| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 105,854 | A→T | 51.0% | V146V (GTT→GTA) | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 105,854 | 0 | A | T | 51.0% | ‑6.1 / 110.7 | 90 | V146V (GTT→GTA) | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base A (23/21); new base T (18/28); total (41/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01 | |||||||||||
GATCATCGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATT > minE/105788‑105920 | gatCATCGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAAc > 1:2835232/1‑69 (MQ=255)gatCATCGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAAc > 1:2080128/1‑69 (MQ=255)gatCATCGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAAc > 1:1234710/1‑69 (MQ=255)gatCATCGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAAc > 1:1234874/1‑69 (MQ=255)gatCATCGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAAc > 1:1998095/1‑69 (MQ=255)gatCATCGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAAc > 1:1923745/1‑69 (MQ=255)gatCATCGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAAc > 1:2499348/1‑69 (MQ=255)gatCATCGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAAc > 1:603770/1‑69 (MQ=255)gatCATCGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAAc > 1:1402853/1‑69 (MQ=255) catcGCTACCACCGCGTTAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:3121529/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATTGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:2071223/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:2844611/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:830595/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:2363479/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:819675/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:2408871/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:2088789/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:1200799/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:2409517/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:1255889/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:1956592/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:135640/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:1911348/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:1006709/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:2508430/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:2634338/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:1425823/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:2547305/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:2425411/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:1551419/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:1576270/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:3215211/68‑1 (MQ=255) catcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATATCGCCAAACACTTTTACGt < 1:494409/68‑1 (MQ=255) atcGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:2475841/67‑1 (MQ=255) gCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTc > 1:2773515/1‑69 (MQ=255) gCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTc > 1:3236086/1‑69 (MQ=255) gCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTc > 1:3369058/1‑69 (MQ=255) cgcgATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:2276093/56‑1 (MQ=255) gcgATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:1946896/55‑1 (MQ=255) aTAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGt < 1:96906/52‑1 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTaa < 1:3044878/47‑1 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTaa > 1:2136278/1‑47 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTaa < 1:724626/47‑1 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTaa > 1:1415975/1‑47 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTaa > 1:1478731/1‑47 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTa > 1:3050545/1‑46 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:915905/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:2830737/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:623678/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:2869496/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:1548146/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:1578611/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:2474025/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:425141/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:1815695/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:2433578/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:398359/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:2015270/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:2199452/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:2293932/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGtt > 1:3324634/1‑69 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGt > 1:3219327/1‑68 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTTGTCAGGTTGATGGCGt > 1:1278240/1‑68 (MQ=255) aaCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTTACGTTCGTCAGGTTGATGGCGtt > 1:1668173/1‑69 (MQ=255) aaCTTTGATAATGGCAAA‑CAGAACTCCATCTCGCCAAACACTTTaa > 1:1893410/1‑46 (MQ=37) aCTTTGATAATGTCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGtg > 1:576691/1‑67 (MQ=255) aCTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAAAACTTTAACGTTGGTCAGGtt > 1:785137/1‑59 (MQ=255) tttGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAAt < 1:2373364/69‑1 (MQ=255) tttGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAAt < 1:2789369/69‑1 (MQ=255) tttGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAAt < 1:1663379/69‑1 (MQ=255) tttGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAAt < 1:1910410/69‑1 (MQ=255) tttGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAAt < 1:1328573/69‑1 (MQ=255) tttGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAAt < 1:2351977/69‑1 (MQ=255) tttGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAAt < 1:1328531/69‑1 (MQ=255) tttGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAAt < 1:989417/69‑1 (MQ=255) tttGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAAt < 1:243887/69‑1 (MQ=255) tttGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAAt < 1:2576704/69‑1 (MQ=255) tttGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAAt < 1:2674887/69‑1 (MQ=255) gCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAg < 1:2434970/69‑1 (MQ=255) gCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAg < 1:2538148/69‑1 (MQ=255) gCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAg < 1:3012869/69‑1 (MQ=255) gCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAg < 1:982785/69‑1 (MQ=255) gCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGATCAGGTTGATGGCGTTAATCACCACAAAg < 1:2357226/69‑1 (MQ=255) aCCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAg < 1:2448780/65‑1 (MQ=255) tctcGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGaaa > 1:2771006/1‑69 (MQ=255) tctcGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGaaa > 1:1745415/1‑69 (MQ=255) tctcGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGaaa > 1:37712/1‑69 (MQ=255) tctcGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGa < 1:739330/67‑1 (MQ=255) tctcGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGa < 1:2410785/67‑1 (MQ=255) tctcGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGa < 1:2969540/67‑1 (MQ=255) tctcGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGa < 1:2359050/67‑1 (MQ=255) cGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAAccc > 1:226964/1‑69 (MQ=255) cGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAAccc > 1:1140086/1‑69 (MQ=255) ttAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAAccc > 1:1451313/1‑58 (MQ=255) ttAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGAtt > 1:32669/1‑69 (MQ=255) ttAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGAtt > 1:2399409/1‑69 (MQ=255) ttAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGAtt > 1:2296819/1‑69 (MQ=255) | GATCATCGCTACCACCGCGATAACTTTGATAATGGCAAACCAGAACTCCATCTCGCCAAACACTTTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATT > minE/105788‑105920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |