| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,336,510 | A→G | 49.4% | Y137Y (TAT→TAC) | gmd ← | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,336,510 | 0 | A | G | 49.4% | ‑6.1 / 71.9 | 77 | Y137Y (TAT→TAC) | gmd | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (20/19); new base G (13/25); total (33/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCC > minE/1336444‑1336576 | gACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:1062748/69‑1 (MQ=255)gACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:696065/69‑1 (MQ=255)gACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:3367114/69‑1 (MQ=255)gACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:3033870/69‑1 (MQ=255)gACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:2987160/69‑1 (MQ=255)gACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:2721312/69‑1 (MQ=255)gACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:2647213/69‑1 (MQ=255)gACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:2622958/69‑1 (MQ=255)gACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATa < 1:1369635/69‑1 (MQ=255) cGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCaga > 1:640465/1‑69 (MQ=255) cGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCaga > 1:1793300/1‑69 (MQ=255) cGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCaga > 1:1976396/1‑69 (MQ=255) cGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCag > 1:1594681/1‑68 (MQ=255) cgcgGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGaa > 1:1510018/1‑69 (MQ=255) cgcgGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGaa > 1:401164/1‑69 (MQ=255) cgcgGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGaa > 1:843180/1‑69 (MQ=255) gggTAGAACGGCGTGGTCTC‑‑TCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:3087840/69‑1 (MQ=255) gTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:98572/69‑1 (MQ=255) gTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:883562/69‑1 (MQ=255) gTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:908440/69‑1 (MQ=255) gTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:561376/69‑1 (MQ=255) gTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:2141339/69‑1 (MQ=255) gTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:111895/69‑1 (MQ=255) gTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:1598256/69‑1 (MQ=255) gTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:154838/69‑1 (MQ=255) tAGAACGGCGTTGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:2314412/68‑1 (MQ=255) tAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:1372668/68‑1 (MQ=255) tAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:709343/68‑1 (MQ=255) tAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:1333200/68‑1 (MQ=255) tAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:324092/68‑1 (MQ=255) tAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:2535005/68‑1 (MQ=255) aGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:1821269/67‑1 (MQ=255) tCTGCGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTg < 1:2338226/49‑1 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCa > 1:3207854/1‑68 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:2610666/1‑69 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:3394482/1‑69 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:1386301/1‑69 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:3413038/1‑69 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:343573/1‑69 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:618168/1‑69 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:818721/1‑69 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:2098260/1‑69 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:1268126/1‑69 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:916488/1‑69 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:541717/1‑69 (MQ=255) cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTATTTTCCAg > 1:2670077/1‑69 (MQ=255) cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:604137/55‑1 (MQ=255) cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:621964/55‑1 (MQ=255) cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:522220/55‑1 (MQ=255) cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:2718904/55‑1 (MQ=255) cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:1066147/55‑1 (MQ=255) cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:3275044/55‑1 (MQ=255) cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:2973951/55‑1 (MQ=255) cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:262926/55‑1 (MQ=255) cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:2617225/55‑1 (MQ=255) cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:1804268/55‑1 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCcagacaga > 1:143084/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:3292932/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:3264417/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:3150901/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:2296820/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:2096032/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:18335/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:699259/1‑67 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:712933/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:3027138/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:709020/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:1047191/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:450914/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:3231928/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:1235791/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:2981961/69‑1 (MQ=255) cATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGcc > 1:734163/1‑68 (MQ=255) cATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGcc > 1:2621648/1‑68 (MQ=255) cATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGcc > 1:252337/1‑68 (MQ=255) cATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGcc > 1:2244345/1‑68 (MQ=255) cATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGcc > 1:1374276/1‑68 (MQ=255) | GACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCC > minE/1336444‑1336576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |