| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,336,186 | C→T | 52.2% | Q245Q (CAG→CAA) | gmd ← | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,336,186 | 0 | C | T | 52.2% | 3.8 / 100.0 | 111 | Q245Q (CAG→CAA) | gmd | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (48/5); new base T (35/23); total (83/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
CTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGG > minE/1336119‑1336253 | ctgcCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCt > 1:2345504/1‑69 (MQ=255)ctgcCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCt > 1:709602/1‑69 (MQ=255)ctgcCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCt > 1:664442/1‑69 (MQ=255)ctgcCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCt > 1:3407229/1‑69 (MQ=255)ctgcCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCt > 1:1120160/1‑69 (MQ=255)ctgcCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCt > 1:298636/1‑69 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:3036811/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:1606267/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:2294687/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:2385196/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:2417733/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:2721018/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:2732954/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:2848591/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:2941095/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:3024521/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:1021443/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:3067955/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:3166240/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:550051/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:783760/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:88117/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:911824/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:104465/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:1322685/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:1362853/69‑3 (MQ=255) tttCCACGAACTGACGTACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCctgtt < 1:1421543/69‑3 (MQ=255) tCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCtgctgc > 1:1101166/1‑69 (MQ=255) tCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCtgctgc > 1:2730497/1‑69 (MQ=255) tACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGcat > 1:1037333/1‑53 (MQ=255) tACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCAttttt > 1:977929/1‑69 (MQ=255) tACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCAttttt > 1:378781/1‑69 (MQ=255) tACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCAttttt > 1:3166109/1‑69 (MQ=255) tACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCAttttt > 1:314263/1‑69 (MQ=255) tACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCAttttt > 1:2071620/1‑69 (MQ=255) tACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCAttttt > 1:2031753/1‑69 (MQ=255) tACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCAttttt > 1:1647859/1‑69 (MQ=255) tACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCAttttt > 1:1921138/1‑69 (MQ=255) tACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTACTGCTGCAGCATCATCCACTGCAtttt > 1:531428/1‑68 (MQ=255) aCTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:2410148/1‑69 (MQ=255) aCTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGATGCAGCATCATCCACTGCATTTTTa > 1:1272983/1‑69 (MQ=255) cTGAACGCCGGTCGCTATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:1067639/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTcc > 1:2469272/1‑40 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:1977701/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:1054202/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:1826667/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:2498642/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:828927/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:606988/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:602441/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:3304775/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:1576770/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:3367721/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:1626833/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:2067371/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:1009964/1‑68 (MQ=255) cTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTa > 1:3432069/1‑68 (MQ=255) gAACGCCGGTCGCGATGACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:1315328/1‑50 (MQ=38) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:400172/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:1326003/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:1055194/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:1107660/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:3396230/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:413680/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:2734282/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:1985127/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:2249698/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:2565352/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:2561392/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:1484333/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:1519559/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTCCTGTTGCAGcatc > 1:720971/1‑50 (MQ=39) gAACGCCGGTCGCGATAACGAAATCTTCCGGTTGTTACTGTTGCAGcatc > 1:2603405/1‑50 (MQ=255) aaCGCCGGTCGCGATAACGGAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGt > 1:3217244/1‑68 (MQ=255) aaCGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTa > 1:3239341/1‑69 (MQ=255) ccGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTgca > 1:1553747/1‑40 (MQ=255) cgcgATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGc > 1:2278531/1‑69 (MQ=255) cgcgATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGc > 1:2668992/1‑69 (MQ=255) cgcgATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGc > 1:2161448/1‑69 (MQ=255) cgcgATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGc > 1:886167/1‑69 (MQ=255) cgcgATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGc > 1:1923165/1‑69 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCAtttta > 1:801630/1‑44 (MQ=38) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGcccc > 1:1545059/1‑67 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1932457/1‑68 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1580017/1‑68 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1727482/1‑68 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:3342658/1‑68 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:3291667/1‑68 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1803991/1‑68 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1312983/1‑68 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1828488/1‑68 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:245572/1‑68 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:2202117/1‑68 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:2060917/1‑68 (MQ=255) aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACAGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1833324/1‑68 (MQ=255) gCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAggg < 1:3247262/69‑1 (MQ=255) gCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAggg < 1:1777576/69‑1 (MQ=255) gCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAggg < 1:145022/69‑1 (MQ=255) gCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAggg < 1:2160356/69‑1 (MQ=255) gCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAggg < 1:1927089/69‑1 (MQ=255) | CTGCCGCCATTTCCACGAACTGACGCACGGAGTACTGAACGCCGGTCGCGATAACGAAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGG > minE/1336119‑1336253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |