| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,341,374 | A→G | 22.0% | G210G (GGT→GGC) | wcaA ← | predicted glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,341,374 | 0 | A | G | 22.0% | 138.3 / 31.2 | 104 | G210G (GGT→GGC) | wcaA | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (74/7); new base G (17/6); total (91/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.63e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
TGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACC > minE/1341312‑1341440 | tgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGtg < 1:1298368/68‑1 (MQ=255) tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGtgattga < 1:3147311/69‑1 (MQ=255) tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGtgattga < 1:1539297/69‑1 (MQ=255) tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGtgattga < 1:2066665/69‑1 (MQ=255) tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGtgattga < 1:1336075/69‑1 (MQ=255) tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGtgattga < 1:654314/69‑1 (MQ=255) ggAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCt > 1:1020898/1‑69 (MQ=255) ggAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCt > 1:1049099/1‑69 (MQ=255) tACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGtgattga > 1:1070945/1‑52 (MQ=255) tccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGtgattga < 1:553651/49‑1 (MQ=255) tccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGtgattga < 1:3259132/49‑1 (MQ=255) tccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGtgattga < 1:3124739/49‑1 (MQ=255) tccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGtgattga < 1:1255362/49‑1 (MQ=255) tccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGtgattga < 1:2985170/49‑1 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACtt > 1:43653/1‑69 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACtt > 1:976434/1‑69 (MQ=255) ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACtt > 1:2264907/1‑69 (MQ=255) aGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGAGTCGCCTCTTCTACTTTCCACGGTTCGc < 1:3201888/69‑1 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCg > 1:2948342/1‑43 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGc > 1:366444/1‑44 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc > 1:339740/1‑68 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:443295/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:1406661/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:2491763/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:5475/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:2548594/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:2976091/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:177184/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:1641636/1‑69 (MQ=255) gTGATCTGCAATTCACCGTGATTGATATGCAGGATCTGCGTCGc > 1:1689686/1‑44 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTGCACGGTTCGCCGTa > 1:3423517/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGt > 1:581454/1‑68 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGt > 1:786539/1‑68 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:976208/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:1124606/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:250933/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:603062/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:635984/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:711856/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:691225/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTAGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:1975037/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTc > 1:3261401/1‑54 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGc > 1:1103799/1‑64 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:2061288/1‑68 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:2002160/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:2610203/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:1573874/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:494606/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:2705143/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:2777104/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:1207761/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCtcttct > 1:1317789/1‑46 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGtt > 1:751712/1‑59 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:635823/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:3151705/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:1826691/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:249609/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:2955262/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:431753/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:2066583/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACt > 1:266610/1‑68 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:640588/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:371430/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:518112/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:1561117/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:3389231/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:2194388/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:2980338/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:293719/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:2672746/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:2513498/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:1845306/1‑69 (MQ=255) gCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTc > 1:760392/1‑50 (MQ=255) gCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc > 1:2602484/1‑68 (MQ=255) gCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc > 1:2538770/1‑68 (MQ=255) gCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc > 1:25371/1‑68 (MQ=255) gCATTACACCGTGATAGATATGCAGGAACTGCGTCGCctcta > 1:604647/1‑41 (MQ=25) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCGACTTTCCACGGTTCGCCg > 1:2826913/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCAc > 1:1492146/1‑45 (MQ=38) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:1890135/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:2230988/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:692060/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:772460/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:1624842/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:973828/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:1086678/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:1429117/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:1236393/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:2338075/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:2811214/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:1252019/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:3243428/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCAAGGTTCGCCg > 1:1639953/1‑61 (MQ=38) tttCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc > 1:1247072/1‑68 (MQ=255) tCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACgg > 1:767352/1‑50 (MQ=255) tCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc > 1:1928415/1‑69 (MQ=255) tCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc > 1:694611/1‑69 (MQ=255) tCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc > 1:734359/1‑69 (MQ=255) tCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc > 1:586724/1‑69 (MQ=255) tCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc > 1:2745658/1‑69 (MQ=255) | TGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACC > minE/1341312‑1341440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |