| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 106,166 | C→T | 31.2% | Q42Q (CAG→CAA) | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 106,166 | 0 | C | T | 31.2% | 45.2 / 21.2 | 51 | Q42Q (CAG→CAA) | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base C (18/17); new base T (9/7); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAA > minE/106098‑106233 | tGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGAc < 1:1274692/69‑1 (MQ=255)tGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGAc < 1:147252/69‑1 (MQ=255) gCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTg < 1:3049280/67‑1 (MQ=255) gCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTg < 1:2448169/67‑1 (MQ=255) gCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTg < 1:355016/67‑1 (MQ=255) gCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTg < 1:455473/67‑1 (MQ=255) gCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTg < 1:18215/67‑1 (MQ=255) gctaTAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGATTGTATTACGGAGGCGCTa > 1:2136181/4‑69 (MQ=255) gCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGATTGTATTACGGAGGCGCTa > 1:899767/1‑69 (MQ=255) gCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGATTGTATTACGGAGGCGCTa > 1:3184020/1‑69 (MQ=255) gCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGATTGTATTACGGAGGCGCTa > 1:2265750/1‑69 (MQ=255) gCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGATTGTATTACGGAGGCGCTa > 1:532440/1‑69 (MQ=255) gCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGATTGTATTACGGAGGCGCTa > 1:567347/1‑69 (MQ=255) gCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGATTGTATTACGGAGGCGCTa > 1:1947104/1‑69 (MQ=255) gCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGATTGTATTACGGAGGCGCTa > 1:1687740/1‑69 (MQ=255) gCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGATTGTATTACGGAGGCGCTa > 1:1452527/1‑69 (MQ=255) aGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATaa < 1:1761498/69‑1 (MQ=255) aGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATaa < 1:2122008/69‑1 (MQ=255) aGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATaa < 1:1236755/69‑1 (MQ=255) aaTGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAAccc > 1:2425662/1‑69 (MQ=255) ggCGTAACCCAGGATAATCCCTGGCCCTGCGGTCTg < 1:853366/36‑1 (MQ=255) ggCGTAACCCAGGATAATCCCTGGCCCTGCGGACTg < 1:3104866/36‑1 (MQ=255) ggCGTAACCCAGGATAATCCCTGGCCCTGCGGACTg < 1:1529843/36‑1 (MQ=255) ggCGTAACCCAGGATAATCCCTGGCCCTGCGGACTg < 1:886368/36‑1 (MQ=255) ggCGTAACCCAGGATAATCCCTGGCCCTGCGGACTg < 1:1205991/36‑1 (MQ=255) ggCGTAACCCAGGATAATCCCTGGCCCTCCGGACTg < 1:2420230/36‑1 (MQ=255) gCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGt > 1:2687180/1‑68 (MQ=255) gCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTc > 1:841509/1‑69 (MQ=255) gCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTc > 1:2506651/1‑69 (MQ=255) gCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTc > 1:1767134/1‑69 (MQ=255) gCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCAgg > 1:850214/1‑67 (MQ=255) aCCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGa > 1:944706/1‑39 (MQ=255) aCCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGa > 1:2925383/1‑39 (MQ=255) aCCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGa > 1:2696364/1‑39 (MQ=255) aCCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGa > 1:122908/1‑39 (MQ=255) aCCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGa > 1:2025663/1‑39 (MQ=255) cccTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCa > 1:1093255/1‑68 (MQ=255) ccTGCGGATTGTATTACGGAGGCGCTACCCAGGAATAGCCCTGTCCCTATCGCGCCACCCAGCGCGATa < 1:2289666/69‑1 (MQ=255) ccTGCGGATTGTATTACGGAGGCGCTACCCAGGAATAGCCCTGTCCCTATCGCGCCACCCAGCGCGATa < 1:1002337/69‑1 (MQ=255) ccTGCGGATTGTATTACGGAGGCGCTACCCAGGAATAGCCCTGTCCCTATCGCGCCACCCAGCGCGATa < 1:1430094/69‑1 (MQ=255) ccTGCGGATTGTATTACGGAGGCGCTACCCAGGAATAGCCCTGTCCCTATCGCGCCACCCAGCGCGATa < 1:1867741/69‑1 (MQ=255) ccTGCGGATTGTATTACGGAGGCGCTACCCAGGAATAGCCCTGTCCCTATCGCGCCACCCAGCGCGATa < 1:3220486/69‑1 (MQ=255) ccTGCGGATTGTATTACGGAGGCGCTACCCAGGAATAGCCCTGTCCCTATCGCGCCACCCAGCGCGATa < 1:2649859/69‑1 (MQ=255) ccTGCGGATTGTATTACGGAGGCGCTACCCAGGAATAGCCCTGTCCCTATCGCGCCACCCAGAGCGATa < 1:2657/69‑1 (MQ=255) cGGACTGTATTACGGAGGCGCTACCCAGGAATAAccc > 1:3033097/1‑37 (MQ=255) cGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGAt > 1:2198099/1‑64 (MQ=255) cGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGc > 1:3358502/1‑68 (MQ=255) cGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGc > 1:2225960/1‑68 (MQ=255) cGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGc > 1:1743358/1‑68 (MQ=255) cGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGc > 1:2784717/1‑68 (MQ=255) aCTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGaa < 1:329739/69‑1 (MQ=255) aCTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGaa < 1:279906/69‑1 (MQ=255) aCTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGaa < 1:3215153/69‑1 (MQ=255) | TGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATCCCTGGCCCTGCGGACTGTATTACGGAGGCGCTACCCAGGAATAACCCGGTCCCTATCGCGCCACCCAGCGCGATAAGCTGAA > minE/106098‑106233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |