| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,332,182 | A→G | 44.4% | A315A (GCT→GCC) | cpsB ← | mannose‑1‑phosphate guanyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,332,182 | 0 | A | G | 44.4% | 11.7 / 57.6 | 72 | A315A (GCT→GCC) | cpsB | mannose‑1‑phosphate guanyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (26/14); new base G (12/20); total (38/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.20e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGG > minE/1332117‑1332246 | aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCa < 1:242577/69‑1 (MQ=255)aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCa < 1:246823/69‑1 (MQ=255)aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCa < 1:3174710/69‑1 (MQ=255)aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCa < 1:3238005/69‑1 (MQ=255)aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCa < 1:1309103/69‑1 (MQ=255)aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCa < 1:1310401/69‑1 (MQ=255)aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCa < 1:3295030/69‑1 (MQ=255)aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCa < 1:1332024/69‑1 (MQ=255)aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCa < 1:3363261/69‑1 (MQ=255)aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCa < 1:451879/69‑1 (MQ=255)aCCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCa < 1:1447924/69‑1 (MQ=255) tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGCCGGTGGTGACCAGGCCAGATTCAGCATaca > 1:2173841/1‑67 (MQ=255) tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATaca > 1:570908/1‑67 (MQ=255) tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATaca > 1:1497621/1‑67 (MQ=255) tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATaca > 1:1396865/1‑67 (MQ=255) tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATaca > 1:1730724/1‑67 (MQ=255) tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATaca > 1:677271/1‑67 (MQ=255) tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATaca > 1:72466/1‑67 (MQ=255) tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATaca > 1:2812765/1‑67 (MQ=255) tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATaca > 1:979084/1‑67 (MQ=255) tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATaca > 1:1056690/1‑67 (MQ=255) tCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATa > 1:3033337/1‑65 (MQ=255) tCTTTGGTCTGCACCACTACCAGATCTCTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATaca > 1:688102/1‑67 (MQ=255) tCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTa < 1:3310594/69‑1 (MQ=255) cTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGAtt > 1:1070326/1‑67 (MQ=255) cTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTa > 1:1153550/1‑68 (MQ=255) cTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTa > 1:2860756/1‑68 (MQ=255) cTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTa > 1:1817981/1‑68 (MQ=255) cTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTa > 1:465881/1‑68 (MQ=255) cTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTa > 1:2501133/1‑68 (MQ=255) cAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCa < 1:2707866/63‑1 (MQ=255) cAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCa < 1:2731328/63‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:3349373/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:3339547/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:1676947/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:1879754/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:1586155/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:1416056/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:3250367/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:1302209/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:1857478/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:887591/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:3216439/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:1874733/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:3164921/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:2994381/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:2354326/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:2029975/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:2556618/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:2568547/46‑1 (MQ=255) gCCAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:1895147/46‑1 (MQ=255) ccAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc < 1:755074/45‑1 (MQ=255) ccAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGccc > 1:2880747/1‑69 (MQ=255) ccAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGccc > 1:417395/1‑69 (MQ=255) ccAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGccc > 1:145579/1‑69 (MQ=255) ccAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGccc > 1:2017526/1‑69 (MQ=255) ccAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGccc > 1:605054/1‑69 (MQ=255) cAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCATGGCAAACGTTGccgt > 1:2884101/1‑67 (MQ=255) cAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCATGGCAAACGTTGccc > 1:282730/1‑68 (MQ=255) cAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCATGGCAAACGTTGccc > 1:2888048/1‑68 (MQ=255) cAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCATGGCAAACGTTGCCCt > 1:891927/1‑69 (MQ=255) cAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCATGGCAAACGTTGCCCt > 1:894413/1‑69 (MQ=255) cAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCATGGCAAACGTTGCCCt > 1:1245/1‑69 (MQ=255) cAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCATGGCAAACGTTGCCCt > 1:2256002/1‑69 (MQ=255) cAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCATGGCAAACGTTGCCCt > 1:1317233/1‑69 (MQ=255) cAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCATGGCAAACGTTGCCCt > 1:590602/1‑69 (MQ=255) cAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCATGGCAAACGTTGCCCt > 1:372471/1‑69 (MQ=255) cAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCATGGCAAACGTTGCCCt > 1:3360490/1‑69 (MQ=255) cAGATTCGGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCATGGCAAACGTTGCCCt > 1:2127714/1‑69 (MQ=255) ttCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGtt > 1:2638752/1‑60 (MQ=255) ttCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTcgg > 1:322672/1‑68 (MQ=255) ttCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGAGGCAAACGTTGCCCTcgg > 1:2584829/1‑68 (MQ=255) | ACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATCACATCGCCGTGGCAAACGTTGCCCTCGG > minE/1332117‑1332246 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |