/67‑1 (MQ=255)
                                                   gTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAg                                     <  1:2224287/43‑1 (MQ=255)
                                                      cAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGa                                       >  1:867566/1‑38 (MQ=255)
                                                      cAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACg                        >  1:2678768/1‑53 (MQ=255)
                                                      cAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACg                        >  1:2803007/1‑53 (MQ=255)
                                                      cAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACg                        >  1:2818172/1‑53 (MQ=255)
                                                      cAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACg                        >  1:154010/1‑53 (MQ=255)
                                                      cAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACg                        >  1:1503405/1‑53 (MQ=255)
                                                      cAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACg                        >  1:1205082/1‑53 (MQ=255)
                                                           cATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGa                                    >  1:2162285/1‑36 (MQ=255)
                                                           cATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGa                                    >  1:1694888/1‑36 (MQ=255)
                                                           cATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGa                                    >  1:1541600/1‑36 (MQ=255)
                                                           cATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGa                                    >  1:2861656/1‑36 (MQ=255)
                                                           cATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGa                                    >  1:3408494/1‑36 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGAt                                   >  1:859771/1‑36 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTACcgcc  >  1:343780/1‑68 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAcgcg  >  1:987883/1‑69 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAcgcg  >  1:62213/1‑69 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAcgcg  >  1:2073784/1‑69 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAcgcg  >  1:1852318/1‑69 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAcgcg  >  1:1786423/1‑69 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAcgcg  >  1:1303734/1‑69 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAcgcg  >  1:1134778/1‑69 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAcgcg  >  1:1034910/1‑69 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAcgc   >  1:861319/1‑68 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAcgc   >  1:877011/1‑68 (MQ=255)
                                                            aTTCGCTACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAAcg    >  1:3306112/1‑67 (MQ=255)
                                                            aTTCGCTACAAAGGTATCCCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATcggcg          >  1:1625505/1‑61 (MQ=255)
                                                                  |                                                              
GCAATATACGCAGCTGTTGTACACGGAGAGCGCCGCAGTCAGTACCACGATGTTCAGCGCATTCGCCACAAAGGTATCGCCTAACTCGTGGAAGATCAGCACAAACGGACTGGTATCGGCGGTAACGCG  >  minE/105374‑105502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: