| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,326,078 | T→C | 39.3% | V311V (GTA→GTG) | wcaK ← | predicted pyruvyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,326,078 | 0 | T | C | 39.3% | 26.0 / 40.1 | 61 | V311V (GTA→GTG) | wcaK | predicted pyruvyl transferase |
| Reads supporting (aligned to +/- strand): ref base T (17/20); new base C (8/16); total (25/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01 | |||||||||||
TACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTT > minE/1326011‑1326143 | tACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTa < 1:2100385/69‑1 (MQ=255)tACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTa < 1:1820560/69‑1 (MQ=255)tACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTa < 1:3413799/69‑1 (MQ=255)tACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTa < 1:1692206/69‑1 (MQ=255) gACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGt > 1:3097797/1‑68 (MQ=255) gACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGt > 1:1716116/1‑68 (MQ=255) gACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTACAGATCGTTGAGTTCATCCATCACTACg > 1:780951/1‑67 (MQ=255) cacaGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAg > 1:1040514/1‑69 (MQ=255) cacaGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAg > 1:115029/1‑69 (MQ=255) cacaGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAg > 1:2098929/1‑69 (MQ=255) cacaGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAg > 1:1773171/1‑69 (MQ=255) cacaGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAg > 1:344189/1‑69 (MQ=255) caGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACTGGCAgg > 1:3411498/1‑68 (MQ=255) caGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACTGGCAGGa > 1:2584421/1‑69 (MQ=255) caGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACTGGCAGGa > 1:2678427/1‑69 (MQ=255) caGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACTGGCAGGa > 1:1904202/1‑69 (MQ=255) caGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACTGGCAGGa > 1:3376463/1‑69 (MQ=255) caGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACTGGCAGGa > 1:324755/1‑69 (MQ=255) caGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACTGGCAGGa > 1:2154873/1‑69 (MQ=255) caGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACTGGCAGGa > 1:354866/1‑69 (MQ=255) ggCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATc > 1:581954/1‑69 (MQ=255) ggCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATc > 1:2454539/1‑69 (MQ=255) ggCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATc > 1:2358183/1‑69 (MQ=255) ggCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATc > 1:2263480/1‑69 (MQ=255) ggCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATc > 1:2756117/1‑69 (MQ=255) ggCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATc > 1:1106398/1‑69 (MQ=255) ccccAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCt < 1:1224084/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:614960/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:3153566/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:321228/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:843287/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:264195/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:444811/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:432367/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:2351789/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:2125686/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:1217851/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:135765/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:1700137/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:2120077/69‑1 (MQ=255) aTTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:1954030/69‑1 (MQ=255) ttttGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCt < 1:1000467/69‑1 (MQ=255) ttttGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCt < 1:1100925/69‑1 (MQ=255) ttttGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCt < 1:1851164/69‑1 (MQ=255) ttttGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCt < 1:3271779/69‑1 (MQ=255) ttttGCCCATTTCCAGATCGTTGAGTTCATCCATCACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:2911411/68‑1 (MQ=255) tCCAGATCGTTGAGTTCATCCATCACTACGTGGAAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTg > 1:1265959/1‑69 (MQ=255) gTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGccac < 1:3032075/69‑1 (MQ=255) gTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGccac < 1:2794606/69‑1 (MQ=255) gTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGccac < 1:2413104/69‑1 (MQ=255) atccatcACCACGTGGTAACGGGCAGGATCGCTGATGTGc < 1:2344464/40‑1 (MQ=255) tccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGgtc < 1:1995769/68‑1 (MQ=255) tccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGgtc < 1:196062/68‑1 (MQ=255) tccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGgtc < 1:311361/68‑1 (MQ=255) tccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGgtc < 1:1777296/68‑1 (MQ=255) tccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGgtc < 1:1481206/68‑1 (MQ=255) tccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGgtc < 1:50275/68‑1 (MQ=255) tccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGgtc < 1:518383/68‑1 (MQ=255) tccatcACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGgtc < 1:1119769/68‑1 (MQ=255) aCTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCttt > 1:2005354/1‑68 (MQ=255) aCTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCttt > 1:3137618/1‑68 (MQ=255) | TACCGACGGTGAGTTCACAGGCCCCCAGAATTTTGCCCATTTCCAGATCGTTGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTT > minE/1326011‑1326143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |