| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,341,410 | T→C | 22.6% | V198V (GTA→GTG) | wcaA ← | predicted glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,341,410 | 0 | T | C | 22.6% | 158.1 / 38.2 | 120 | V198V (GTA→GTG) | wcaA | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base T (76/16); new base C (16/11); total (93/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
TTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCC > minE/1341346‑1341464 | ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACtt > 1:2264907/1‑69 (MQ=255)ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACtt > 1:43653/1‑69 (MQ=255) aGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGAGTCGCCTCTTCTACTTTCCACGGTTCGc < 1:3201888/69‑1 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:2491763/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:177184/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:1641636/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:2976091/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:1406661/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:443295/1‑69 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:5475/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTGCACGGTTCGCCGTa > 1:3423517/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGt > 1:581454/1‑68 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGt > 1:786539/1‑68 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:976208/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:250933/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:1124606/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:711856/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:635984/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:603062/1‑69 (MQ=255) gATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTAGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:1975037/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTc > 1:3261401/1‑54 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGc > 1:1103799/1‑64 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa > 1:2061288/1‑68 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:1207761/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:494606/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:2002160/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:2777104/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:2705143/1‑69 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc > 1:2610203/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCtcttct > 1:1317789/1‑46 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:1826691/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:2955262/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:635823/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:2066583/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:3151705/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg > 1:431753/1‑64 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACt > 1:266610/1‑68 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:2194388/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:293719/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:2980338/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:1845306/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:1561117/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:2672746/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:3389231/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:371430/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:2513498/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:518112/1‑69 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc > 1:640588/1‑69 (MQ=255) gCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTc > 1:760392/1‑50 (MQ=255) gCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc > 1:25371/1‑68 (MQ=255) gCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc > 1:2538770/1‑68 (MQ=255) gCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc > 1:2602484/1‑68 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCAc > 1:1492146/1‑45 (MQ=38) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:1429117/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:692060/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:2230988/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:1624842/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:1252019/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:1236393/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:973828/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:2811214/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:2338075/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:1086678/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCg > 1:772460/1‑61 (MQ=255) cATTTCGCCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCCACTTTCCAAGGTTCGCCg > 1:1639953/1‑61 (MQ=38) tttCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc > 1:1247072/1‑68 (MQ=255) tCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACgg > 1:767352/1‑50 (MQ=255) tCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc > 1:2745658/1‑69 (MQ=255) tCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc > 1:734359/1‑69 (MQ=255) tCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc > 1:694611/1‑69 (MQ=255) tCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc > 1:1928415/1‑69 (MQ=255) tgatATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa < 1:1847997/69‑1 (MQ=255) tgatATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa < 1:1171814/69‑1 (MQ=255) tgatATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa < 1:3188167/69‑1 (MQ=255) tgatATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa < 1:2074222/69‑1 (MQ=255) tgatATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa < 1:1695686/69‑1 (MQ=255) tgatATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa < 1:911972/69‑1 (MQ=255) tgatATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa < 1:2747667/69‑1 (MQ=255) tgatATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa < 1:651213/69‑1 (MQ=255) gatATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa > 1:885175/1‑65 (MQ=255) gatATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:2378183/69‑1 (MQ=255) gatATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:692339/69‑1 (MQ=255) gatATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:1594794/69‑1 (MQ=255) gatATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa < 1:3302098/68‑1 (MQ=255) gatATGCAGGATCTGCGTCGCCTCTTCCACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa < 1:1517668/68‑1 (MQ=255) atatGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:110039/68‑1 (MQ=255) tCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCAt > 1:2724643/1‑49 (MQ=255) tCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa > 1:780945/1‑68 (MQ=255) tCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt > 1:1282013/1‑69 (MQ=255) tCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt > 1:3353692/1‑69 (MQ=255) tCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt > 1:3188524/1‑69 (MQ=255) tCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt > 1:2517670/1‑69 (MQ=255) tCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt > 1:2287828/1‑69 (MQ=255) tCTGCCTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt > 1:3234961/1‑69 (MQ=255) tCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa < 1:1339541/52‑1 (MQ=255) tCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATcc < 1:1348034/65‑1 (MQ=255) tCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATcc < 1:1372739/65‑1 (MQ=255) tCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATcc < 1:336750/65‑1 (MQ=255) tCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATcc < 1:1122629/65‑1 (MQ=255) tCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATcc < 1:2097443/65‑1 (MQ=255) | TTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCC > minE/1341346‑1341464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |