| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,334,062 | C→T | 51.5% | P97P (CCG→CCA) | wcaI ← | predicted glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,334,062 | 0 | C | T | 51.5% | ‑3.4 / 58.9 | 66 | P97P (CCG→CCA) | wcaI | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base C (15/17); new base T (22/12); total (37/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAACAGCGTTGGCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTT > minE/1334001‑1334128 | aaaaCAGCGTTGGCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGgaaaga < 1:1337793/69‑1 (MQ=255)aaaaCAGCGTTGGCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGgaaaga < 1:2741360/69‑1 (MQ=255)aaaaCAGCGTTGGCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGgaaaga < 1:2452683/69‑1 (MQ=255) aaaCAGCGTTGGCACCACGCCGATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGTGgaaaga < 1:3251826/68‑1 (MQ=255) aaaCAGCGTTGGCACCACGCCGATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGTGgaaaga < 1:1891151/68‑1 (MQ=255) aaaCAGCGTTGGCACCACGCCGATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGTGgaaaga < 1:574888/68‑1 (MQ=255) aaaCAGCGTTGGCACCACGCCGATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGTGgaaaga < 1:1965818/68‑1 (MQ=255) aaaCAGCGTTGGCACCACGCCGATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGTGgaaaga < 1:2096086/68‑1 (MQ=255) aaaCAGCGTTGGCACCACGCCGATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGTGgaaaga < 1:784418/68‑1 (MQ=255) aaaCAGCGTTGGCACCACGCCGATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGTGgaaaga < 1:111062/68‑1 (MQ=255) aaaCAGCGTTGGCACCACGCCGATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGTGgaaaga < 1:330252/68‑1 (MQ=255) aaaCAGCGTTGGCACCACGCCGATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGTGgaaaga < 1:28547/68‑1 (MQ=255) aaaCAGCGTTGGCACCACGCCGATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGTGgaaaga < 1:3274524/68‑1 (MQ=255) aaaCAGCGTTGGCACCACGCCGATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGTGgaaaga < 1:1039907/68‑1 (MQ=255) aaaCAGCGTTGGCACCACGCCGATAATGCGATCCGGCTTCAAGCGACGTTGCGCCATCAGTGgaaaga < 1:1384024/68‑1 (MQ=255) cGTTGGCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAActgct < 1:2567972/69‑1 (MQ=255) cGTTGGCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAActgct < 1:2163858/69‑1 (MQ=255) cGTTGGCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAActgct < 1:2000801/69‑1 (MQ=255) cGTTGGCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAActgct < 1:928249/69‑1 (MQ=255) cGTTGGCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAActgct < 1:1678115/69‑1 (MQ=255) tGGCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAActgct < 1:752453/66‑1 (MQ=255) ggCGCCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGag > 1:1008239/1‑67 (MQ=255) ggCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGAc > 1:3147159/1‑68 (MQ=255) ggCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGAc > 1:1489018/1‑68 (MQ=255) ggCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGAc > 1:3107435/1‑68 (MQ=255) ggCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGAc > 1:801333/1‑68 (MQ=255) ggCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGAc > 1:2306986/1‑68 (MQ=255) ggCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGAc > 1:1242975/1‑68 (MQ=255) ggCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGAc > 1:1246909/1‑68 (MQ=255) ggCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGAc > 1:60636/1‑68 (MQ=255) ggCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAA‑AAACTGCTGACg > 1:1060575/1‑68 (MQ=255) ccacGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGACGGCaa < 1:3089190/69‑1 (MQ=255) ccacGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGACGGCaa < 1:1087869/69‑1 (MQ=255) cacGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGgaaaga > 1:2179204/1‑53 (MQ=255) cacGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGgaaaga > 1:405342/1‑53 (MQ=255) cacGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGgaaaga > 1:1220885/1‑53 (MQ=255) cacGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGgaaaga > 1:2527552/1‑53 (MQ=255) cacGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGACGGCaaa < 1:2196282/69‑1 (MQ=255) taatGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGACGGCAAAACTGCCCa < 1:147874/69‑1 (MQ=255) taatGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGACGGCAAAACTGCCCa < 1:800198/69‑1 (MQ=255) ccGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGAc > 1:374681/1‑46 (MQ=255) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:3313874/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:523646/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:78559/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:239929/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:3388568/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:3426277/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:3051850/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:1013933/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:1109605/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:1142954/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:1189074/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:1317333/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:1344264/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:1352055/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:1692939/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:2072716/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:2285118/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:2423215/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:2579928/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:2718372/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:2969640/1‑51 (MQ=39) ccAGCGACGTTGCGCCATCAGTGGAAAGAAACTGCTGACGGCAAAACTGccg > 1:3165349/1‑51 (MQ=39) aGCGGAAAGAAACTGCTGACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGtttt < 1:1431587/69‑1 (MQ=255) aGCGGAAAGAAACTGCTGACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGtttt < 1:794779/69‑1 (MQ=255) gCGGAAAGAAACTGCTGACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGtttt < 1:1085984/68‑1 (MQ=255) | AAAACAGCGTTGGCACCACGCCAATAATGCGATCCGGCTTCCAGCGACGTTGCGCCATCAGCGGAAAGAAACTGCTGACGGCAAAACTGCCCAGATGCAACAGGCGTTTCAGGGTGCTCGGCTGTTTT > minE/1334001‑1334128 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |