| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,334,438 | A→G | 24.2% | S131P (TCG→CCG) | nudD ← | GDP‑mannose mannosyl hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,334,438 | 0 | A | G | 24.2% | 84.4 / 24.0 | 74 | S131P (TCG→CCG) | nudD | GDP‑mannose mannosyl hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (35/21); new base G (11/7); total (46/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-01 | |||||||||||
AAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAC > minE/1334385‑1334498 | aaaaTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTa < 1:502303/69‑1 (MQ=255) aGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGt < 1:1001904/69‑1 (MQ=255) aGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGt < 1:1988604/69‑1 (MQ=255) aGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGt < 1:724032/69‑1 (MQ=255) aaCATTATCACTGGCGCGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:3288847/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGc > 1:983981/1‑67 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:552811/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:641162/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:3373542/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:3349906/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:1038860/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:3195427/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:3159793/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:3135166/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:2972497/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:2317814/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:2080432/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:1739378/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:1551795/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:1437673/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCa > 1:1085484/1‑68 (MQ=255) aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTAATGCTGCTCATCCGGCa > 1:1128183/1‑68 (MQ=255) cATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGt < 1:2523121/68‑1 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACa > 1:2820847/1‑67 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:1319184/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:2972164/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:1270234/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:3018909/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:481572/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:3118537/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:1151478/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:2511104/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:2417774/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:112998/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:896679/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:3158138/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:945119/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:3258425/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGAAACAGc > 1:3378093/1‑69 (MQ=255) tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAG‑GGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc > 1:225798/1‑68 (MQ=255) cACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:498710/68‑1 (MQ=255) cACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:2896472/68‑1 (MQ=255) cACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:2505350/68‑1 (MQ=255) cACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:2102558/68‑1 (MQ=255) cACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:95148/68‑1 (MQ=255) tGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:3317063/65‑1 (MQ=255) tGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:1167951/65‑1 (MQ=255) tGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:1653177/65‑1 (MQ=255) tGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:920669/65‑1 (MQ=255) tGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:2869623/65‑1 (MQ=255) tGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:2333093/65‑1 (MQ=255) tGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:2659914/65‑1 (MQ=255) tGGCGAGCAGCGCGTCCGACGTCAGCCAACGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:65861/65‑1 (MQ=255) ggCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:2225593/64‑1 (MQ=255) gAGCAACGCGTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTcatctt > 1:2964729/1‑68 (MQ=255) gAGCAACGCGTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCatcttc > 1:531452/1‑69 (MQ=255) gAGCAACGCGTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCatcttc > 1:1073927/1‑69 (MQ=255) gAGCAACGCGTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCatcttc > 1:2131622/1‑69 (MQ=255) gAGCAACGCGTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCatcttc > 1:2224605/1‑69 (MQ=255) gAGCAACGCGTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCatcttc > 1:802088/1‑69 (MQ=255) gAGCAACGCGTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCatcttc > 1:1582876/1‑69 (MQ=255) gAGCAACGCGTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCatcttc > 1:2789668/1‑69 (MQ=255) gAGCAACGCGTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCatcttc > 1:411628/1‑69 (MQ=255) gAGCAACGCGTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCatcttc > 1:400577/1‑69 (MQ=255) gAGCAACGCGTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCatcttc > 1:292608/1‑69 (MQ=255) agcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTcc < 1:289626/69‑1 (MQ=255) cgcgTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCATCTTCCGCTAc < 1:1359736/69‑1 (MQ=255) cgcgTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCATCTTCCGCTAc < 1:2670075/69‑1 (MQ=255) cgcgTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCATCTTCCGCTAc < 1:806554/69‑1 (MQ=255) cgcgTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCATCTTCCGCTAc < 1:873079/69‑1 (MQ=255) cgcgTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCATCTTCCGCTAc < 1:3126667/69‑1 (MQ=255) cgcgTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCATCTTCCGCTAc < 1:1736024/69‑1 (MQ=255) cgcgTCCGGCGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCATCTTCCGCTAc < 1:1366034/69‑1 (MQ=255) cgTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGc < 1:1650504/53‑1 (MQ=255) | AAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAC > minE/1334385‑1334498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |