| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,336,234 | G→A | 36.1% | H229H (CAC→CAT) | gmd ← | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,336,234 | 0 | G | A | 36.1% | 72.6 / 61.9 | 105 | H229H (CAC→CAT) | gmd | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (20/47); new base A (15/23); total (35/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
AAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGA > minE/1336175‑1336298 | aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGcccc > 1:1545059/1‑67 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1727482/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:3423282/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:2202117/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:2060917/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:245572/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1932457/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:3342658/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:3291667/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1828488/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1803991/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1731790/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1580017/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1312983/1‑68 (MQ=255)aaaTCTTCCGGTTGTTCCTGTTGCAGCATCATCCACAGCATTTTTACGTAGTCTTTGGCATGGCCCCa > 1:1833324/1‑68 (MQ=255) gCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAggg < 1:3247262/69‑1 (MQ=255) gCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAggg < 1:1777576/69‑1 (MQ=255) gCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAggg < 1:1927089/69‑1 (MQ=255) gCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAggg < 1:145022/69‑1 (MQ=255) gCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAggg < 1:2160356/69‑1 (MQ=255) ccTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:2900276/69‑1 (MQ=255) ccTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGGCACGCAGGGAATCCa < 1:225066/69‑1 (MQ=255) tgctgcAGCATCATCTACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:1198963/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:3020657/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:228446/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:2274356/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:2952098/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:760426/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:1837841/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:2346367/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:1146580/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:1149917/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:2341379/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:3287867/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:604123/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:1541832/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:2325403/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:1413921/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCat < 1:1374370/68‑1 (MQ=255) tgctgcAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCAAGTCACGCAGGGAATCCat < 1:752472/68‑1 (MQ=255) gcaTCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCg < 1:205409/68‑1 (MQ=255) tcatcCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAgg > 1:1441782/1‑68 (MQ=255) atcCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATAtt > 1:2412089/1‑59 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGTCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:1058274/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:3158560/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:3413190/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:3058565/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:2467002/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:3212460/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:2615304/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:328318/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:2263887/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:447246/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:1986081/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:471411/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:506701/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:522428/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:567876/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:611549/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:1060875/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:1338470/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:755843/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:1107242/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGATACAGGCAc < 1:775138/69‑1 (MQ=255) tGCATTTTTACGTAGTCTTTGGCATGGCCCCAGTCACGAAGGGAATCCATATTGCCGAGATACAGGCAc < 1:423187/69‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGTAATCCa < 1:441793/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:702772/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:96818/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:3221073/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:1034537/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:2873817/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:1029254/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:1111436/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:1137526/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:1240983/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:1342251/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:1441820/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:1573481/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:1983647/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:2268372/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:3166405/48‑1 (MQ=255) gCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:2481352/48‑1 (MQ=255) cATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCa < 1:791278/47‑1 (MQ=255) cATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGa > 1:623432/1‑69 (MQ=255) cATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGa > 1:3268541/1‑69 (MQ=255) cATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGa > 1:2481454/1‑69 (MQ=255) tttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:1593857/1‑63 (MQ=255) tttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:1869392/1‑63 (MQ=255) tttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:332053/1‑63 (MQ=255) tttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:2322382/1‑63 (MQ=255) tttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:694349/1‑63 (MQ=255) tttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:69961/1‑63 (MQ=255) tttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:503904/1‑63 (MQ=255) tttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:2496362/1‑63 (MQ=255) tttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:2640169/1‑63 (MQ=255) tttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:1062526/1‑63 (MQ=255) tttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:2326231/1‑63 (MQ=255) tttACGTAGTCTTTGGCGTGG‑‑CCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:240496/1‑61 (MQ=255) tttACGTAGTCTTTGGAGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCAc > 1:2041943/1‑63 (MQ=255) tGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACATGCACGACTCCAGCCCCTGGGCGa > 1:1830493/1‑69 (MQ=255) | AAATCTTCCGGCTGTTCCTGCTGCAGCATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGA > minE/1336175‑1336298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |