Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,343,785	G→A	72.0%	D158D (GAC→GAT)	wzc ←	protein‑tyrosine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,343,785	0	G	A	72.0%	28.4 / 10.1	32	D158D (GAC→GAT)	wzc	protein‑tyrosine kinase
Reads supporting (aligned to +/- strand):  ref base G (5/4);  new base A (14/9);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CATCTGGCCCGCTTGCCCCCGGGCGCTAAAGCCGCCGTCGCTGCTCAGGGTGTAGTTTTTGTTGTCCAGCACATTAAGCGTAAACACCTGATCCGCCATCTCTTT  >  minE/1343722‑1343826                                                                |                                          cATCTGGGCCGCTTGCCCCCGGGCGCTAAAGCCGCCGTCGCTGCTCAGGGTGTAGTTTTTGTTGTCCAg                                      >  1:1527271/1‑69 (MQ=255) cATCTGGCCCGCTTGCCCCCGGGCGCTAAAGCCGCCGTCGCTGCTCAGGGTGTAGTTTTTGTTGTCCAg                                      >  1:2947797/1‑69 (MQ=255) cATCTGGCCCGCTTGCCCCCGGGCGCTAAAGCCGCCGTCGCTGCTCAGGGTGTAGTTTTTGTTGTCCAg                                      >  1:3222605/1‑69 (MQ=255) cATCTGGCCCGCTTGCCCCCGGGCGCTAAAGCCGCCGTCGCTGCTCAGGGTGTAGTTTTTGTTGTCCAg                                      >  1:1919497/1‑69 (MQ=255) cATCTGGCCCGCTTGCCCCCGGGCGCTAAAGCCGCCGTCGCTGCTCAGGGTGTAGTTTTTGTTGTCCAg                                      >  1:2117018/1‑69 (MQ=255)              tGCCCCCGGGCGCTAAAGCCGCCGTCGCTGCTCAGGGTGTAGTTTTTGTTGTCCAGCACATTAAGCGTa                         <  1:1379035/69‑1 (MQ=255)              tGCCCCCGGGCGCTAAAGCCGCCGTCGCTGCTCAGGGTGTAGTTTTTGTTGTCCAGCACATTAAGCGTa                         <  1:671071/69‑1 (MQ=255)              tGCCCCCGGGCGCTAAAGCCGCCGTCGCTGCTCAGGGTGTAGTTTTTGTTGTCCAGCACATTAAGCGTa                         <  1:2273741/69‑1 (MQ=255)                   acGGGCGCTAAAGCCGCCATCACTGCTCAGGGTGTAGTTTTTGTCATCAAGCACATTAAGCGTAAaca                     <  1:503581/67‑1 (MQ=255)                   acGGGCGCTAAAGCCGCCATCACTGCTCAGGGTGTAGTTTTTGTCATCAAGCACATTAAGCGTAAaca                     <  1:2891333/67‑1 (MQ=255)                   acGGGCGCTAAAGCCGCCATCACTGCTCAGGGTGTAGTTTTTGTCATCAAGCACATTAAGCGTAAaca                     <  1:588650/67‑1 (MQ=255)                   acGGGCGCTAAAGCCGCCATCACTGCTCAGGGTGTAGTTTTTGTCATCAAGCACATTAAGCGTAAaca                     <  1:1254436/67‑1 (MQ=255)                   acGGGCGCTAAAGCCGCCATCACTGCTCAGGGTGTAGTTTTTGTCATCAAGCACATTAAGCGTAAaca                     <  1:2645669/67‑1 (MQ=255)                   acGGGCGCTAAAGCCGCCATCACTGCTCAGGGTGTAGTTTTTGTCATCAAGCACATTAAGCGTAAaca                     <  1:2621348/67‑1 (MQ=255)                   acGGGCGCTAAAGCCGCCATCACTGCTCAGGGTGTAGTTTTTGTCATCAAGCACATTAAGCGTAAaca                     <  1:1993235/67‑1 (MQ=255)                   acGGGCGCTAAAGCCGCCATCACTGCTCAGGGTGTAGTTTTTGTCATCAAGCACATTAAGCGTAAaca                     <  1:1703997/67‑1 (MQ=255)                    cGGGCGCTAAAGCCGCCATCACTGCTCAGGGTGTAGTTTTTGTCATCAAGCACATTAAGCGTAAaca                     <  1:3060675/67‑1 (MQ=255)                      ggCGCTAAAGCCGCCGTCGCTGCTCAGGGTGTAGTTTTTGTTGTCCAGCACATTAAGCGTAAACACCTg                 <  1:1366029/69‑1 (MQ=255)                                                          tttGTCATCGAGCACATTAAGCGTAAACACCCGATCCGCCATCTCttt  >  1:2781401/1‑48 (MQ=37)                                                          tttGTCATCAAGCACATTAAGCGTAAACACCCGATCCGCCATCTCttt  >  1:417860/1‑48 (MQ=37)                                                          tttGTCATCAAGCACATTAAGCGTAAACACCCGATCCGCCATCTCttt  >  1:625517/1‑48 (MQ=37)                                                          tttGTCATCAAGCACATTAAGCGTAAACACCCGATCCGCCATCTCttt  >  1:2826003/1‑48 (MQ=37)                                                          tttGTCATCAAGCACATTAAGCGTAAACACCCGATCCGCCATCTCttt  >  1:720281/1‑48 (MQ=37)                                                          tttGTCATCAAGCACATTAAGCGTAAACACCCGATCCGCCATCTCttt  >  1:853947/1‑48 (MQ=37)                                                          tttGTCATCAAGCACATTAAGCGTAAACACCCGATCCGCCATCTCttt  >  1:3386930/1‑48 (MQ=37)                                                          tttGTCATCAAGCACATTAAGCGTAAACACCCGATCCGCCATCTCttt  >  1:3039448/1‑48 (MQ=37)                                                          tttGTCATCAAGCACATTAAGCGTAAACACCCGATCCGCCATCTCttt  >  1:2612938/1‑48 (MQ=37)                                                          tttGTCATCAAGCACATTAAGCGTAAACACCCGATCCGCCATCTCttt  >  1:1638672/1‑48 (MQ=37)                                                          tttGTCATCAAGCACATTAAGCGTAAACACCCGATCCGCCATCTCttt  >  1:1347206/1‑48 (MQ=37)                                                          tttGTCATCAAGCACATTAAGCGTAAACACCCGATCAGCCATCTCttt  >  1:3240336/1‑48 (MQ=255)                                                          tttGTCATCAAGCACATTAAGCGTAAACACCCGATCAGCCATCTCttt  >  1:1529437/1‑48 (MQ=255)                                                          tttGTCATCAAGCACATTAAGAGTAAACACCCGATCCGCCATCTCttt  >  1:2867285/1‑48 (MQ=25)                                                                |                                          CATCTGGCCCGCTTGCCCCCGGGCGCTAAAGCCGCCGTCGCTGCTCAGGGTGTAGTTTTTGTTGTCCAGCACATTAAGCGTAAACACCTGATCCGCCATCTCTTT  >  minE/1343722‑1343826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑