| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,339,861 | A→G | 30.5% | I272I (ATT→ATC) | wcaC ← | predicted glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,339,861 | 0 | A | G | 30.5% | 77.1 / 40.6 | 85 | I272I (ATT→ATC) | wcaC | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (23/36); new base G (9/17); total (32/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01 | |||||||||||
TTCAAAGCCGTGATTAACCACGTTGCCAGCGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAT > minE/1339795‑1339928 | ttCAAAGCCGTGATTAACCACGTTGCCAGCGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCa > 1:326623/1‑67 (MQ=255)ttCAAAGCCGTGATTAACCACGTTGCCAGCGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAAt > 1:1278472/1‑69 (MQ=255) aTTAACCACGTTGCCAGCGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCa > 1:1224751/1‑68 (MQ=255) aTTAACCACGTTGCCAGCGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAg > 1:1586361/1‑69 (MQ=255) aTTAACAACGTTGCCAGCGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAg > 1:443180/1‑69 (MQ=255) cGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTa < 1:2693189/69‑1 (MQ=255) cGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTa < 1:2885540/69‑1 (MQ=255) cGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTa < 1:2771869/69‑1 (MQ=255) cGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTa < 1:1649917/69‑1 (MQ=255) cGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTa < 1:1492647/69‑1 (MQ=255) cGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTa < 1:1413812/69‑1 (MQ=255) cGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTa < 1:2368874/69‑1 (MQ=255) cGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTa < 1:811581/69‑1 (MQ=255) cGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTa < 1:1194619/69‑1 (MQ=255) cGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTa < 1:232310/69‑1 (MQ=255) gAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAg > 1:2710428/1‑69 (MQ=255) gAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAg > 1:1514855/1‑69 (MQ=255) gAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAg > 1:3328843/1‑69 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:2966518/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:2744914/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:3162967/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:1011143/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:3372693/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:2600901/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:2526104/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:2409273/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:446969/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:2342415/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:983302/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:215852/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:2157958/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:2125855/68‑1 (MQ=255) cGGCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:1615416/68‑1 (MQ=255) ggCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGtt < 1:2865821/67‑1 (MQ=255) gCGAGAACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACGAGtt < 1:2686486/66‑1 (MQ=255) gCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGt > 1:2027649/1‑59 (MQ=255) gaACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGTTGCTGCtt > 1:3065182/1‑69 (MQ=255) gaACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGTTGCTGCtt > 1:502891/1‑69 (MQ=255) gaACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGTTGCTGCtt > 1:1255864/1‑69 (MQ=255) gaACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGTTGCTGCtt > 1:566260/1‑69 (MQ=255) gaACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGTTGCTGCtt > 1:3000634/1‑69 (MQ=255) gaACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGTTGCTGCtt > 1:87173/1‑69 (MQ=255) gaACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGTTGCTGCtt > 1:968607/1‑69 (MQ=255) gaACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCACCAGTTGCTGCtt > 1:2090680/1‑69 (MQ=255) gaACTTACCGAAAGTATGCAGTTCGATTTTGTCGCTCAGCGCCATCATCTCGCGCAACAGTTGCTGCtt > 1:2306874/1‑69 (MQ=255) tATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcgt < 1:3026953/69‑1 (MQ=255) tATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcgt < 1:3023344/69‑1 (MQ=255) tATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcgt < 1:2269770/69‑1 (MQ=255) tATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcgt < 1:360453/69‑1 (MQ=255) tATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcgt < 1:1488525/69‑1 (MQ=255) tATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcgt < 1:1610238/69‑1 (MQ=255) tATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcgt < 1:652391/69‑1 (MQ=255) tATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcgt < 1:1718869/69‑1 (MQ=255) tATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcgt < 1:1730500/69‑1 (MQ=255) tATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcgtcgt < 1:1879665/69‑1 (MQ=255) tATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCcggcgt < 1:1315875/69‑1 (MQ=255) tGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAg > 1:728492/1‑44 (MQ=255) tGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:118026/1‑69 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCTTCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:1410680/1‑67 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCATCTTCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:3116243/1‑67 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:1494856/1‑67 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:682053/1‑67 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:729092/1‑67 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:836992/1‑67 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:2933027/1‑67 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:1597128/1‑67 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:3093686/1‑67 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:3371830/1‑67 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:3358056/1‑67 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:3294567/1‑67 (MQ=255) cAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTaa > 1:3241930/1‑67 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:1680129/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:858127/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:237388/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:1221119/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:739413/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:559980/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:1671549/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:2773301/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:588905/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:2861006/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:3160841/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:2956296/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:1344771/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAt < 1:3356250/69‑1 (MQ=255) cAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCCTAACGCAGAt < 1:2308262/69‑1 (MQ=255) | TTCAAAGCCGTGATTAACCACGTTGCCAGCGGTGAACGGCGAGAACTTACCAAAGGTATGCAGTTCAATTTTGTCGCCCAGCGCCATCATCTCGCGTACCAGTTGCTGGTTAGTTTTGCCGTCGTAACGCAGAT > minE/1339795‑1339928 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |