| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,325,809 | C→T | 23.8% | S401N (AGT→AAT) ‡ | wcaK ← | predicted pyruvyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,325,809 | 0 | C | T | 23.8% | 91.4 / 20.9 | 71 | S401N (AGT→AAT) ‡ | wcaK | predicted pyruvyl transferase |
| Reads supporting (aligned to +/- strand): ref base C (28/26); new base T (9/8); total (37/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
TGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCGCTTGCA > minE/1325773‑1325866 | tGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCa > 1:3065589/1‑48 (MQ=255)tGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCa > 1:2757351/1‑48 (MQ=255)tGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCa > 1:2455628/1‑48 (MQ=255)tGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCa > 1:2038858/1‑48 (MQ=255)tGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCa > 1:1681624/1‑48 (MQ=255)tGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCa > 1:1809017/1‑48 (MQ=255)tGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCa > 1:1804963/1‑48 (MQ=255) cATTCCCGTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTaa > 1:1029790/1‑69 (MQ=255) cATTCCCGTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTaa > 1:2850444/1‑69 (MQ=255) cATTCCCGTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTaa > 1:2812535/1‑69 (MQ=255) cATTCCCGTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTaa > 1:3279818/1‑69 (MQ=255) cATTCCCGTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTaa > 1:3408956/1‑69 (MQ=255) cATTCCCGTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTaa > 1:219926/1‑69 (MQ=255) cATTCCCGTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTaa > 1:1876401/1‑69 (MQ=255) cATTCCCGTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTaa > 1:706214/1‑69 (MQ=255) cATTCCCGTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTaa > 1:1127694/1‑69 (MQ=255) ttCCTGTCTGACGCTCACTACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCt < 1:2404167/65‑1 (MQ=255) ccTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTa < 1:1925704/69‑1 (MQ=255) ccTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTa > 1:3178922/1‑69 (MQ=255) ccTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTa < 1:859209/69‑1 (MQ=255) ccTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTa > 1:3304678/1‑69 (MQ=255) ccTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGACGGAAGCTGGCCTAAGGTa > 1:1520830/1‑69 (MQ=255) gTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTAAGGTAccc < 1:182495/69‑1 (MQ=255) gTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTAAGGTATcc < 1:2260783/69‑1 (MQ=255) gTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTAAGGTATcc < 1:2890764/69‑1 (MQ=255) gTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTAAGGTATcc < 1:2504157/69‑1 (MQ=255) gTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTAAGGTATcc < 1:3016424/69‑1 (MQ=255) gTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTAAGGTATcc < 1:571624/69‑1 (MQ=255) gTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTAAGGTATcc < 1:58148/69‑1 (MQ=255) gTCTGACGTTCGCGACTGACAGCTTCGTTAAGTCGTGCGTTCAGCGCCGGAAGCTGGCCTAAGGTATcc < 1:929719/69‑1 (MQ=255) cTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTTGCCTAAGGTATCCGc > 1:1351866/1‑69 (MQ=255) cTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc > 1:2383085/1‑69 (MQ=255) cTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc > 1:2690655/1‑69 (MQ=255) cTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc > 1:1301265/1‑69 (MQ=255) cTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc > 1:439813/1‑69 (MQ=255) cTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc > 1:320986/1‑69 (MQ=255) tGACGCTCACGACTTACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc < 1:1974217/68‑1 (MQ=255) tGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc < 1:304324/68‑1 (MQ=255) tGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc < 1:645918/68‑1 (MQ=255) tGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc < 1:1177218/68‑1 (MQ=255) tGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc < 1:3383041/68‑1 (MQ=255) tGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc < 1:3394256/68‑1 (MQ=255) tGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc < 1:610278/68‑1 (MQ=255) tGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc < 1:642028/68‑1 (MQ=255) gCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAAcc < 1:343588/68‑1 (MQ=255) gCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAAcc < 1:896967/68‑1 (MQ=255) gCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAAcc < 1:937258/68‑1 (MQ=255) gCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAAcc < 1:227244/68‑1 (MQ=255) ccTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAAcc < 1:245203/67‑1 (MQ=255) cTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCAt < 1:1250490/69‑1 (MQ=255) cTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCAt < 1:2702942/69‑1 (MQ=255) cACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCg > 1:2607373/1‑69 (MQ=255) cACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCg > 1:2534953/1‑69 (MQ=255) cACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCg > 1:2478419/1‑69 (MQ=255) cACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCg > 1:2792434/1‑69 (MQ=255) cACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCg > 1:3179805/1‑69 (MQ=255) cACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCg > 1:1987622/1‑69 (MQ=255) cACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCg > 1:634031/1‑69 (MQ=255) cACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCg > 1:1777824/1‑69 (MQ=255) cACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCg > 1:1777696/1‑69 (MQ=255) cACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCg > 1:69348/1‑69 (MQ=255) cACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCg > 1:1663880/1‑69 (MQ=255) cACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCg > 1:1379696/1‑69 (MQ=255) aCTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCGCTTg < 1:882277/69‑1 (MQ=255) cTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCGCTTg < 1:2702791/68‑1 (MQ=255) tGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCGCTTGCa < 1:1002307/69‑1 (MQ=255) tGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCGCTTGCa < 1:3184871/69‑1 (MQ=255) tGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCGCTTGCa < 1:932546/69‑1 (MQ=255) tGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCGCTTGCa < 1:136864/69‑1 (MQ=255) tGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCGCTTGCa < 1:1944178/69‑1 (MQ=255) tGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCGCTTGCa < 1:3290615/69‑1 (MQ=255) | TGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCATCGCTTGCA > minE/1325773‑1325866 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |