| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,327,112 | C→T | 40.6% | intergenic (‑102/+174) | wcaK ← / ← wzxC | predicted pyruvyl transferase/colanic acid exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,327,112 | 0 | C | T | 40.6% | 4.0 / 18.9 | 32 | intergenic (‑102/+174) | wcaK/wzxC | predicted pyruvyl transferase/colanic acid exporter |
| Reads supporting (aligned to +/- strand): ref base C (13/6); new base T (5/8); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTTTGTTCGTTGCCGCTTTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCGGCATTCAGTGCCTGA > minE/1327049‑1327173 | tgttttgttCGTTGCCGCTTTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAAGACCCGAg > 1:2646659/1‑69 (MQ=255)tgttttgttCGTTGCCGCTTTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAAGACCCGAg > 1:2461383/1‑69 (MQ=255)tgttttgttCGTTGCCGCTTTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAAGACCCGAg > 1:1884126/1‑69 (MQ=255) tGACGCGTCTTATCAGGCCTACAAGATCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCa > 1:3024215/1‑69 (MQ=255) tGACGCGTCTTATCAGGCCTACAAGATCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCa > 1:3194158/1‑69 (MQ=255) tGACGCGTCTTATCAGGCCTACAAGATCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCa > 1:3320817/1‑69 (MQ=255) tGACGCGTCTTATCAGGCCTACAAGATCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCa > 1:1701513/1‑69 (MQ=255) tGACGCGTCTTATCAGGCCTACAAGATCCGAGCACAGAACCGTAGGACGCATAAGGCG‑TTCACGCCGCa > 1:732058/1‑69 (MQ=17) tCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCGGCa < 1:1396881/69‑1 (MQ=255) cAGGCCTACAAGACCCGAGCACAGAACCGTAGTACGGATAAGGCG‑TTCACGCCGCATCCgg < 1:3380825/61‑1 (MQ=255) cAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCgg < 1:1986961/61‑1 (MQ=255) cAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCgg < 1:2043437/61‑1 (MQ=255) cAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCGGCATTc < 1:739906/66‑1 (MQ=255) aGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCgg < 1:2483893/60‑1 (MQ=255) cTACAAGACCCGAGCACAGAGCCGTAGGACGGATAAGGCGTTTTACGCCGCATCCGGCATTCAGTGCCt > 1:646099/1‑69 (MQ=14) cTACAAGACCCGAGCACAGAGCCGTAGGACGGATAAGGCGTTTTACGCCGCATCCGGCATTCAGTGCCt > 1:62155/1‑69 (MQ=14) cTACAAGACCCGAGCACAGAGCCGTAGGACGGATAAGGCGTTTTACGCCGCATCCGGCATTCAGTGCCt > 1:87635/1‑69 (MQ=14) cTACAAGACCCGAGCACAGAGCCGTAGGACGGATAAGGCGTTTTACGCCGCATCCGGCATTCAGTGCCt > 1:920557/1‑69 (MQ=14) cTACAAGACCCGAGCACAGAGCCGTAGGACGGATAAGGCGTTTTACGCCGCATCCGGCATTCAGTGCCt > 1:2562143/1‑69 (MQ=14) cTACAAGACCCGAGCACAGAGCCGTAGGACGGATAAGGCGTTTTACGCCGCATCCGGCATTCAGTGCCt > 1:2889840/1‑69 (MQ=14) cTACAAGACCCGAGCACAGAGCCGTAGGACGGATAAGGCGTTTTACGCCGCATCCGGCATTCAGTGCCt > 1:2768978/1‑69 (MQ=14) cTACAAGACCCGAGCACAGAGCCGTAGGACGGATAAGGCGTTTTACGCCGCATCCGGCATTCAGTGCCt > 1:1074142/1‑69 (MQ=14) cTACAAGACCCGAGCACAGAGCCGTAGGACGGATAAGGCGTTTTACGCCGCATCCGGCATTCAGTGCCt > 1:2527897/1‑69 (MQ=14) cTACAAGACCCGAGCACAGAGCCGTAGGACGGATAAGGCGTTTTACGCCGCATCCGGCATTCAGTGCCt > 1:149458/1‑69 (MQ=14) tACAAGATCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCGGCATTCAGTGCCTGa < 1:3135804/69‑1 (MQ=255) tACAAGATCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCGGCATTCAGTGCCTGa < 1:2561272/69‑1 (MQ=255) tACAAGATCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCGGCATTCAGTGCCTGa < 1:60414/69‑1 (MQ=255) tACAAGATCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCGGCATTCAGTGCCTGa < 1:1877043/69‑1 (MQ=255) tACAAGATCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCGGCATTCAGTGCCTGa < 1:1821625/69‑1 (MQ=255) tACAAGATCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCGGCATTCAGTGCCTGa < 1:1767146/69‑1 (MQ=255) tACAAGATCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCGGCATTCAGTGCCTGa < 1:164430/69‑1 (MQ=255) tACAAGATCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCGGCATTCAGTGCCTGa < 1:1151189/69‑1 (MQ=255) | TGTTTTGTTCGTTGCCGCTTTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCG‑TTCACGCCGCATCCGGCATTCAGTGCCTGA > minE/1327049‑1327173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |