| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 106,094 | C→T | 72.7% | L66L (CTG→CTA) | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 106,094 | 0 | C | T | 72.7% | 75.5 / 29.0 | 73 | L66L (CTG→CTA) | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base C (11/9); new base T (19/34); total (30/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
CCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGC > minE/106031‑106157 | ccAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTg < 1:1232346/69‑1 (MQ=255) aGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTg < 1:2528664/57‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:2350698/69‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:3212050/69‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:2071360/69‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:1533180/69‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:1463506/69‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:212345/69‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:290953/69‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:554447/69‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:1240875/69‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:2491077/69‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:781421/69‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:1160444/69‑1 (MQ=255) cAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCTAGCTGACGCATGATCAGaa < 1:2317411/69‑1 (MQ=255) tGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGc > 1:127260/1‑68 (MQ=255) ggCTAAAGGAGCCTGCGACAGGTTCTTCGCCCACCATTTCACCCAGCTGACGCATGATCAGAAAGGc > 1:1680934/1‑67 (MQ=255) ggCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCg > 1:2721557/1‑68 (MQ=255) ggCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCg > 1:3323967/1‑68 (MQ=255) ggCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCg > 1:1488725/1‑68 (MQ=255) ggCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCg > 1:934510/1‑68 (MQ=255) ggCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCg > 1:2096631/1‑68 (MQ=255) ggCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCg > 1:2951654/1‑68 (MQ=255) ggCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCg > 1:1922506/1‑68 (MQ=255) aGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTg < 1:1838021/43‑1 (MQ=255) ggAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAcc > 1:977258/1‑69 (MQ=255) ggAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAcc > 1:975581/1‑69 (MQ=255) gCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc < 1:321839/69‑1 (MQ=255) gCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc < 1:3238415/69‑1 (MQ=255) gCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc < 1:2865807/69‑1 (MQ=255) gCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc < 1:555357/69‑1 (MQ=255) gCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc < 1:1189702/69‑1 (MQ=255) gCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc < 1:2402817/69‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:3056900/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:2946373/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:3243939/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:3278875/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:457659/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:272721/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:59390/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:1053930/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:1218553/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:1829678/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:2371888/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:1963366/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:1443058/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:1417561/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:1369079/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:2210392/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:1196817/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:225792/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:1023595/68‑1 (MQ=255) accaccATTTCACCTAGCTGACGCATGATCAGAAAGGAGATAAAACCAGCAATGGCGTAAACCAGGAt < 1:571299/68‑1 (MQ=255) caccaTTTCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:960183/66‑1 (MQ=255) tCACCTAGCTGACGCATGATCAGAAGGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTgg > 1:3048116/1‑68 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt > 1:3352979/1‑59 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTgg > 1:1185890/1‑68 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:67060/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:3234856/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:1136193/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:1713474/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:1736841/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:1099553/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:3140701/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:2317031/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:2465475/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:246858/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:2530115/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:2543282/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:2207139/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:290155/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:2195819/1‑69 (MQ=255) tCACCTAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGc > 1:2928722/1‑69 (MQ=255) cAGCTGACGCATGTTCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:2903647/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCTATAAAACCAGCAATGGCGTAACCCAGGAt < 1:773306/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:3370666/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:534067/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:1269864/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:2233073/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:2705719/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:802740/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:831257/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:867151/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:2247268/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:2086176/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:2067030/54‑1 (MQ=255) cAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:1806251/54‑1 (MQ=255) | CCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGC > minE/106031‑106157 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |