| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,336,543 | A→G | 32.7% | T126T (ACT→ACC) | gmd ← | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,336,543 | 0 | A | G | 32.7% | 48.2 / 32.4 | 64 | T126T (ACT→ACC) | gmd | GDP‑D‑mannose dehydratase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (27/16); new base G (13/8); total (40/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGA > minE/1336489‑1336607 | cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCa > 1:3207854/1‑68 (MQ=255)cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:1386301/1‑69 (MQ=255)cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:2098260/1‑69 (MQ=255)cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:618168/1‑69 (MQ=255)cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:541717/1‑69 (MQ=255)cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:343573/1‑69 (MQ=255)cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:3413038/1‑69 (MQ=255)cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:3394482/1‑69 (MQ=255)cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:2610666/1‑69 (MQ=255)cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:818721/1‑69 (MQ=255)cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:1268126/1‑69 (MQ=255)cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAg > 1:916488/1‑69 (MQ=255)cGGAATTTCCTGCACCAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTATTTTCCAg > 1:2670077/1‑69 (MQ=255)cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:3275044/55‑1 (MQ=255)cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:522220/55‑1 (MQ=255)cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:2617225/55‑1 (MQ=255)cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:262926/55‑1 (MQ=255)cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:604137/55‑1 (MQ=255)cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:2718904/55‑1 (MQ=255)cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:2973951/55‑1 (MQ=255)cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:621964/55‑1 (MQ=255)cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:1066147/55‑1 (MQ=255)cGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGa < 1:1804268/55‑1 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCcagacaga > 1:143084/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:2296820/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:18335/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:3292932/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:2096032/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:3150901/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:3264417/1‑67 (MQ=255) tCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGa > 1:699259/1‑67 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:450914/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:709020/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:2981961/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:3027138/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:712933/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:1047191/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:3231928/69‑1 (MQ=255) cAGACCGTACAGTTCAGAAGTAGAAGCCTGATAGAAACGGGTTTTCTTTTCCAGACCGAGGAAGCGGAt < 1:1235791/69‑1 (MQ=255) cATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGcc > 1:1374276/1‑68 (MQ=255) cATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGcc > 1:734163/1‑68 (MQ=255) cATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGcc > 1:2244345/1‑68 (MQ=255) cATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGcc > 1:2621648/1‑68 (MQ=255) cATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGcc > 1:252337/1‑68 (MQ=255) cAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTcc > 1:1042796/1‑67 (MQ=255) cAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTcc > 1:939734/1‑67 (MQ=255) cAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTcc > 1:1346965/1‑67 (MQ=255) cAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTcc > 1:2784155/1‑67 (MQ=255) cAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTcc > 1:2159270/1‑67 (MQ=255) tCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGc > 1:355324/1‑59 (MQ=255) gTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAg > 1:1214560/1‑68 (MQ=255) gTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAg > 1:2891406/1‑68 (MQ=255) gTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAg > 1:1302310/1‑68 (MQ=255) gTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAg > 1:292307/1‑68 (MQ=255) tGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCg < 1:423607/69‑1 (MQ=255) tGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCg < 1:1038460/69‑1 (MQ=255) tGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCg < 1:3252045/69‑1 (MQ=255) ggAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCg < 1:2059708/68‑1 (MQ=255) ggAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGt > 1:2463566/1‑69 (MQ=255) gAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTa < 1:692825/69‑1 (MQ=255) gAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTa < 1:688904/69‑1 (MQ=255) gAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGAAGCGTa < 1:442833/69‑1 (MQ=255) aGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTa < 1:2705623/67‑1 (MQ=255) gAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTc < 1:1989788/69‑1 (MQ=255) aaCGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGa < 1:3172341/69‑1 (MQ=255) aaCGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGa < 1:210278/69‑1 (MQ=255) aaCGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGa < 1:1678435/69‑1 (MQ=255) aaCGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGa > 1:577302/1‑69 (MQ=255) aaCGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGa < 1:530649/69‑1 (MQ=255) aaCGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGa > 1:3234115/1‑69 (MQ=255) aaCGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGa < 1:349642/69‑1 (MQ=255) aaCGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGa > 1:2437731/1‑69 (MQ=255) aaCGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGa < 1:2919738/69‑1 (MQ=255) aaCGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGa < 1:3389183/69‑1 (MQ=255) aGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTAcc > 1:2869921/1‑54 (MQ=255) aGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTAcc > 1:2142498/1‑54 (MQ=255) aGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTAcc > 1:897699/1‑54 (MQ=255) aGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTAcc > 1:2920851/1‑54 (MQ=255) aGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTAcc > 1:2137850/1‑54 (MQ=255) aGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTAcc > 1:2625830/1‑54 (MQ=255) aGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTAcc > 1:2273332/1‑54 (MQ=255) aGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTAcc > 1:2543545/1‑54 (MQ=255) aGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTAca > 1:409173/1‑53 (MQ=255) aGTTTTCTTTTCCACACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTAc > 1:197034/1‑53 (MQ=255) aGTTTTCTTTTCAAGACCGAGGAAGCGGATCGCCTCGAGCAGGCGCAGCGTAc > 1:2518298/1‑53 (MQ=255) | CGGAATTTCCTGCACCAGACCATACAGTTCAGAGGTGGAAGCCTGATAGAAACGAGTTTTCTTTTCCAGACCGAGGAAGCGGATCGCCTCCAGCAGGCGCAGCGTACCCATCGCGTCGA > minE/1336489‑1336607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |