| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 105,914 | G→T | 45.9% | P126P (CCC→CCA) | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 105,914 | 0 | G | T | 45.9% | 9.2 / 35.2 | 37 | P126P (CCC→CCA) | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base G (6/14); new base T (8/9); total (14/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01 | |||||||||||
TTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAA > minE/105852‑105981 | ttAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGAtt > 1:2399409/1‑69 (MQ=255)ttAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGAtt > 1:2296819/1‑69 (MQ=255)ttAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGAtt > 1:32669/1‑69 (MQ=255) gTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCg < 1:2451955/68‑1 (MQ=255) ttGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGa < 1:282046/69‑1 (MQ=255) tGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGa < 1:2263090/68‑1 (MQ=255) aGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAg < 1:1032598/69‑1 (MQ=255) aGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAg < 1:2795848/69‑1 (MQ=255) aGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAg < 1:2646184/69‑1 (MQ=255) aGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAg < 1:2511577/69‑1 (MQ=255) aGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAg < 1:3414744/69‑1 (MQ=255) ggCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGaa < 1:2422228/68‑1 (MQ=255) ggCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGaa < 1:2464802/68‑1 (MQ=255) ggCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGaa < 1:283666/68‑1 (MQ=255) gTTGATCACCACAAAGAACACGGCGGCAGAAACCCAGGTTGGGAtt > 1:417043/1‑46 (MQ=38) gTTGATCACCACAAAGAACACGGCGGCAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTa > 1:2116656/1‑69 (MQ=255) gTTGATCACCACAAAGAACACGGCGGCAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTa > 1:2349318/1‑69 (MQ=255) gTTGATCACCACAAAGAACACGGCGGCAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTa > 1:3061604/1‑69 (MQ=255) gTTGATCACCACAAAGAACACGGCGGCAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTa > 1:1608714/1‑69 (MQ=255) gTTGATCACCACAAAGAACACGGCGGCAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTa > 1:2618919/1‑69 (MQ=255) gTTGATCACCACAAAGAACACGGCGGCAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTa > 1:621439/1‑69 (MQ=255) gTTGATCACCACAAAGAACACGGCGGCAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTa > 1:873381/1‑69 (MQ=255) aaTCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTAttt > 1:1650976/1‑69 (MQ=255) caccacAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTAcc < 1:3192238/69‑1 (MQ=255) accacAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGa > 1:1427141/1‑45 (MQ=255) accacAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCC‑GATACCAGAACTGAATGTATTTACCCa > 1:1593042/1‑69 (MQ=255) ggcgggAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGtc < 1:2346022/64‑1 (MQ=255) ggcggcAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGtc < 1:2725664/64‑1 (MQ=255) ggcggcAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGtc < 1:2477154/64‑1 (MQ=255) ggcggcAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGtc < 1:2413475/64‑1 (MQ=255) ggcggcAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGtc < 1:339123/64‑1 (MQ=255) ggcggcAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGtc < 1:2082547/64‑1 (MQ=255) ggcggcAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGtc < 1:804151/64‑1 (MQ=255) ggcggcAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGtc < 1:1542509/64‑1 (MQ=255) gcggcAGAAACCCAGGTTGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGtc < 1:690997/63‑1 (MQ=255) aaCCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGc < 1:1766620/69‑1 (MQ=255) aaCCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGc < 1:718004/69‑1 (MQ=255) ggggATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTaaaa > 1:2203631/1‑68 (MQ=255) | TTAACGTTGGTCAGGTTGATGGCGTTAATCACCACAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAA > minE/105852‑105981 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |