| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,341,659 | T→A | 100% | T115T (ACA→ACT) | wcaA ← | predicted glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,341,659 | 0 | T | A | 77.2% | 54.7 / 12.8 | 35 | T115T (ACA→ACT) | wcaA | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base T (7/1); new base A (19/8); total (26/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTC > minE/1341616‑1341718 | cTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCg > 1:111669/1‑68 (MQ=255)cTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCg > 1:2488445/1‑68 (MQ=255)cTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCg > 1:295009/1‑68 (MQ=255) gCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACg > 1:1522388/1‑67 (MQ=255) gCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACg > 1:2262721/1‑67 (MQ=255) gCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACg > 1:2364468/1‑67 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGtt > 1:1371967/1‑67 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:935436/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:347257/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:3411165/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:3242030/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:314112/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2988958/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:960683/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2897721/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2895094/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2679188/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:989384/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:1447229/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:1699186/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2035548/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2034128/1‑69 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTg > 1:347211/1‑68 (MQ=255) cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTAgg > 1:2452965/1‑69 (MQ=255) cGTTAGCGTACAAAAAGCCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2587561/1‑69 (MQ=255) gTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGGGTCCa < 1:2523805/69‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:3197606/68‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:12258/68‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:145253/68‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:2636382/68‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:2372719/68‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:2646389/68‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTACGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:36516/68‑1 (MQ=255) cAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtc > 1:2969983/1‑69 (MQ=255) ggCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:2886680/59‑1 (MQ=255) tgtgtgACCAGTTGCTGTTTATTGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgt > 1:630451/1‑61 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTTTCCATtcgtc > 1:452599/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:1011528/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:972908/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:2712766/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:3386243/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:3357022/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:2300057/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:1815452/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:3087243/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGATCAGACGGTTGGGAGTCCATtcgtc > 1:165489/1‑62 (MQ=255) | CTTCGCCCTGGCAAACGTAGTCGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTC > minE/1341616‑1341718 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |