| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,337,984 | C→T | 22.8% | S91S (TCG→TCA) | wcaE ← | predicted glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,337,984 | 0 | C | T | 22.8% | 97.8 / 21.4 | 74 | S91S (TCG→TCA) | wcaE | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base C (20/37); new base T (2/15); total (22/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.68e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 | |||||||||||
ACGTTATCTTTTTGCATTTTTAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTA > minE/1337919‑1338046 | aCGTTATCTTTTTGCATTTTTAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGaa > 1:2262951/1‑69 (MQ=255)aCGTTATCTTTTTGCATTTTTAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGaa > 1:1055983/1‑69 (MQ=255) gCATTTTTAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAaacaac > 1:1577171/1‑68 (MQ=255) gCATTTTTAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAaacaac > 1:1386046/1‑68 (MQ=255) gCATTTTTAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAaacaac > 1:517398/1‑68 (MQ=255) gCATTTTTAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAaacaac > 1:386649/1‑68 (MQ=255) gCATTTTTAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAaacaa > 1:306066/1‑67 (MQ=255) ttttAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGaa < 1:2174220/69‑1 (MQ=255) ttttAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGaa < 1:2447819/69‑1 (MQ=255) ttttAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGaa < 1:2851986/69‑1 (MQ=255) ttttAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGaa < 1:668319/69‑1 (MQ=255) ttttAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGaa < 1:1091059/69‑1 (MQ=255) tAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGaa < 1:802628/66‑1 (MQ=255) aCTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTg < 1:1313588/68‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:2771230/69‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:2505998/69‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:2782034/69‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:2756605/69‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:2746826/69‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:3087067/69‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:2180335/69‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:1023828/69‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:3096682/69‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:1793977/69‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:1499250/69‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:1437758/69‑1 (MQ=255) aTTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:142918/69‑1 (MQ=255) tttGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:1538628/68‑1 (MQ=255) tttGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGc < 1:664830/68‑1 (MQ=255) tccTGATGAAAAATATCGCCTGAATTGAGAAACAGCGCGAACt > 1:2422006/3‑43 (MQ=37) tccTGATGAAAAATATCGACTGAATTGAGAAACAGCGCGAACTTGCCTtg > 1:647211/3‑50 (MQ=255) tCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGaa < 1:2302390/40‑1 (MQ=255) cTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGtt < 1:3099760/69‑1 (MQ=255) cTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGtt < 1:3150088/69‑1 (MQ=255) cTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGtt < 1:3221528/69‑1 (MQ=255) cTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGtt < 1:1050338/69‑1 (MQ=255) cTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGtt > 1:1225801/1‑69 (MQ=255) cTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGtt < 1:2743160/69‑1 (MQ=255) cTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGtt < 1:1768613/69‑1 (MQ=255) cTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGtt < 1:2018488/69‑1 (MQ=255) tgatgaAAAATATCGCCCGAATTGAGAAACAACGCGAACTTg < 1:3068316/42‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACGGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:660185/69‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:816265/69‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:495382/69‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:1730941/69‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:627007/69‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:242891/69‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:1214916/69‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:1119482/69‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:2477654/69‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:2843292/69‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:73127/69‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:2547468/69‑1 (MQ=255) gatgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:2558788/69‑1 (MQ=255) gatgaAAAATATAGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:2223816/69‑1 (MQ=255) atgaAAAATATCGCCTGAATTGAGAAACAGCGCGAACTTGCCTTGCGCCATTTCAATACCTTTGTTCa < 1:3025518/68‑1 (MQ=255) atCGCCCGAATTGAGAGACAACGCGAACTTGCCTTATGCCATCGCAATGCCTTTGTTCATGGCGTCGTa < 1:523982/69‑1 (MQ=255) atCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa < 1:743576/69‑1 (MQ=255) atCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa < 1:3158288/69‑1 (MQ=255) atCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa < 1:3239828/69‑1 (MQ=255) atCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTCCCATCGCAATGCCTTTGTTCATGGCGTCGTa < 1:3379985/69‑1 (MQ=255) tCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa > 1:1284568/1‑68 (MQ=255) tCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa > 1:3368545/1‑68 (MQ=255) tCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa > 1:2036926/1‑68 (MQ=255) tCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa > 1:3092651/1‑68 (MQ=255) tCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa > 1:298016/1‑68 (MQ=255) tCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa > 1:1127848/1‑68 (MQ=255) tCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa > 1:2898201/1‑68 (MQ=255) tCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa > 1:699847/1‑68 (MQ=255) tCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa > 1:2836149/1‑68 (MQ=255) tCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa > 1:2478823/1‑68 (MQ=255) tCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTa > 1:2639101/1‑68 (MQ=255) tCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTCGTTCATGGCGTCGTa > 1:1093552/1‑68 (MQ=255) cGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGtt < 1:3021850/55‑1 (MQ=255) | ACGTTATCTTTTTGCATTTTTAACTTACGGACAAAATTTGCGGCATTCTGATGAAAAATATCGCCCGAATTGAGAAACAACGCGAACTTGCCTTGTGCCATCGCAATGCCTTTGTTCATGGCGTCGTA > minE/1337919‑1338046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |