| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,341,698 | A→G | 36.9% | R102R (CGT→CGC) | wcaA ← | predicted glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,341,698 | 0 | A | G | 36.9% | 39.3 / 44.6 | 73 | R102R (CGT→CGC) | wcaA | predicted glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (26/20); new base G (19/8); total (45/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01 | |||||||||||
CGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGATATATTCCCCTTGC > minE/1341637‑1341752 | cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGtt > 1:1371967/1‑67 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:3242030/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2679188/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2895094/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2897721/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2035548/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2034128/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2988958/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:314112/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:3411165/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:1699186/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:347257/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:935436/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:989384/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:960683/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:1447229/1‑69 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTg > 1:347211/1‑68 (MQ=255)cGTTAGCGTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTAgg > 1:2452965/1‑69 (MQ=255)cGTTAGCGTACAAAAAGCCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTgg > 1:2587561/1‑69 (MQ=255) gTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGGGTCCa < 1:2523805/69‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:12258/68‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:145253/68‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:2646389/68‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:2636382/68‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:2372719/68‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:3197606/68‑1 (MQ=255) gTACAAAAAGGCGTGAGTGACCAGTTGCTGTTTACGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:36516/68‑1 (MQ=255) cAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtc > 1:2969983/1‑69 (MQ=255) ggCGTGAGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGGCGGTTGGGTGTcc < 1:2886680/59‑1 (MQ=255) tgtgtgACCAGTTGCTGTTTATTGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgt > 1:630451/1‑61 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTTTCCATtcgtc > 1:452599/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:972908/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:3087243/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:2712766/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:3357022/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:3386243/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:1011528/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:2300057/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgtc > 1:1815452/1‑62 (MQ=255) tgtgtgACCAGTTGCTGTTTATGGGCGAGGAAGACGATCAGACGGTTGGGAGTCCATtcgtc > 1:165489/1‑62 (MQ=255) aCCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgt > 1:2045563/1‑55 (MQ=255) aCCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgt > 1:2003202/1‑55 (MQ=255) aCCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgt > 1:936255/1‑55 (MQ=255) aCCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgt > 1:646910/1‑55 (MQ=255) aCCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgt > 1:529970/1‑55 (MQ=255) aCCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgt > 1:1607085/1‑55 (MQ=255) aCCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATtcgt > 1:1901758/1‑55 (MQ=255) aCCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCAAtcgt > 1:2650620/1‑55 (MQ=255) tGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTg < 1:1977865/69‑1 (MQ=255) tGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTg < 1:453922/69‑1 (MQ=255) tGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTg < 1:49371/69‑1 (MQ=255) gCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGgtgta < 1:1256483/41‑2 (MQ=255) gCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTc < 1:1062394/41‑1 (MQ=255) gCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTc < 1:907011/41‑1 (MQ=255) gCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTc < 1:3020485/41‑1 (MQ=255) gCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTc < 1:1971455/41‑1 (MQ=255) gCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGa < 1:1848446/69‑1 (MQ=255) gCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGa < 1:1046048/69‑1 (MQ=255) gCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGa < 1:872098/69‑1 (MQ=255) gCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGa < 1:430835/69‑1 (MQ=255) gCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGa < 1:1831644/69‑1 (MQ=255) gCTGTTTATGGGCGAGGAAGACGCTCAGACGGGTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGa < 1:514618/69‑1 (MQ=255) tATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGatatat < 1:1364812/68‑1 (MQ=255) tATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGatatat < 1:658621/68‑1 (MQ=255) tATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGatatat < 1:1719137/68‑1 (MQ=255) tATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGatatat < 1:333489/68‑1 (MQ=255) tATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGATATAtt > 1:1349685/1‑69 (MQ=255) tATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGATATAtt > 1:3204552/1‑69 (MQ=255) tATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGATATAtt > 1:102471/1‑69 (MQ=255) tATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATCCGTCATCGTCATCAATCCCGGTGATATAtt > 1:1895783/1‑69 (MQ=255) ggCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGATATATTccc < 1:1932441/68‑1 (MQ=255) gAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGATATATTCCCCTTgc > 1:661292/1‑67 (MQ=255) gAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGATATATTCCCCTTgc > 1:977093/1‑67 (MQ=255) | CGTTAGCGTACAAAAAGGCGTGTGTGACCAGTTGCTGTTTATGGGCGAGGAAGACGCTCAGACGGTTGGGTGTCCATTCGTCATCGTCATCAATCCCGGTGATATATTCCCCTTGC > minE/1341637‑1341752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |